ES2062029T3 - Procedimiento de purificacion de proteinas. - Google Patents

Procedimiento de purificacion de proteinas.

Info

Publication number
ES2062029T3
ES2062029T3 ES89310648T ES89310648T ES2062029T3 ES 2062029 T3 ES2062029 T3 ES 2062029T3 ES 89310648 T ES89310648 T ES 89310648T ES 89310648 T ES89310648 T ES 89310648T ES 2062029 T3 ES2062029 T3 ES 2062029T3
Authority
ES
Spain
Prior art keywords
mixture
contaminating proteins
streptokinase
group
protein purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89310648T
Other languages
English (en)
Inventor
Christopher Joseph Mannix
Richard Anthony Godwin Smith
Ceris John Lewis
Julian Stanley Harber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Application granted granted Critical
Publication of ES2062029T3 publication Critical patent/ES2062029T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/885—Streptococcus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Iron Core Of Rotating Electric Machines (AREA)
  • Glass Compositions (AREA)
  • Removal Of Specific Substances (AREA)
  • Steroid Compounds (AREA)

Abstract

UN PROCESO PARA LA SEPARACION DE ESTRECTOQUINASA DE PROTEINAS CONTAMINADAS DE UNA MEZCLA CONTENIENDO ESPECTROQUINASA, EL CUAL COMPRENDE TRATAR LA MEZCLA CON UN AGENTE REDUCTOR PARA REDUCIR LOS ENLACES DEL DISULFURO EN LAS PROTEINAS CONTAMINADAS LIBERANDO GRUPOS TIOL, PONER EN CONTACTO LA MEZCLA CON REACTIVO R-X EN DONDE R ES UN GRUPO CAPAZ DE REACCIONAR CON UN GRUPO TIOL LIBRE O ES UNA MATRIZ CONTENIENDO TIOL, Y ADEMAS SEPARAR LAS PROTEINAS CONTAMINADAS RESULTANTES MODIFICADAS QUIMICAMENTE DE LA MEZCLA PARA PRODUCIR ESTREPTOQUINASA EN FORMA SUSTANCIALMENTE LIBRE DE PROTEINAS CONTAMINADAS.
ES89310648T 1988-10-19 1989-10-17 Procedimiento de purificacion de proteinas. Expired - Lifetime ES2062029T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB888824496A GB8824496D0 (en) 1988-10-19 1988-10-19 Process

Publications (1)

Publication Number Publication Date
ES2062029T3 true ES2062029T3 (es) 1994-12-16

Family

ID=10645453

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89310648T Expired - Lifetime ES2062029T3 (es) 1988-10-19 1989-10-17 Procedimiento de purificacion de proteinas.

Country Status (15)

Country Link
US (1) US5334384A (es)
EP (1) EP0365278B1 (es)
JP (1) JP2837467B2 (es)
KR (1) KR0136613B1 (es)
AT (1) ATE103633T1 (es)
AU (1) AU622492B2 (es)
CA (1) CA2000850A1 (es)
DE (1) DE68914242T2 (es)
DK (1) DK515889A (es)
ES (1) ES2062029T3 (es)
GB (1) GB8824496D0 (es)
IE (1) IE63478B1 (es)
NZ (1) NZ231039A (es)
PT (1) PT92024B (es)
ZA (1) ZA897849B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE513042T1 (de) 1998-02-05 2011-07-15 Glaxosmithkline Biolog Sa Tumorassoziierte antigenderivate der mage-familie,nukleinsäuresequenzen, die dafür kodieren, zur herstellung von fusionsproteinen und zusammensetzungen zur impfung
US6388073B1 (en) 2000-07-26 2002-05-14 Shire Us Inc. Method for the manufacture of anagrelide
WO2002030542A2 (en) 2000-10-12 2002-04-18 Robert Corcoran Purification of substance by reaction affinity chromatography
KR20020007231A (ko) * 2001-08-01 2002-01-26 이병철 스트렙토키나제의 정제방법
US20060030574A1 (en) * 2004-08-04 2006-02-09 Shire Holdings Ag Quinazoline derivatives useful for the treatment of peripheral arterial disease and as phosphodiesterase inhibitors
US7700608B2 (en) * 2004-08-04 2010-04-20 Shire Holdings Ag Quinazoline derivatives and their use in the treatment of thrombocythemia
US7910597B2 (en) * 2006-11-28 2011-03-22 Shire Llc Substituted quinazolines
US8304420B2 (en) 2006-11-28 2012-11-06 Shire Llc Substituted quinazolines for reducing platelet count
NZ589557A (en) * 2008-06-04 2012-08-31 Talecris Biotherapeutics Inc Immobilised streptokinase, method and kit for preparing plasmin
HUE025670T2 (en) 2009-03-03 2016-04-28 Grifols Therapeutics Inc A method for producing plasminogen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2784145A (en) * 1955-11-22 1957-03-05 American Cyanamid Co Recovery of streptokinase
US3419472A (en) * 1960-10-24 1968-12-31 American Cyanamid Co Process for preparing streptokinaserich material
US3226304A (en) * 1960-10-24 1965-12-28 American Cyanamid Co High purity streptokinase and process for preparing same
US3255094A (en) * 1963-01-10 1966-06-07 Baxter Laboratories Inc Method for purification of streptokinase
US3444045A (en) * 1966-09-20 1969-05-13 American Cyanamid Co Process for purifying streptokinase
US4381346A (en) * 1979-11-13 1983-04-26 Huasin Syed S Isolation of plasminogen activators useful as therapeutic and diagnostic agents

Also Published As

Publication number Publication date
DK515889A (da) 1990-04-20
DE68914242T2 (de) 1994-07-07
US5334384A (en) 1994-08-02
EP0365278B1 (en) 1994-03-30
DK515889D0 (da) 1989-10-17
JP2837467B2 (ja) 1998-12-16
AU622492B2 (en) 1992-04-09
KR900006512A (ko) 1990-05-08
JPH02128688A (ja) 1990-05-17
PT92024A (pt) 1990-04-30
NZ231039A (en) 1991-07-26
EP0365278A2 (en) 1990-04-25
AU4296489A (en) 1990-04-26
IE63478B1 (en) 1995-04-19
IE893338L (en) 1990-04-19
EP0365278A3 (en) 1991-08-28
GB8824496D0 (en) 1988-11-23
ATE103633T1 (de) 1994-04-15
KR0136613B1 (ko) 1998-04-25
PT92024B (pt) 1995-08-09
CA2000850A1 (en) 1990-04-19
ZA897849B (en) 1990-10-31
DE68914242D1 (de) 1994-05-05

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