ES2049618B1 - Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi. - Google Patents
Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi.Info
- Publication number
- ES2049618B1 ES2049618B1 ES09102521A ES9102521A ES2049618B1 ES 2049618 B1 ES2049618 B1 ES 2049618B1 ES 09102521 A ES09102521 A ES 09102521A ES 9102521 A ES9102521 A ES 9102521A ES 2049618 B1 ES2049618 B1 ES 2049618B1
- Authority
- ES
- Spain
- Prior art keywords
- trypanosoma cruzi
- classification
- diagnostic method
- species
- parasite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000223109 Trypanosoma cruzi Species 0.000 title abstract 4
- 238000002405 diagnostic procedure Methods 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 244000045947 parasite Species 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO DE DIAGNOSTICO Y CLASIFICACION DE ESPECIES DE TRYPANOSOMA CRUZI. A TRAVES DE UNA SECUENCIA DE NUCLEOTIDOS ES POSIBLE PRODUCIR POR SINTESIS QUIMICA FRAGMENTOS DE OLIGONUCLEOTIDOS DE ESTRUCTURA PARCIAL IDENTICA. ESTOS FRAGMENTOS CONSTITUYEN LAS SONDAS QUE SE UTILIZAN COMO CEBADORES PARA LA AMPLIFICACION DE UN FRAGMENTO MAYOR DEL DNA DEL PARASITO COMPRENDIDO ENTRE LOS OLIGONUCLEOTIDOS SINTETIZADOS. LA DETECCION DE DNA AMPLIFICADO ES UNA DEMOSTRACION DIRECTA DE LA PRESENCIA DEL PARASITO EN LA MUESTRA QUE SE ANALIZA. LA SECUENCIA SE UTILIZA PARA LA DETECCION DE TRYPANOSOMA CRUZI POR PCR (REACCION DE LA POLIMERACION EN CADENA) Y PARA LA CLASIFICACION DE ESPECIES Y VARIEDADES DE TRYPANOSOMA CRUZI.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09102521A ES2049618B1 (es) | 1991-11-13 | 1991-11-13 | Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09102521A ES2049618B1 (es) | 1991-11-13 | 1991-11-13 | Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2049618A1 ES2049618A1 (es) | 1994-04-16 |
| ES2049618B1 true ES2049618B1 (es) | 1994-11-01 |
Family
ID=8274151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09102521A Expired - Lifetime ES2049618B1 (es) | 1991-11-13 | 1991-11-13 | Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2049618B1 (es) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
| US5237016A (en) * | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
| WO1990013668A1 (en) * | 1989-05-05 | 1990-11-15 | Lifecodes Corporation | Method for genetic analysis of a nucleic acid sample |
| AU640745B2 (en) * | 1990-03-30 | 1993-09-02 | Astra Aktiebolag | A novel procedure for the detection of pathogens using dna probes |
-
1991
- 1991-11-13 ES ES09102521A patent/ES2049618B1/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2049618A1 (es) | 1994-04-16 |
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