ES2066759T3 - Regulador antisentido de la expresion de un gen en celulas de plantas. - Google Patents

Regulador antisentido de la expresion de un gen en celulas de plantas.

Info

Publication number
ES2066759T3
ES2066759T3 ES87302367T ES87302367T ES2066759T3 ES 2066759 T3 ES2066759 T3 ES 2066759T3 ES 87302367 T ES87302367 T ES 87302367T ES 87302367 T ES87302367 T ES 87302367T ES 2066759 T3 ES2066759 T3 ES 2066759T3
Authority
ES
Spain
Prior art keywords
gene
expression
plant cells
sense regulator
regulator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87302367T
Other languages
English (en)
Inventor
Christine K Shewmaker
Jean C Kridl
William R Hiatt
Vic Knauf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27126594&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2066759(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Calgene LLC filed Critical Calgene LLC
Application granted granted Critical
Publication of ES2066759T3 publication Critical patent/ES2066759T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Construcción de ADN recombinante, que incluye una secuencia de ADN de la poligalacturonasa (PG) que se encuentra en el inserto EcoRI del plásmido pCGN1401 (ATCC 67227), o que puede obtenerse por el uso del mencionado inserto como sonda de hibridación.
ES87302367T 1986-03-28 1987-03-19 Regulador antisentido de la expresion de un gen en celulas de plantas. Expired - Lifetime ES2066759T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84567686A 1986-03-28 1986-03-28
US92057486A 1986-10-17 1986-10-17

Publications (1)

Publication Number Publication Date
ES2066759T3 true ES2066759T3 (es) 1995-03-16

Family

ID=27126594

Family Applications (2)

Application Number Title Priority Date Filing Date
ES91109006T Expired - Lifetime ES2165348T3 (es) 1986-03-28 1987-03-19 Regulacion anti-sentido de expresion de genes en celulas vegetales.
ES87302367T Expired - Lifetime ES2066759T3 (es) 1986-03-28 1987-03-19 Regulador antisentido de la expresion de un gen en celulas de plantas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES91109006T Expired - Lifetime ES2165348T3 (es) 1986-03-28 1987-03-19 Regulacion anti-sentido de expresion de genes en celulas vegetales.

Country Status (12)

Country Link
EP (2) EP0240208B1 (es)
JP (3) JP2702921B2 (es)
CN (1) CN87102348A (es)
AR (1) AR241938A1 (es)
AT (2) ATE114168T1 (es)
AU (3) AU618234B2 (es)
CA (2) CA1341601C (es)
DE (2) DE3752338T2 (es)
ES (2) ES2165348T3 (es)
GR (1) GR3015085T3 (es)
IL (1) IL81737A (es)
NZ (1) NZ219472A (es)

Families Citing this family (81)

