ES2050277T3 - Metodo de ensayo para un ligando en una muestra. - Google Patents
Metodo de ensayo para un ligando en una muestra.Info
- Publication number
- ES2050277T3 ES2050277T3 ES89909006T ES89909006T ES2050277T3 ES 2050277 T3 ES2050277 T3 ES 2050277T3 ES 89909006 T ES89909006 T ES 89909006T ES 89909006 T ES89909006 T ES 89909006T ES 2050277 T3 ES2050277 T3 ES 2050277T3
- Authority
- ES
- Spain
- Prior art keywords
- binding partner
- specific binding
- ligand
- sample
- optical structure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000010998 test method Methods 0.000 title 1
- 230000009870 specific binding Effects 0.000 abstract 5
- 239000003446 ligand Substances 0.000 abstract 4
- 230000003287 optical effect Effects 0.000 abstract 3
- 239000002184 metal Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000009918 complex formation Effects 0.000 abstract 1
- 239000003989 dielectric material Substances 0.000 abstract 1
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Examining Or Testing Airtightness (AREA)
Abstract
Un método de ensayo de un ligando en una muestra, el cual método comprende las etapas de (a) incubar la muestra en contacto con un compañero de unión específica para el ligando que se desea detectar, estando dicho compañero de unión específica soportado sobre una superficie de una estructura óptica y en presencia de otro reactivo que es o bienun análogo del ligando marcado fluorescentemente o fosforescentemente, específico para el mismo compañero de unión específica, o bienotro compañero de unión específica marcado uorescentemente o fosforescentemente, para el ligando que se desea detectar, comprendiendo dicha estructura óptica un sustrato, una capa de metal, e, interpuesta entre ellas, una capa de material dieléctrico que tiene un índice de refracción inferior al de dicho sustrato, estando dicho compañero de unión específica directa o indirectamente adsorbido sobre o unido a dicha capa de metal; (b) irradiar otra superficie de dicha estructura óptica a un ángulo o margen de ángulos respecto de la normal adecuado, de forma que tenga lugar resonancia de plasmón de superficie de largo alcance y de forma que se genere fluorescencia o fosforescencia; y (c) analizar la fluorescencia o fosforescencia generada para determinar si está alterada por la formación de complejo, y, si se desea, en qué cuantía y a qué velocidad tiene esto lugar.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888817710A GB8817710D0 (en) | 1988-07-25 | 1988-07-25 | Method of assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2050277T3 true ES2050277T3 (es) | 1994-05-16 |
Family
ID=10641075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES89909006T Expired - Lifetime ES2050277T3 (es) | 1988-07-25 | 1989-07-25 | Metodo de ensayo para un ligando en una muestra. |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP0382832B1 (es) |
| JP (1) | JP2969177B2 (es) |
| AU (1) | AU638938B2 (es) |
| CA (1) | CA1339005C (es) |
| DE (1) | DE68914134T2 (es) |
| ES (1) | ES2050277T3 (es) |
| GB (1) | GB8817710D0 (es) |
| IL (1) | IL91105A0 (es) |
| NO (1) | NO177407C (es) |
| WO (1) | WO1990001166A1 (es) |
| ZA (1) | ZA895648B (es) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL9001052A (nl) * | 1990-05-02 | 1991-12-02 | Tno | Werkwijze voor het bepalen van een specifiek ligand in een vloeibaar monster met behulp van een evanescent veld, alsook een daarvoor geschikt onderdeel van de benodigde meetinrichting. |
| DE4024476C1 (es) * | 1990-08-02 | 1992-02-27 | Boehringer Mannheim Gmbh, 6800 Mannheim, De | |
| GB9102646D0 (en) * | 1991-02-07 | 1991-03-27 | Fisons Plc | Analytical device |
| EP0517930B1 (en) * | 1991-06-08 | 1995-05-24 | Hewlett-Packard GmbH | Method and apparatus for detecting the presence and/or concentration of biomolecules |
| EP0575132A1 (en) * | 1992-06-17 | 1993-12-22 | Hewlett-Packard Company | Optical measuring device |
| SE9301270D0 (sv) | 1993-04-19 | 1993-04-17 | Biosensor | |
| US5677196A (en) * | 1993-05-18 | 1997-10-14 | University Of Utah Research Foundation | Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
| US5919712A (en) | 1993-05-18 | 1999-07-06 | University Of Utah Research Foundation | Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
| US5395587A (en) * | 1993-07-06 | 1995-03-07 | Smithkline Beecham Corporation | Surface plasmon resonance detector having collector for eluted ligate |
