ES2060949T3 - Metodo de diferenciacion de leucocitos utilizando una citometria de flujo. - Google Patents
Metodo de diferenciacion de leucocitos utilizando una citometria de flujo.Info
- Publication number
- ES2060949T3 ES2060949T3 ES90305229T ES90305229T ES2060949T3 ES 2060949 T3 ES2060949 T3 ES 2060949T3 ES 90305229 T ES90305229 T ES 90305229T ES 90305229 T ES90305229 T ES 90305229T ES 2060949 T3 ES2060949 T3 ES 2060949T3
- Authority
- ES
- Spain
- Prior art keywords
- litical
- flow cytometry
- agent
- cytometry
- differentiation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 4
- 210000000265 leukocyte Anatomy 0.000 title abstract 2
- 230000004069 differentiation Effects 0.000 title 1
- 238000000684 flow cytometry Methods 0.000 title 1
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 238000004163 cytometry Methods 0.000 abstract 2
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 abstract 1
- 239000006096 absorbing agent Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 229960005323 phenoxyethanol Drugs 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Ecology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
EL INVENTO SE REFIERE A UN AGENTE LITICO Y UN METODO PARA REALIZAR UN COMPUTO DE SUBPOBLACION DE CELULAS DIFERENCIAL, PARA UTILIZAR POR EJEMPLO, EN CITOMETRIA DE CIRCULACION. EL AGENTE LITICO COMPRENDE UN OXIETANOL AROMATICO, UN AMORTIGUADOR ORGANICO CON UN PK DE CASI 8,5 Y UN DETERGENTE NO IONICO. PREFERENTEMENTE, EL OXIETANOL AROMATICO ES 2-FENOXIETANOL. EL METODO DEL AGENTE LITICO PERMITE LA DETERMINACION DIFERENCIAL DE 5 PARTES DE LAS SUBPOBLACIONES DE CELULAS LEUCOCITOS DE UNA MUESTRA COMPLETA DE SANGRE UTILIZANDO LOS METODOS DE LA CITOMETRIA DE CIRCULACION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35210689A | 1989-05-15 | 1989-05-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2060949T3 true ES2060949T3 (es) | 1994-12-01 |
Family
ID=23383823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES90305229T Expired - Lifetime ES2060949T3 (es) | 1989-05-15 | 1990-05-15 | Metodo de diferenciacion de leucocitos utilizando una citometria de flujo. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5510267A (es) |
| EP (1) | EP0398652B1 (es) |
| JP (1) | JPH0726956B2 (es) |
| AT (1) | ATE109568T1 (es) |
| AU (1) | AU636607B2 (es) |
| CA (1) | CA2016699C (es) |
| DE (1) | DE69011198T2 (es) |
| ES (1) | ES2060949T3 (es) |
Families Citing this family (93)
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| JP2565844B2 (ja) * | 1992-02-07 | 1996-12-18 | アボツト・ラボラトリーズ | 細胞溶解処理条件下で異種細胞集団を正確に計数し,感受性に関する格付けを行う方法 |
| US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
| AU2095992A (en) * | 1992-08-11 | 1994-03-24 | Abbott Laboratories | Flow cytometry sheath reagent for elimination of red cells with preservation of nucleated cells |
| DE69327775T2 (de) * | 1992-11-19 | 2000-06-21 | Sysmex Corp., Kobe | Verfahren zur Vorbehandlung für Blutanalyse |
| US6812032B1 (en) * | 1993-01-21 | 2004-11-02 | Cdc Technologies, Inc. | Apparatus and method for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures |
| JP3355038B2 (ja) * | 1994-08-03 | 2002-12-09 | シスメックス株式会社 | 白血球分類方法 |
| FR2733835B1 (fr) * | 1995-05-03 | 1997-07-18 | Hycel Groupe Lisabio | Procede et dispositif de detection du point de lyse de globules rouges |
| US5639630A (en) * | 1995-05-16 | 1997-06-17 | Bayer Corporation | Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes |
