ES2144466T3 - Metodo de tratamiento de muestras de esputo que contienen mycobacterias. - Google Patents

Metodo de tratamiento de muestras de esputo que contienen mycobacterias.

Info

Publication number
ES2144466T3
ES2144466T3 ES94107052T ES94107052T ES2144466T3 ES 2144466 T3 ES2144466 T3 ES 2144466T3 ES 94107052 T ES94107052 T ES 94107052T ES 94107052 T ES94107052 T ES 94107052T ES 2144466 T3 ES2144466 T3 ES 2144466T3
Authority
ES
Spain
Prior art keywords
compatible
lysis
microbacteria
nucleic acid
buffers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94107052T
Other languages
English (en)
Inventor
James Arthur Down
Adriann Howard Walters
Margaret Sigler Dey
Deborah Renee Howard
Michael Christopher Little
William Edward Keating
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2144466T3 publication Critical patent/ES2144466T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODOS PARA PROCESAR MUESTRAS QUE PUEDEN CONTENER MICROBACTERIAS QUE SON COMPATIBLE CON TANTO LAS TECNICAS DE CULTIVO CONVENCIONALES COMO LOS ANALISIS DE ACIDO NUCLEICO. SE HA DESCUBIERTO QUE EL TRATAMIENTO QUIMICO DURO PREVIAMENTE AUNQUE NECESARIO PARA OBTENER UNA LISIS EFICIENTE SE PUEDE ELIMINAR SIN PERDIDA DE EFICIENCIA DE LISIS, CON LO QUE SE ELIMINAN REACTIVOS QUE PUEDEN INHIBIR REACCIONES DE ACIDO NUCLEICO SUBSIGUIENTES. EL CALOR SOLO ES SUFICIENTE PARA LA LISIS DE LAS MICROBACTERIAS, INCLUSO EN TAMPONES QUE NO CONTIENEN DETERGENTES, QUELANTES, ENZIMAS, ETC. LOS REACTIVOS RESIDUALES INTRODUCIDOS POR METODOS DE PROCESAMIENTO DE MUESTRAS CONVENCIONALES PARA EL CULTIVO PUEDEN ELIMINARSE SUCESIVAMENTE DEL PELLET SIN PERDIDA SIGNIFICANTE DE MICROORGANISMOS POR AL MENOS DOS LAVADOS CON AGUA SALINA, O TAMPONES COMPATIBLES CON ANALISIS DE ACIDO NUCLEICO Y CENTRIFUGACION ADECUADA.
ES94107052T 1993-05-11 1994-05-05 Metodo de tratamiento de muestras de esputo que contienen mycobacterias. Expired - Lifetime ES2144466T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6084493A 1993-05-11 1993-05-11

Publications (1)

Publication Number Publication Date
ES2144466T3 true ES2144466T3 (es) 2000-06-16

Family

ID=22032098

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94107052T Expired - Lifetime ES2144466T3 (es) 1993-05-11 1994-05-05 Metodo de tratamiento de muestras de esputo que contienen mycobacterias.

Country Status (7)

