ES2070917T3 - Ensayo a base de cuantificacion de un enzima sobre una mecha. - Google Patents

Ensayo a base de cuantificacion de un enzima sobre una mecha.

Info

Publication number
ES2070917T3
ES2070917T3 ES89313626T ES89313626T ES2070917T3 ES 2070917 T3 ES2070917 T3 ES 2070917T3 ES 89313626 T ES89313626 T ES 89313626T ES 89313626 T ES89313626 T ES 89313626T ES 2070917 T3 ES2070917 T3 ES 2070917T3
Authority
ES
Spain
Prior art keywords
support
fragment
ligando
analytic
conjugate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89313626T
Other languages
English (en)
Inventor
Pyare L Khanna
Glenda L Choate
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microgenics Corp
Original Assignee
Microgenics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microgenics Corp filed Critical Microgenics Corp
Application granted granted Critical
Publication of ES2070917T3 publication Critical patent/ES2070917T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LOS ENSAYOS DIAGNOSTICOS SE PROPORCIONAN COMPRENDIENDO FRAGMENTOS DE ENZIMA COMPLEMENTARIA DE BETA-GALACTOSIDASA, DONDE UNO DE LOS FRAGMENTOS SE LIUMITA A UN APOYO Y EL OTRO FRAGMENTO SE CONJUGA A UN EPITOPO REACTIVO CONTRARIO DEL ANALITICO O COMPLEMENTARIO PARA UN RECEPTOR DE ANALITICO. UNO PUEDE LOGRAR UNA DETERMINACION DE LIGANDO, PROPORCIONANDO LA COMPETENCIA ENTRE EL ANALITICO DE LIGANDO Y EL CONJUGADO PARA RECEPTOR, EN EL QUE LA UNION AL RECEPTOR PERMITE LA DISCRIMINACION ENTRE CONJUGADO DE FRAGMENTO DE ENZIMAS COMPUESTO Y EL CONJUGADO DE FRAGMENTO DE ENZIMA NO COMPUESTO. EL MEDIO DE ENSAYO ES LUEGO PERMITIDO PARA EMPAQUETAR EN UN SOPORTE AL QUE EL FRAGMENTO COMPLEMENTARIO ESTA UNIDO SUSTANCIALMENTE DE MANERA UNIFORME EN LA REGION DE DETECCION. EL SOPORTE ES LUEGO REVELADO, DONDE LA FORMACION DE COLOR DEL SUBSTRATO EN LA REGION DE DETECCION SE UTILIZA COMO UNA MEDIDA DE LA PRESENCIA DEL ANALITICO EN LA MUESTRA.
ES89313626T 1988-12-29 1989-12-28 Ensayo a base de cuantificacion de un enzima sobre una mecha. Expired - Lifetime ES2070917T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29145788A 1988-12-29 1988-12-29

Publications (1)

Publication Number Publication Date
ES2070917T3 true ES2070917T3 (es) 1995-06-16

Family

ID=23120370

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89313626T Expired - Lifetime ES2070917T3 (es) 1988-12-29 1989-12-28 Ensayo a base de cuantificacion de un enzima sobre una mecha.

Country Status (7)

Country Link
EP (1) EP0376707B1 (es)
JP (1) JP2667718B2 (es)
AT (1) ATE118617T1 (es)
AU (1) AU615780B2 (es)
CA (1) CA1336163C (es)
DE (1) DE68921184T2 (es)
ES (1) ES2070917T3 (es)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
AU5280086A (en) * 1985-01-30 1986-08-07 Genetic Diagnostics Corp. Self timed immunoassay device
EP0271204A3 (en) * 1986-11-07 1989-10-25 Syntex (U.S.A.) Inc. Immunochromatographic method and device
WO1989002597A1 (en) * 1987-09-21 1989-03-23 Microgenics Corporation Solid-phase non-separation enzyme assay

Also Published As

Publication number Publication date
JP2667718B2 (ja) 1997-10-27
EP0376707B1 (en) 1995-02-15
EP0376707A3 (en) 1990-10-31
DE68921184D1 (de) 1995-03-23
DE68921184T2 (de) 1995-08-31
EP0376707A2 (en) 1990-07-04
CA1336163C (en) 1995-07-04
ATE118617T1 (de) 1995-03-15
JPH02201265A (ja) 1990-08-09
AU615780B2 (en) 1991-10-10
AU3993089A (en) 1990-07-05

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