| AU1288497A (en) * | 1995-12-18 | 1997-07-14 | Center For Laboratory Technology, Inc. | Multi-parameter hematology apparatus and method |
| US5817518A (en) * | 1995-12-18 | 1998-10-06 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
| US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| US6025201A (en) * | 1995-12-28 | 2000-02-15 | Bayer Corporation | Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| US5917584A (en) * | 1997-11-21 | 1999-06-29 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| US6304328B1 (en) * | 1998-08-14 | 2001-10-16 | Research Foundation Of State University Of New York, The Office Of Technology Licensing, Suny | Non-contact temperature and concentration measurement on liquid surfaces |
| US6067157A (en) * | 1998-10-09 | 2000-05-23 | University Of Washington | Dual large angle light scattering detection |
| US6200766B1 (en) | 1998-12-03 | 2001-03-13 | Becton Dickinson And Company | Methods and reagents for quantitation of HLA-DR expression on peripheral blood cells |
| US6423505B1 (en) * | 1998-12-03 | 2002-07-23 | Becton Dickinson And Company | Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells |
| US6210969B1 (en) * | 1999-04-28 | 2001-04-03 | Coulter International Corp. | Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood |
| US6214625B1 (en) * | 1999-04-28 | 2001-04-10 | Coulter International Corp. | Composition and method for differentiation of basophils and eosinophils in blood |
| US6232125B1 (en) | 1999-08-09 | 2001-05-15 | Coulter International Corp. | Method and apparatus for differentiating and enumerating leukocytes |
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| US7641856B2 (en) * | 2004-05-14 | 2010-01-05 | Honeywell International Inc. | Portable sample analyzer with removable cartridge |
| US7420659B1 (en) * | 2000-06-02 | 2008-09-02 | Honeywell Interantional Inc. | Flow control system of a cartridge |
| US20060263888A1 (en) * | 2000-06-02 | 2006-11-23 | Honeywell International Inc. | Differential white blood count on a disposable card |
| US7277166B2 (en) * | 2000-08-02 | 2007-10-02 | Honeywell International Inc. | Cytometer analysis cartridge optical configuration |
| US7061595B2 (en) * | 2000-08-02 | 2006-06-13 | Honeywell International Inc. | Miniaturized flow controller with closed loop regulation |
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| US20060137259A1 (en) * | 2004-11-02 | 2006-06-29 | Purvis Harrison G | Temporary railing for a building including dual-use corner pulley and associated methods |
| FR2883972B1 (fr) * | 2005-03-31 | 2007-11-16 | C2 Diagnostics Sa | Procede pour l'analyse d'un echantillon de sang et appareil et reactif pour sa mise en oeuvre |
| EP1875200A1 (en) | 2005-04-29 | 2008-01-09 | Honeywell International Inc. | Cytometer cell counting and size measurement method |
| US8361410B2 (en) * | 2005-07-01 | 2013-01-29 | Honeywell International Inc. | Flow metered analyzer |
| JP4995197B2 (ja) * | 2005-07-01 | 2012-08-08 | ハネウェル・インターナショナル・インコーポレーテッド | 3d流体力学的集束を有する成形カートリッジ |
| CN101253401B (zh) * | 2005-07-01 | 2013-01-02 | 霍尼韦尔国际公司 | 带三维流体动力学集中的模制标本盒 |
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| WO2007075922A2 (en) * | 2005-12-22 | 2007-07-05 | Honeywell International Inc. | Portable sample analyzer cartridge |
| JP5431732B2 (ja) * | 2005-12-29 | 2014-03-05 | ハネウェル・インターナショナル・インコーポレーテッド | マイクロ流体フォーマットにおけるアッセイ実装 |
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| US7816135B2 (en) | 2007-07-05 | 2010-10-19 | Becton, Dickinson And Company | Method of analyzing lymphocytes |
| CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
| US8102161B2 (en) * | 2007-09-25 | 2012-01-24 | Tdk Corporation | Stable output in a switching power supply by smoothing the output of the secondary coil |
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| CN101470108B (zh) | 2007-12-24 | 2013-11-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种对白细胞进行分类的试剂和方法 |
| CN101475754A (zh) * | 2008-01-04 | 2009-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
| EP2247746A4 (en) * | 2008-01-31 | 2012-08-01 | Amyris Inc | METHOD FOR MONITORING METABOLIC WAYS |
| JP2009236798A (ja) * | 2008-03-28 | 2009-10-15 | Sysmex Corp | 好塩基球の分類計数方法 |
| EP2280278A4 (en) * | 2008-05-09 | 2015-04-08 | Sysmex Corp | Blood analyzer, blood analysis method and hemolysis |
| CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
| US20100034704A1 (en) * | 2008-08-06 | 2010-02-11 | Honeywell International Inc. | Microfluidic cartridge channel with reduced bubble formation |
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| CN101750274B (zh) * | 2008-12-17 | 2014-06-25 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
| CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
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| WO2015073952A1 (en) * | 2013-11-15 | 2015-05-21 | University Of Maryland | Integrated and standalone label and reagent-free microfluidic devices and microsystems for differential white blood cell counts |
| KR102535489B1 (ko) | 2014-06-13 | 2023-05-22 | 큐-리네아 에이비 | 미생물 검출 방법 및 특성 규명 방법 |
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| NO340218B1 (en) | 2015-07-10 | 2017-03-20 | Marine Harvest Norway As | Device and method for removing parasites on fish |
| US10215683B2 (en) | 2015-11-02 | 2019-02-26 | Chiranjit Deka | Light scatter based apparatus and methods for hematology analysis using only three detectors |
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| US11511103B2 (en) | 2017-11-13 | 2022-11-29 | Shifamed Holdings, Llc | Intravascular fluid movement devices, systems, and methods of use |
| JP7410034B2 (ja) | 2018-02-01 | 2024-01-09 | シファメド・ホールディングス・エルエルシー | 血管内血液ポンプならびに使用および製造の方法 |
| CN111936905B (zh) | 2018-03-30 | 2023-01-06 | Idexx实验室公司 | 流式细胞仪的激光光学组件 |
| WO2020028537A1 (en) | 2018-07-31 | 2020-02-06 | Shifamed Holdings, Llc | Intravascaular blood pumps and methods of use |
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1990
- 1990-05-14 CA CA002016699A patent/CA2016699C/en not_active Expired - Lifetime
- 1990-05-15 DE DE69011198T patent/DE69011198T2/de not_active Expired - Fee Related
- 1990-05-15 AU AU55037/90A patent/AU636607B2/en not_active Ceased
- 1990-05-15 ES ES90305229T patent/ES2060949T3/es not_active Expired - Lifetime
- 1990-05-15 JP JP2125266A patent/JPH0726956B2/ja not_active Expired - Lifetime
- 1990-05-15 EP EP90305229A patent/EP0398652B1/en not_active Expired - Lifetime
- 1990-05-15 AT AT90305229T patent/ATE109568T1/de not_active IP Right Cessation
-
1992
- 1992-08-24 US US07/934,282 patent/US5510267A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2016699A1 (en) | 1990-11-15 |
| CA2016699C (en) | 2003-11-18 |
| EP0398652B1 (en) | 1994-08-03 |
| DE69011198T2 (de) | 1995-01-19 |
| DE69011198D1 (de) | 1994-09-08 |
| AU636607B2 (en) | 1993-05-06 |
| ATE109568T1 (de) | 1994-08-15 |
| JPH034171A (ja) | 1991-01-10 |
| US5510267A (en) | 1996-04-23 |
| JPH0726956B2 (ja) | 1995-03-29 |
| EP0398652A1 (en) | 1990-11-22 |
| AU5503790A (en) | 1990-11-15 |
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