Country Link
US (1) US5554503A (es)
EP (1) EP0626456B1 (es)
JP (1) JP2575290B2 (es)
CA (1) CA2121659C (es)
DE (1) DE69423131T2 (es)
ES (1) ES2144466T3 (es)
SG (1) SG44811A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512440A (en) * 1991-12-18 1996-04-30 Becton Dickinson And Company Process for lysing Mycobacteria
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
AU4586096A (en) * 1995-03-10 1996-09-19 Becton Dickinson & Company Sample processing method for whole blood
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US5811269A (en) * 1996-04-30 1998-09-22 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
JPH1080280A (ja) * 1996-09-09 1998-03-31 Shimadzu Corp 核酸合成法
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6126839A (en) * 1997-02-28 2000-10-03 Kreader; Carol Use of an alcohol-salt buffer wash for the efficient recovery of mycobacteria and mycobacterial DNA from respiratory sediment
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
CA2818763A1 (en) * 2003-09-12 2005-03-31 Biocontrol Systems, Inc. Methods, compositions, and kits for the concentration and detection of microorganisms
CA2670930A1 (en) * 2006-11-29 2008-06-05 Microsens Medtech Ltd Capture of mycobacteria like micro-organisms
JP2008167722A (ja) * 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
WO2012037647A1 (en) 2010-09-24 2012-03-29 David Szellos Buckle system and mounting bracket
WO2017094019A1 (en) * 2015-12-02 2017-06-08 Indian Institute Of Science A method for rapid detection of a single bacterium
CN106048030B (zh) * 2016-06-30 2020-02-07 卢春明 一种检测结核杆菌实验室终末消毒效果的方法
US11174520B2 (en) 2018-02-27 2021-11-16 Delta Electronics, Inc. Method for detecting presence or absence of Mycobacterium and kit thereof
CN110499355A (zh) * 2019-08-27 2019-11-26 深圳市南山区慢性病防治院 一种pcr检测前痰液标本的改良处理方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1317860C (en) * 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
CA2016553A1 (en) * 1989-05-16 1990-11-16 Dyann F. Wirth Dna hybridization probes for identification of mycobacteria
CA2065424C (en) * 1989-09-06 2003-05-27 Jean-Luc Guesdon Fragments of nucleic acids derived from an appropriate mycobacteria genome, their applications in the diagnosis of mycobacteria infections and plasmides containing said fragments
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
US5168039A (en) * 1990-09-28 1992-12-01 The Board Of Trustees Of The University Of Arkansas Repetitive DNA sequence specific for mycobacterium tuberculosis to be used for the diagnosis of tuberculosis
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
DK0528306T3 (da) * 1991-08-15 2000-04-25 Hoffmann La Roche Mycobacterium-primerpar
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria

Also Published As

Publication number Publication date
CA2121659A1 (en) 1994-11-12
DE69423131D1 (de) 2000-04-06
JP2575290B2 (ja) 1997-01-22
EP0626456A1 (en) 1994-11-30
SG44811A1 (en) 1997-12-19
EP0626456B1 (en) 2000-03-01
DE69423131T2 (de) 2000-11-09
JPH06319598A (ja) 1994-11-22
US5554503A (en) 1996-09-10
CA2121659C (en) 2000-11-28

Similar Documents

Publication Publication Date Title
Claus et al. A microbiological examination of some carbonaceous chondrites
BRYAN et al. Two proteins are bound to most species of polysomal mRNA
ES2040199T3 (es) Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.
US5625053A (en) Method of isolating purified plasmid DNA using a nonionic detergent, solution
ES2095565T3 (es) Producto estable en almacen y proceso para aislar rna, dna y proteinas.
ATE117377T1 (de) Methode zur extraktion, amplifikation und zum nachweis einer nukleinsäure aus einer fraktion mononuklearer zellen von peripherem blut.
CA2121659A1 (en) Sample Processing Method for Mycobacteria
AU9058091A (en) Preparation of nucleic acid samples
BR9206985A (pt) Processos para efetuar a manipulação de uma sequência de ácido nucleico para testar uma amostra quanto á presença de um ácido nucleico marcador na mesma vaso de passagem de fluxo aparelho suporte sólido e processo para produzir o mesmo
ES2091005T3 (es) Modificacion de macromoleculas biologicas por microondas.
CA2176496A1 (en) Method for Extracting Nucleic Acids from a Wide Range of Organisms
EP0542790A4 (es)
ES2162875T3 (es) Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.
AR001619A1 (es) Método para amplificacion de secuencias especificas de acido nucleico
ATE171221T1 (de) Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen
ES2080807T3 (es) Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.
NO955119L (no) Salmonella identifisering ved polymerasekjedereaksjon
Rogers et al. Bacterial typing: storing and processing of stabilized reference bacteria for polymerase chain reaction without preparing DNA—an example of an automatable procedure
Christophe et al. Mitochondrial DNA polymerase from wheat embryos
ES2084637T3 (es) Procedimiento para la inactivacion biologica de acidos nucleicos.
Jacobs et al. Detection of a reactive intermediate in the reaction between DNA and hydroxyurea
DE60034673D1 (de) Screeningverfahren auf Anti-Protease Resistenz HIV-2-haltiger Zellen, ausgehend von biologischen Patientenproben
PT1224455E (pt) Processo de separacao de um composto quimico ou biologico numa mistura de compostos similares por difusao num meio tal como um gel
Gordon et al. Heterogeneous binding of high mobility group chromosomal proteins to nuclei
Li et al. Isolation of actinomycetes from soil using extremely high frequency radiation

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 626456

Country of ref document: ES