ES2060142T3 - Procedimiento para la deteccion de mercurio con ayuda de microorganismos estimulados mediante mercurio para proporcionar una bioluminiscencia incrementada. - Google Patents

Procedimiento para la deteccion de mercurio con ayuda de microorganismos estimulados mediante mercurio para proporcionar una bioluminiscencia incrementada.

Info

Publication number
ES2060142T3
ES2060142T3 ES90902150T ES90902150T ES2060142T3 ES 2060142 T3 ES2060142 T3 ES 2060142T3 ES 90902150 T ES90902150 T ES 90902150T ES 90902150 T ES90902150 T ES 90902150T ES 2060142 T3 ES2060142 T3 ES 2060142T3
Authority
ES
Spain
Prior art keywords
mercury
pgl4
microorganisms
bioluminiscence
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90902150T
Other languages
English (en)
Inventor
Horst Molders
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GENLUX FORSCHUNGSGESELLSCHAFT
Original Assignee
GENLUX FORSCHUNGSGESELLSCHAFT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GENLUX FORSCHUNGSGESELLSCHAFT filed Critical GENLUX FORSCHUNGSGESELLSCHAFT
Application granted granted Critical
Publication of ES2060142T3 publication Critical patent/ES2060142T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14003—Alkanal monooxygenase FMN (1.14.14.3), i.e. bacterial-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2520/00—Use of whole organisms as detectors of pollution

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

SELECTIVIDAD DE E. COLI C600 (PGL4) PARA MERCURIO. TASA DE LUMINISCENCIA CONTROL NEGATIVO. SE DESCRIBE UN PROCEDIMIENTO, EN EL QUE SE PUEDEN EMPLEAR MICROORGANISMOS, EQUIPADOS CON VECTOR PLASMIDO, PGL4, CONSTRUIDO MEDIANTE TECNICAS BIOLOGICAS MOLECULARES, COMO BIOSENSORES PARA COMPUESTOS DE MERCURIO. LA BASE DE LA INVENCION ES LA COMBINACION DE UN COMPLEJO-GEN (MER-OPERON) INDUCIBLE MEDIANTE IONES DE MERCURIO CON EL SISTEMA DE GEN LUCEFERASA (LUX) DE VIBRIO-HARVEYI MEDIANTE LA TECNICA DE CRONACION MOLECULAR. EN ESTE CASO, LA DISPOSICION DE AMBAS UNIDADES DE GEN ESTA CONCEBIDA DE FORMA QUE EL PROMOTOR DEL MER-OPERON REGULADO POSITIVAMENTE MEDIANTE COMPUESTOS DE MERCURIO CONTROLA TAMBIEN LA TRANSCRIPCION DE LOS GENES-LUX. COMO CONSECUENCIA, LOS MICROORGANISMOS TRANSFORMADOS CON PLASMIDO PGL4 RESPONDEN AL INCREMENTO DE LAS CANTIDADES DE CATIONES DE MERCURIO EN EL MEDIO CIRCUNDANTE CON UN AUMENTO EN SU BIOLUMINISCENCIA EMITIDA. EL PROCESO ES ABSOLUTAMENTE ESPECIFICO DEL MERCURIO, RAPIDO Y ALTAMENTE SENSIBLE: EL LIMITE PROBADO PARA IONES DE MERCURIO ES DE APROXIMADAMENTE 0,2 PPB, ES DECIR, 5 VECES INFERIOR AL UMBRAL DE DETECCION DE LOS METODOS MAS POTENTES ESTABLECIDOS (ESPECTROMETRIA DE ABSORCION ATOMICA), QUE DETECTAN DE 1 A 5 PPB DE MERCURIO Y REQUIEREN UNA CANTIDAD CONSIDERABLE DE APARATOS. OTRA VENTAJA DE LA INVENCION ES LA POSIBILIDAD DE DETERMINAR VALORES DE MEDICION (SEÑALES DE BIOLUMINISCENCIA EMITIDAS POR MICROORGANISMOS TRANSFORMADOS POR PLASMIDO PGLK4) SIN CONTACTO DIRECTO ENTRE EL BIOSENSOR Y LA UNIDAD AMPLIFICADORA DE LAS SEÑALES (APARATO DE BIOLUMINISCENCIA). POR TANTO, EL METODO PERMITE LA DETERMINACION SENCILLA, CONTINUA, EN-LINEA DE CONCENTRACIONES CRITICAS DE MERCURIO.
ES90902150T 1989-02-01 1990-02-01 Procedimiento para la deteccion de mercurio con ayuda de microorganismos estimulados mediante mercurio para proporcionar una bioluminiscencia incrementada. Expired - Lifetime ES2060142T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3902982A DE3902982A1 (de) 1989-02-01 1989-02-01 Verfahren zum nachweis von quecksilber mit hilfe von durch quecksilber zu erhoehter biolumineszenz angeregter mikroorganismen

