ES2052706T3 - Un sistema de tincion de bacilos resistentes a acidos. - Google Patents

Un sistema de tincion de bacilos resistentes a acidos.

Info

Publication number
ES2052706T3
ES2052706T3 ES88302363T ES88302363T ES2052706T3 ES 2052706 T3 ES2052706 T3 ES 2052706T3 ES 88302363 T ES88302363 T ES 88302363T ES 88302363 T ES88302363 T ES 88302363T ES 2052706 T3 ES2052706 T3 ES 2052706T3
Authority
ES
Spain
Prior art keywords
acid
bacles
resistant
staining system
stain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88302363T
Other languages
English (en)
Inventor
Jayakumar Reuben
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2052706T3 publication Critical patent/ES2052706T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C12Q2304/18—Thionin-type dyes, e.g. Azure, Toluidine Blue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/863—Mycobacterium

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Coloring (AREA)

Abstract

UN SISTEMA DE TEÑIDO PARA BACILOS INALTERABLES AL ACIDO CONTIENE UN TINTE PRIMARIO Y UNO SECUNDARIO; EL TINTE PRIMARIO CONTIENE FUCHSINA BASICA, ALCOHOL ETILICO, ALCOHOL ISOPROPILICO, FENOL Y AGUA, Y EL TINTE SECUNDARIO CONTIENE AZUL DE METILENO, ALCOHOL ETILICO, HIDROXIDO POTASICO, GLICERINA, ACIDO ACETICO GLACIAL, POLIVINILPIRROLIDONA Y AGUA.
ES88302363T 1987-04-06 1988-03-17 Un sistema de tincion de bacilos resistentes a acidos. Expired - Lifetime ES2052706T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/034,237 US4857459A (en) 1987-04-06 1987-04-06 Stain for acid-fast bacilli

Publications (1)

Publication Number Publication Date
ES2052706T3 true ES2052706T3 (es) 1994-07-16

Family

ID=21875145

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88302363T Expired - Lifetime ES2052706T3 (es) 1987-04-06 1988-03-17 Un sistema de tincion de bacilos resistentes a acidos.

Country Status (7)

Country Link
US (1) US4857459A (es)
EP (1) EP0286255B1 (es)
JP (1) JPH0642838B2 (es)
AT (1) ATE82636T1 (es)
CA (1) CA1317862C (es)
DE (1) DE3875997T2 (es)
ES (1) ES2052706T3 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5393661A (en) * 1993-12-22 1995-02-28 Difco Laboratories Three reagent gram staining method and kit
US5827680A (en) * 1994-10-11 1998-10-27 Difco Laboratories Three reagent gram staining method and kit
US6348325B1 (en) 1999-10-29 2002-02-19 Cytyc Corporation Cytological stain composition
US6593102B2 (en) 1999-10-29 2003-07-15 Cytyc Corporation Cytological stain composition
AU1242101A (en) * 1999-10-29 2001-05-14 Cytyc Corporation Cytological stain composition
DE10024086C1 (de) * 2000-05-18 2001-09-27 Merck Patent Gmbh Phenolfreie Färbelösung
CN101126689B (zh) * 2007-08-07 2012-05-16 叶鹰 一种革兰染色二步法
CN103674661A (zh) * 2012-09-12 2014-03-26 无锡飞伊生物科技有限公司 不水洗的革兰染色方法
CN104297038B (zh) * 2014-10-10 2017-05-31 丁昊 结核分枝杆菌荧光抗酸染色染液
AU2018391801B2 (en) * 2017-12-24 2022-12-22 Ventana Medical Systems, Inc. Phenol-free acid-fast staining composition and use thereof
CN113405882B (zh) * 2021-06-18 2023-06-02 大庆志飞生物化工有限公司 一种阿维菌素染色剂及其配制及制片方法
CN113933135B (zh) * 2021-10-13 2024-01-26 郑州安图生物工程股份有限公司 一种用于自动化滴染的革兰氏染色试剂盒和染色方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4692412A (en) * 1972-10-06 1987-09-08 Livingston Virginia W Method of preparing an autogenous vaccine
US4489158A (en) * 1980-05-05 1984-12-18 Montefiore Hospital And Medical Center, Inc. Binding assay for the detection of mycobacteria
US4575484A (en) * 1980-05-05 1986-03-11 Montefiore Medical Center, Inc. Binding assay for the detection of mycobacteria

Also Published As

Publication number Publication date
ATE82636T1 (de) 1992-12-15
CA1317862C (en) 1993-05-18
DE3875997D1 (de) 1992-12-24
EP0286255B1 (en) 1992-11-19
US4857459A (en) 1989-08-15
DE3875997T2 (de) 1993-04-15
EP0286255A3 (en) 1989-10-18
JPH0642838B2 (ja) 1994-06-08
EP0286255A2 (en) 1988-10-12
JPS63263096A (ja) 1988-10-31

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