ES2054608T3 - Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo. - Google Patents
Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo.Info
- Publication number
- ES2054608T3 ES2054608T3 ES86114704T ES86114704T ES2054608T3 ES 2054608 T3 ES2054608 T3 ES 2054608T3 ES 86114704 T ES86114704 T ES 86114704T ES 86114704 T ES86114704 T ES 86114704T ES 2054608 T3 ES2054608 T3 ES 2054608T3
- Authority
- ES
- Spain
- Prior art keywords
- dna fragments
- expression
- sequence
- heme
- segment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012634 fragment Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 2
- 239000013612 plasmid Substances 0.000 title 1
- 230000001737 promoting effect Effects 0.000 abstract 2
- 210000005253 yeast cell Anatomy 0.000 abstract 2
- 238000012270 DNA recombination Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 230000037149 energy metabolism Effects 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 150000003278 haem Chemical class 0.000 abstract 1
- 230000001124 posttranscriptional effect Effects 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000001243 protein synthesis Methods 0.000 abstract 1
- 230000014616 translation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
METODO DE EXPRESION DE GENES EN LEVADURA POR INTRODUCCION EN UNA CELULA DE LEVADURA DE UN SEGMENTO RECOMBINANTE DE DNA QUE CONTIENE EL GEN A EXPRESAR Y UNA REGION DE FLANQUEO 5'' QUE COMPRENDE UNA SECUENCIA PROMOTORA, Y CULTIVO DE LAS CELULAS DE LEVADURA TRANSFORMADOS EN UN MEDIO DE CRECIMIENTO, UTILIZANDO COMO SEGMENTO DE RECOMBINACION DE DNA UN SEGMENTO DEL CUAL, LA REGION DE FLANQUEO 5'' COMPRENDE UN PRIMER FRAGMENTO DE DNA CONTENIENDO UNO, SECUENCIA PROMOTORA EN COMBINACION CON UN SEGUNDO FRAGMENTO DE DNA QUE CONTIENE UNA SECUENCIA CONDUCTORA REGULADA SOBRE EL NIVEL POSTTRANSCRIPCIONAL POR EL HEME, ANALOGOS DEL HEME O PRECURSORES DEL HEME. REALIZANDO EL METODO SEGUN LA INVENCION SE OBTIENE UNA EXPRESION AUMENTADA DE GENES POR REDUCCION DE UNA CARGA GENETICA DE LA CELULA HUESPED Y UNA MEJOR UTILIZACION DEL APARATO DE SINTESIS DE PROTEINAS Y EL METABOLISMO ENERGETICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK488985A DK162900C (da) | 1985-10-24 | 1985-10-24 | Fremgangsmaade til ekspression af gener i gaer samt dna-fragment, rekombineret dna-segment og plasmider, hvor disse indgaar til brug ved udoevelse af fremgangsmaaden |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2054608T3 true ES2054608T3 (es) | 1994-08-16 |
Family
ID=8137569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES86114704T Expired - Lifetime ES2054608T3 (es) | 1985-10-24 | 1986-10-23 | Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US4849348A (es) |
| EP (1) | EP0220679B1 (es) |
| JP (1) | JPS62181787A (es) |
| AT (2) | AT396249B (es) |
| AU (1) | AU593296B2 (es) |
| BR (1) | BR8605221A (es) |
| CA (1) | CA1333162C (es) |
| DE (2) | DE3636117A1 (es) |
| DK (1) | DK162900C (es) |
| ES (1) | ES2054608T3 (es) |
| FI (1) | FI91083C (es) |
| FR (1) | FR2598432B1 (es) |
| GB (1) | GB2183656B (es) |
| IE (1) | IE59114B1 (es) |
| NL (1) | NL8602657A (es) |
| NO (1) | NO175103C (es) |
| NZ (1) | NZ217948A (es) |
| PT (1) | PT83616B (es) |
| SE (1) | SE8604517L (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK162399C (da) * | 1986-01-28 | 1992-03-23 | Danisco | Fremgangsmaade til ekspression af gener i baelgplanteceller, dna-fragment, rekombineret dna-fragment samt plasmid til brug ved udoevelsen af fremgangsmaaden |
| EP0871722A1 (en) * | 1993-05-24 | 1998-10-21 | University Of Massachusetts Medical Center | Post-transcriptional gene regulation by trace minerals |
| US5824511A (en) * | 1995-08-01 | 1998-10-20 | University Technology Corporation | Method for enhancing the production of hemoproteins |
| US6575703B2 (en) | 2001-07-20 | 2003-06-10 | General Electric Company | Turbine disk side plate |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1341302C (en) * | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
-
1985
- 1985-10-24 DK DK488985A patent/DK162900C/da not_active IP Right Cessation
-
1986
- 1986-06-13 US US06/874,069 patent/US4849348A/en not_active Expired - Fee Related
- 1986-10-15 NZ NZ217948A patent/NZ217948A/xx unknown
- 1986-10-21 GB GB8625180A patent/GB2183656B/en not_active Expired - Lifetime
- 1986-10-23 SE SE8604517A patent/SE8604517L/ not_active Application Discontinuation
- 1986-10-23 EP EP86114704A patent/EP0220679B1/en not_active Expired - Lifetime
- 1986-10-23 DE DE19863636117 patent/DE3636117A1/de not_active Ceased
- 1986-10-23 IE IE279386A patent/IE59114B1/en not_active IP Right Cessation
- 1986-10-23 NL NL8602657A patent/NL8602657A/nl not_active Application Discontinuation
- 1986-10-23 FI FI864301A patent/FI91083C/fi not_active IP Right Cessation
- 1986-10-23 AT AT0282386A patent/AT396249B/de not_active IP Right Cessation
- 1986-10-23 NO NO864250A patent/NO175103C/no unknown
- 1986-10-23 ES ES86114704T patent/ES2054608T3/es not_active Expired - Lifetime
- 1986-10-23 AU AU64338/86A patent/AU593296B2/en not_active Ceased
- 1986-10-23 AT AT86114704T patent/ATE90386T1/de active
- 1986-10-23 DE DE8686114704T patent/DE3688549T2/de not_active Expired - Fee Related
- 1986-10-24 PT PT83616A patent/PT83616B/pt not_active IP Right Cessation
- 1986-10-24 FR FR868614807A patent/FR2598432B1/fr not_active Expired
- 1986-10-24 JP JP61253530A patent/JPS62181787A/ja active Pending
- 1986-10-24 CA CA000521391A patent/CA1333162C/en not_active Expired - Fee Related
- 1986-10-24 BR BR8605221A patent/BR8605221A/pt not_active Application Discontinuation
Also Published As
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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