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GB8626879D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
NZ224787A (en) * 1987-05-26 1990-08-28 Calgene Inc A dna construct for specifically modifying the phenotype of fruit as distinct from other plant tissue
GB8811115D0 (en) * 1988-05-11 1988-06-15 Ici Plc Tomatoes
WO1990008828A2 (en) * 1989-02-02 1990-08-09 Paladin Hybrids Inc. Molecular methods of hybrid seed production
US5728926A (en) * 1988-02-03 1998-03-17 Pioneer Hi-Bred International, Inc. Antisense gene systems of pollination control for hybrid seed production
US5356799A (en) 1988-02-03 1994-10-18 Pioneer Hi-Bred International, Inc. Antisense gene systems of pollination control for hybrid seed production
NZ227835A (en) * 1988-02-03 1992-09-25 Paladin Hybrids Inc Antisense gene systems of pollination control for hybrid seed production
US6198026B1 (en) 1988-02-03 2001-03-06 Pioneer Hi-Bred International, Inc. Molecular methods of hybrid seed production
US6198023B1 (en) 1988-02-03 2001-03-06 Pioneer Hi-Bred International, Inc. Molecular methods of hybrid seed production
DE3810286A1 (de) * 1988-03-25 1989-10-12 Max Planck Gesellschaft Transgene pflanze mit modifizierter physiologie, morphologie und modifiziertem hormonmetabolismus, gewebekulturen dieser pflanze und verfahren zu ihrer herstellung
NL8800756A (nl) * 1988-03-25 1989-10-16 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Genetisch gemanipuleerde plantecellen en planten, alsmede daarvoor bruikbaar recombinant dna.
GB8818706D0 (en) * 1988-08-05 1988-09-07 Ici Plc Dna constructs & plants incorporating them
WO1990002172A1 (en) * 1988-08-18 1990-03-08 Calgene, Inc. Plant elongation factor, promoters, coding sequences and uses
GB8916213D0 (en) * 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom
GB8923716D0 (en) * 1989-10-20 1989-12-06 Ici Plc Dna,constructs,cells and plants derived therefrom
US5744364A (en) * 1989-10-20 1998-04-28 Zeneca Limited PTOM36 constructs and tomato cells transformed therewith
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
FR2658988A1 (fr) * 1990-03-02 1991-09-06 Biosem Betterave resistante a la rhizomanie.
DE69114275T2 (de) * 1990-03-02 1996-06-13 Biocem Regenerierung und genetische transformation der zuckerrübe.
US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
US5585545A (en) * 1990-04-20 1996-12-17 The Regents Of The University Of California Endo-1,4-β-glucanase genes and their use in plants
GB9009307D0 (en) * 1990-04-25 1990-06-20 Ici Plc Dna,constructs,cells and plant derived therefrom
US5260205A (en) * 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
US5668295A (en) * 1990-11-14 1997-09-16 Philip Morris Incorporated Protein involved in nicotine synthesis, DNA encoding, and use of sense and antisense DNAs corresponding thereto to affect nicotine content in transgenic tobacco cells and plants
WO1992009685A1 (en) * 1990-11-29 1992-06-11 The Australian National University Control of plant cell proliferation and growth
US5585542A (en) * 1990-12-21 1996-12-17 Commonwealth Scientific And Industrial Research Organisation DNA sequences encoding at least part of the tomato enzyme endopolygalacturonase PG1 β-subunit
NZ241311A (en) * 1991-01-17 1995-03-28 Gen Hospital Corp Rna sequence having trans-splicing activity, plant strains
NZ314629A (en) * 1991-01-17 2000-08-25 Gen Hospital Corp Use trans-splicing ribozymes to prepare medicaments for gene therapies
GB9109063D0 (en) * 1991-04-26 1991-06-12 Ici Plc Modification of lignin synthesis in plants
GB9112645D0 (en) * 1991-06-12 1991-07-31 Cambridge Advanced Tech Modification of starch production
AU2778992A (en) * 1991-10-07 1993-05-03 Idaho Research Foundation Inc., The Genetic construct for selection of homologous recombinants on a single selective medium
AU667301B2 (en) * 1992-03-24 1996-03-21 Japan Tobacco Inc. Process for reducing seed storage proteins and process for transforming plants
JP2898499B2 (ja) * 1992-03-26 1999-06-02 寳酒造株式会社 エンド型キシログルカン転移酵素遺伝子
US5516694A (en) * 1992-03-26 1996-05-14 Takara Shuzo Co., Ltd. Endo-xyloglucan transferase
JPH07503376A (ja) * 1992-07-30 1995-04-13 ケイヘーネ・エヌ・ベー Ｄｎａ構築物並びにそれから誘導される細胞及び植物
WO1994005795A1 (en) * 1992-08-27 1994-03-17 Commonwealth Scientific And Industrial Research Organisation Dna sequences encoding peach polygalacturonase
CA2148451A1 (en) * 1992-11-02 1994-05-11 Peter Christiaan Sijmons Plants with reduced susceptibility to plant-parasitic nematodes
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US6448474B1 (en) 1995-06-07 2002-09-10 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants
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GB9525353D0 (en) 1995-12-12 1996-02-14 Nat Starch Chem Invest Potato soluble starch synthase
US6075184A (en) * 1996-03-26 2000-06-13 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
JP2001514491A (ja) * 1997-02-21 2001-09-11 ダニスコ エイ／エス デンプン分枝酵素発現のアンチセンスイントロン阻害
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US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
BRPI9908967B1 (pt) 1998-03-20 2017-05-30 Benitec Australia Ltd processos para reprimir, retardar ou de outro modo reduzir a expressão de um gene alvo em uma célula de planta
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US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
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US7019195B1 (en) 1998-05-26 2006-03-28 Syngenta Participations Ag Method for conferring resistance or tolerance aganist furovirus, potyvirus, tospovirus, and cucomovirus to plant cells
AR020078A1 (es) 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
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CA2408326A1 (en) * 2000-06-23 2002-01-03 E.I. Dupont De Nemours And Company Recombinant constructs and their use in reducing gene expression
CN1330753C (zh) 2000-08-30 2007-08-08 北卡罗莱纳州立大学 含有可改变蛋白含量的分子诱导物的转基因植物
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CN1482856A (zh) 2000-11-09 2004-03-17 ����Ƽ���ҵ�о���֯ 降低了ssii活性的大麦和降低了支链淀粉含量的淀粉和淀粉制品
EP1406518A4 (en) 2001-06-08 2006-03-29 Vector Tobacco Ltd CHANGES IN NICOTINE AND NITROSAMINE LEVELS IN TOBACCO
CN1646687A (zh) 2002-03-14 2005-07-27 联邦科学和工业研究组织 修饰的基因沉默rna及其用途
DE10212892A1 (de) 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression
WO2005001098A1 (en) 2003-06-30 2005-01-06 Commonwealth Scientific And Industrial Research Organisation Wheat with altered branching enzyme activity and starch and starch containing products derived thereform
WO2006069422A1 (en) 2004-12-30 2006-07-06 Commonwealth Scientific And Industrial Research Organisation Method and means for improving bowel health
ES2395842T3 (es) 2006-01-17 2013-02-15 Synthon Biopharmaceuticals B.V. Composiciones y métodos para la humanización y optimización de N-glicanos en plantas
EP2436769B1 (en) 2006-06-07 2015-04-01 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Plant expression constructs and methods of utilizing same
US8809626B2 (en) 2007-12-21 2014-08-19 Keygene N.V. Trichome specific promoters
EP2100962A1 (en) 2008-03-12 2009-09-16 Biogemma Plants having improved resistance to pathogens
ES2879599T3 (es) 2009-06-08 2021-11-22 Nunhems Bv Plantas tolerantes a la sequía
NL2004624C2 (en) 2010-04-28 2011-11-01 Stichting Dienst Landbouwkundi A new glycosyltransferase protein and its role in the metabolism of phenylpropanoid volatiles in tomato.
MX344585B (es) 2010-05-28 2016-12-20 Nunhems Bv Platas con un tamaño de fruto incrementado.
NL2014107B1 (en) 2015-01-09 2016-09-29 Limgroup B V New methods and products for breeding of asparagus.
CN108291234B (zh) 2015-09-04 2026-04-21 主基因有限公司 倍数孢子体形成基因
WO2018037123A1 (en) 2016-08-26 2018-03-01 Lesaffre Et Compagnie Improved production of itaconic acid
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EP0477994B1 (en) * 1985-03-21 2001-06-13 Johnston, Stephen, Ph.D. Parasite-derived resistance
US6617496B1 (en) * 1985-10-16 2003-09-09 Monsanto Company Effecting virus resistance in plants through the use of negative strand RNAs
US6608241B1 (en) * 1985-10-29 2003-08-19 Monsanto Technology Llc Protection of plants against viral infection
AU612972B2 (en) * 1986-04-02 1991-07-25 Pioneer Hi-Bred International, Inc. Virus resistant plants having antisense rna
AU611859B2 (en) * 1986-07-25 1991-06-27 Louisiana State University And Agricultural And Mechanical College Method for introduction of disease and pest resistance into plants and novel genes incorporated into plants which code therefor
WO1988001645A1 (en) * 1986-08-26 1988-03-10 Macquarie University Transformation of an organism using antisense rna or promoter occlusion technique
AU7918387A (en) * 1986-08-26 1988-03-24 Macquarie University Transformation of an organism using antisense rna or promoter occlusion technique
GB8626879D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna

Also Published As

Publication number Publication date
JP3670889B2 (ja) 2005-07-13
JPS62296880A (ja) 1987-12-24
ATE207125T1 (de) 2001-11-15
EP0240208A3 (en) 1989-05-03
JP2000023685A (ja) 2000-01-25
DE3752338T2 (de) 2002-06-27
EP0458367B1 (en) 2001-10-17
DE3750755T2 (de) 1995-05-18
AR241938A1 (es) 1993-01-29
EP0240208B1 (en) 1994-11-17
JPH1052283A (ja) 1998-02-24
EP0240208A2 (en) 1987-10-07
DE3752338D1 (de) 2001-11-22
JP2702921B2 (ja) 1998-01-26
IL81737A0 (en) 1987-10-20
GR3015085T3 (en) 1995-05-31
CA1341601C (en) 2010-03-09
DE3750755D1 (de) 1994-12-22
CN87102348A (zh) 1987-12-09
NZ219472A (en) 1990-08-28
AU618234B2 (en) 1991-12-19
AU4447093A (en) 1993-12-09
AU1301792A (en) 1992-09-03
CA1341579C (en) 2008-07-29
ATE114168T1 (de) 1994-12-15
IL81737A (en) 1992-11-15
EP0458367A1 (en) 1991-11-27
AU7059787A (en) 1987-10-01
ES2165348T3 (es) 2002-03-16

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