Publications (1)

Publication Number Publication Date
ES2060142T3 true ES2060142T3 (es) 1994-11-16

Family

ID=6373229

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90902150T Expired - Lifetime ES2060142T3 (es) 1989-02-01 1990-02-01 Procedimiento para la deteccion de mercurio con ayuda de microorganismos estimulados mediante mercurio para proporcionar una bioluminiscencia incrementada.

Country Status (9)

Country Link
EP (1) EP0456667B1 (es)
JP (1) JPH04503003A (es)
AT (1) ATE91723T1 (es)
AU (1) AU644354B2 (es)
DE (1) DE3902982A1 (es)
DK (1) DK0456667T3 (es)
ES (1) ES2060142T3 (es)
FI (1) FI913611A7 (es)
WO (1) WO1990008836A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0573500B2 (en) * 1991-03-01 2002-05-02 Vito Fused genes and their use for determining the presence of metals or of xenobiotic compounds
WO1993003179A1 (en) * 1991-07-30 1993-02-18 Bio-Technical Resources Device for detecting aqueous contaminants
IL107815A (en) * 1992-12-04 2003-12-10 Du Pont Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing
FI95484C (fi) * 1994-01-17 1996-02-12 Marko Virta Menetelmä näytteessä olevan metallin määrittämiseksi
JPH10509049A (ja) * 1994-11-23 1998-09-08 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 毒物検出のための凍結乾燥生物発光細菌試薬
US6025131A (en) 1996-10-23 2000-02-15 E. I. Du Pont De Namours And Company Facile method for identifying regulated promoters
US7132247B1 (en) 1998-09-17 2006-11-07 Regents Of The University Of Minnesota Composite devices incorporating biological material and methods
JP4578597B2 (ja) * 1999-10-01 2010-11-10 株式会社間組 水銀の気化方法、及び汚染土壌又は汚染水の浄化方法、並びに水銀の検出方法
US20030108980A1 (en) * 2000-08-14 2003-06-12 Sayler Gary S. Bioluminescent methods for direct visual detection of environmental compounds
WO2005014805A1 (en) 2003-08-08 2005-02-17 Regents Of The University Of Minnesota A structured material for the production of hydrogen
CN114152601B (zh) * 2021-12-28 2023-09-15 军事科学院军事医学研究院环境医学与作业医学研究所 一种现场快速检测水中汞离子的方法、试剂盒及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4581335A (en) * 1982-12-01 1986-04-08 Texas A&M University System Process for producing a cloned luciferase-synthesizing microorganism
EP0153366A1 (en) * 1983-08-16 1985-09-04 Battelle Development Corporation Bioluminescent chemical system and method for detecting the presence of chemical agents in a medium
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
AU7751687A (en) * 1986-07-22 1988-02-10 Boyce Thompson Institute For Plant Research Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
DE3714612A1 (de) * 1987-04-16 1988-10-27 Bayer Ag Mikroorganismus-stamm und seine verwendung

Also Published As

Publication number Publication date
AU644354B2 (en) 1993-12-09
FI913611A0 (fi) 1991-07-29
EP0456667A1 (de) 1991-11-21
WO1990008836A1 (de) 1990-08-09
EP0456667B1 (de) 1993-07-21
JPH04503003A (ja) 1992-06-04
DK0456667T3 (da) 1993-12-27
FI913611A7 (fi) 1991-07-29
AU4966090A (en) 1990-08-24
ATE91723T1 (de) 1993-08-15
DE3902982A1 (de) 1990-08-02

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