ES2059401T3 - Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante. - Google Patents

Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante.

Info

Publication number
ES2059401T3
ES2059401T3 ES87400813T ES87400813T ES2059401T3 ES 2059401 T3 ES2059401 T3 ES 2059401T3 ES 87400813 T ES87400813 T ES 87400813T ES 87400813 T ES87400813 T ES 87400813T ES 2059401 T3 ES2059401 T3 ES 2059401T3
Authority
ES
Spain
Prior art keywords
protein
bacterial strain
cultivation
obtaining
mutant bacterial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87400813T
Other languages
English (en)
Inventor
Richard Legoux
Pascal Leplatois
Joseph Evelyne Liauzun
Brigitte Niaudet
Willem Roskam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Elf Sanofi SA
Original Assignee
Elf Sanofi SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elf Sanofi SA filed Critical Elf Sanofi SA
Application granted granted Critical
Publication of ES2059401T3 publication Critical patent/ES2059401T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

ESTE PROCEDIMIENTO CONSISTE EN EMPLEAR UNA CEPA BACTERIANA QUE LLEVA UNA MUTACION LIMITANTE Y HASTA SUPRESORA DE LA EXPRESION DE LOS OPERONES, CUYA TRANSCRIPCION DEPENDE DE LA FIJACION DEL COMPLEJO AMPC-CRP, EN LA QUE HA SIDO INTRODUCIDA UNA SECUENCIA DE ADN CODIFICANTE PARA UN PRECURSOR DE UNA PROTEINA. APLICACION: PRODUCCION DE HGH.
ES87400813T 1986-04-10 1987-04-10 Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante. Expired - Lifetime ES2059401T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8605133A FR2597114B1 (fr) 1986-04-10 1986-04-10 Procede microbiologique pour l'obtention d'une proteine par culture d'une souche bacterienne mutante

Publications (1)

Publication Number Publication Date
ES2059401T3 true ES2059401T3 (es) 1994-11-16

Family

ID=9334099

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87400813T Expired - Lifetime ES2059401T3 (es) 1986-04-10 1987-04-10 Procedimiento microbiologico para la obtencion de una proteina mediante cultivo de una cepa bacteriana mutante.

Country Status (7)

Country Link
US (1) US4945047A (es)
EP (1) EP0245138B1 (es)
JP (4) JP2528872B2 (es)
CA (1) CA1304021C (es)
DE (1) DE3786947T2 (es)
ES (1) ES2059401T3 (es)
FR (1) FR2597114B1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2636644B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide
US5284768A (en) * 1988-08-24 1994-02-08 Sanofi Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide
US5279947A (en) * 1988-08-24 1994-01-18 Sanofi DNA sequence participating in the regulation of the expression of a DNA sequence coding for a precursor of a polypeptide, expression vectors and process for the periplasmic production of the polypeptide
WO2013068445A1 (en) 2011-11-09 2013-05-16 Sanofi Diacylglycerol lipase and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59125895A (ja) * 1983-01-07 1984-07-20 Rikagaku Kenkyusho ヒト生長ホルモン遺伝子の合成法
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
JPS60221091A (ja) * 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JPS60234584A (ja) * 1984-05-09 1985-11-21 Morio Ikehara 組換プラスミド

Also Published As

Publication number Publication date
EP0245138B1 (fr) 1993-08-11
CA1304021C (en) 1992-06-23
US4945047A (en) 1990-07-31
JPH09224684A (ja) 1997-09-02
DE3786947D1 (de) 1993-09-16
EP0245138A1 (fr) 1987-11-11
JPH09224685A (ja) 1997-09-02
FR2597114B1 (fr) 1994-02-25
JP2528872B2 (ja) 1996-08-28
JP2661894B2 (ja) 1997-10-08
JP2708403B2 (ja) 1998-02-04
FR2597114A1 (fr) 1987-10-16
JPS637793A (ja) 1988-01-13
JPH08228789A (ja) 1996-09-10
DE3786947T2 (de) 1994-02-24

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