| US5814565A (en) | 1995-02-23 | 1998-09-29 | University Of Utah Research Foundation | Integrated optic waveguide immunosensor |
| US6611634B2 (en) | 1996-03-19 | 2003-08-26 | University Of Utah Research Foundation | Lens and associatable flow cell |
| US5776785A (en) * | 1996-12-30 | 1998-07-07 | Diagnostic Products Corporation | Method and apparatus for immunoassay using fluorescent induced surface plasma emission |
| DE19715483A1 (de) | 1997-04-14 | 1998-10-15 | Boehringer Mannheim Gmbh | Methode zur gleichzeitigen Bestimmung von biomolekularen Wechselwirkungen mittels Plasmonenresonanz undFluoreszenzdetektion |
| US6222619B1 (en) | 1997-09-18 | 2001-04-24 | University Of Utah Research Foundation | Diagnostic device and method |
| DE102004027957A1 (de) * | 2004-06-08 | 2005-12-29 | Carl Zeiss Jena Gmbh | Verfahren und Anordnung zur Untersuchung der Wechselwirkung von Biomolekülen |
| US20070177150A1 (en) * | 2006-01-27 | 2007-08-02 | Gruhlke Russell W | Surface plasmon resonance biosensor using coupled surface plasmons to decrease width of reflectivity dip |
| EP1818668A1 (en) * | 2006-02-09 | 2007-08-15 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Long range surface plasmon fluorescence spectroscopy for bio-affinity studies |
| JP2008102117A (ja) * | 2006-09-21 | 2008-05-01 | Fujifilm Corp | 表面プラズモン増強蛍光センサおよび蛍光検出方法 |
| WO2017048687A1 (en) * | 2015-09-17 | 2017-03-23 | Gerhard Maale | Sensor device for biosensing and other applications |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58501481A (ja) * | 1981-09-18 | 1983-09-01 | プルーテック リミティド | 光学的導波体による溶液中検査物の測定方法および装置 |
| US4978503A (en) * | 1984-06-13 | 1990-12-18 | Ares-Serono Research & Development Limited Partnership | Devices for use in chemical test procedures |
| GB8415018D0 (en) * | 1984-06-13 | 1984-07-18 | Unilever Plc | Chemical test procedures |
| GB8509491D0 (en) * | 1985-04-12 | 1985-05-15 | Plessey Co Plc | Optic waveguide biosensors |
| US4649280A (en) * | 1985-05-10 | 1987-03-10 | The University Of Rochester | Method and system for the enhancement of fluorescence |
| GB2185308B (en) * | 1986-01-10 | 1989-10-25 | Stc Plc | Optical sensor device |
| GB8705650D0 (en) * | 1987-03-10 | 1987-04-15 | Pa Consulting Services | Assay technique |
| NL8700851A (nl) * | 1987-04-10 | 1988-11-01 | Tno | Werkwijze en inrichting voor het detecteren van zeer lage concentraties van een in een meetmedium aanwezige chemische component onder toepassing van oppervlakte-plasmonresonantie en elektrochemisch gestimuleerde adsorptie. |
| CA1321488C (en) * | 1987-08-22 | 1993-08-24 | Martin Francis Finlan | Biological sensors |
| NL8701998A (nl) * | 1987-08-26 | 1989-03-16 | Univ Leiden | Werkwijze voor het door hydrogenolyse ontleden van chemische afvalstoffen, in het bijzonder organische halogeenverbindingen. |
| GB8813307D0 (en) * | 1988-06-06 | 1988-07-13 | Amersham Int Plc | Biological sensors |
-
1988
- 1988-07-25 GB GB888817710A patent/GB8817710D0/en active Pending
-
1989
- 1989-07-25 CA CA000606539A patent/CA1339005C/en not_active Expired - Fee Related
- 1989-07-25 ZA ZA895648A patent/ZA895648B/xx unknown
- 1989-07-25 DE DE68914134T patent/DE68914134T2/de not_active Expired - Fee Related
- 1989-07-25 EP EP89909006A patent/EP0382832B1/en not_active Expired - Lifetime
- 1989-07-25 IL IL91105A patent/IL91105A0/xx unknown
- 1989-07-25 JP JP1508299A patent/JP2969177B2/ja not_active Expired - Fee Related
- 1989-07-25 EP EP89307581A patent/EP0353937A1/en active Pending
- 1989-07-25 WO PCT/GB1989/000844 patent/WO1990001166A1/en not_active Ceased
- 1989-07-25 ES ES89909006T patent/ES2050277T3/es not_active Expired - Lifetime
- 1989-07-25 AU AU40433/89A patent/AU638938B2/en not_active Ceased
-
1990
- 1990-03-23 NO NO901349A patent/NO177407C/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO901349D0 (no) | 1990-03-23 |
| GB8817710D0 (en) | 1988-09-01 |
| DE68914134D1 (de) | 1994-04-28 |
| JPH03502604A (ja) | 1991-06-13 |
| DE68914134T2 (de) | 1994-08-11 |
| EP0382832B1 (en) | 1994-03-23 |
| IL91105A0 (en) | 1990-03-19 |
| AU638938B2 (en) | 1993-07-15 |
| NO901349L (no) | 1990-05-23 |
| JP2969177B2 (ja) | 1999-11-02 |
| ZA895648B (en) | 1990-06-27 |
| CA1339005C (en) | 1997-03-25 |
| WO1990001166A1 (en) | 1990-02-08 |
| EP0353937A1 (en) | 1990-02-07 |
| EP0382832A1 (en) | 1990-08-22 |
| AU4043389A (en) | 1990-02-19 |
| NO177407C (no) | 1995-09-06 |
| NO177407B (no) | 1995-05-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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