ES2072918T3 - Mejoras en la produccion de polipeptidos. - Google Patents

Mejoras en la produccion de polipeptidos.

Info

Publication number
ES2072918T3
ES2072918T3 ES89810335T ES89810335T ES2072918T3 ES 2072918 T3 ES2072918 T3 ES 2072918T3 ES 89810335 T ES89810335 T ES 89810335T ES 89810335 T ES89810335 T ES 89810335T ES 2072918 T3 ES2072918 T3 ES 2072918T3
Authority
ES
Spain
Prior art keywords
production
polypeptides
transformed yeast
refers
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89810335T
Other languages
English (en)
Inventor
Hansjorg Dr Treichler
Kenji Dr Takabayashi
Dieter Heinrich Prof Dr Wolf
Jutta Dr Heim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCP Gen Pharma AG
Novartis AG
Original Assignee
Ciba Geigy AG
UCP Gen Pharma AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888810524A external-priority patent/GB8810524D0/en
Priority claimed from GB888812627A external-priority patent/GB8812627D0/en
Priority claimed from GB898907110A external-priority patent/GB8907110D0/en
Application filed by Ciba Geigy AG, UCP Gen Pharma AG filed Critical Ciba Geigy AG
Application granted granted Critical
Publication of ES2072918T3 publication Critical patent/ES2072918T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Liquid Crystal Substances (AREA)

Abstract

LA INVENCION SE REFIERE A UN NUEVO PROCEDIMIENTO PARA LA PRODUCCIIN DE PROTEINAS HETEROLOGAS, QUE INCLUYE EL EMPLEO DE ESTIRPES DE LEVADURA TRANSFORMADAS DEFICIENTES EN PROTEASA. TAMBIEN SE REFIERE LA INVENCION A DICHAS ESTIRPES DE LEVADURAS TRANSFORMADAS Y A METODOS PARA SU PRODUCCION.
ES89810335T 1988-05-04 1989-05-01 Mejoras en la produccion de polipeptidos. Expired - Lifetime ES2072918T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888810524A GB8810524D0 (en) 1988-05-04 1988-05-04 Improvements in production of polypeptides
GB888812627A GB8812627D0 (en) 1988-05-27 1988-05-27 Improvements in production of polypeptides
GB898907110A GB8907110D0 (en) 1988-05-04 1989-03-29 Improvements in the production of polypeptides

Publications (1)

Publication Number Publication Date
ES2072918T3 true ES2072918T3 (es) 1995-08-01

Family

ID=27263890

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89810335T Expired - Lifetime ES2072918T3 (es) 1988-05-04 1989-05-01 Mejoras en la produccion de polipeptidos.

Country Status (16)

Country Link
EP (1) EP0341215B1 (es)
JP (1) JPH0824565B2 (es)
AT (1) ATE124086T1 (es)
AU (1) AU614121B2 (es)
CA (1) CA1340016C (es)
DE (1) DE68923123T2 (es)
DK (1) DK175701B1 (es)
ES (1) ES2072918T3 (es)
FI (1) FI103129B1 (es)
HU (1) HU218731B (es)
IE (1) IE66044B1 (es)
IL (1) IL90170A (es)
MX (1) MX169508B (es)
NO (1) NO180593C (es)
NZ (1) NZ228955A (es)
PT (1) PT90436B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
WO1991008229A1 (de) * 1989-12-01 1991-06-13 Basf Aktiengesellschaft Hirudinpolyalkylenglykolkonjugate
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
DE69114500T2 (de) * 1990-07-13 1996-06-13 Transkaryotic Therapies Inc Verfahren zum absuchen einer bibliothek.
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
DK0687731T3 (da) 1990-11-08 1999-09-20 Japan Energy Corp Sekretionsvektor, transformerede mikroorganismer indeholdende vektoren og fremstilling af produkter fra denne mikroorganism
SG84484A1 (en) * 1991-02-25 2001-11-20 Ciba Geigy Ag Improved yeast vectors
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
SG49859A1 (en) * 1992-09-04 2002-03-19 Ucp Gen Pharma Ag Process for the production of protease inhibitors
TW282490B (es) * 1992-12-15 1996-08-01 Ciba Geigy Ag
AU685835B2 (en) * 1993-08-04 1998-01-29 Novartis Ag High molecular weight desulphatohirudin
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
US5981227A (en) * 1995-02-09 1999-11-09 Movartis Ag Process for the production of proteins
ES2281079T3 (es) * 1995-03-20 2007-09-16 Novozymes A/S Celula huesped que expresa niveles reducidos de metaloproteasa y metodos de utilizacion de la celula huesped para la produccion de proteinas.
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
AU7112796A (en) * 1995-09-27 1997-04-17 Chiron Corporation Method of making a protease deficient host
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co. Ltd., Tokio/Tokyo Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
FR2768743B1 (fr) * 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
AU2018348518B2 (en) 2017-10-10 2024-05-16 Ajinomoto Co., Inc. Method for manufacturing protein
EP4166647A4 (en) 2020-06-11 2025-01-01 Ajinomoto Co., Inc. Method for producing protein

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
JPS62224284A (ja) * 1986-03-25 1987-10-02 Agency Of Ind Science & Technol 微生物
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins

Also Published As

Publication number Publication date
MX169508B (es) 1993-07-08
CA1340016C (en) 1998-08-25
DK217589D0 (da) 1989-05-03
AU3382889A (en) 1989-11-09
IL90170A (en) 1995-12-31
JPH02104279A (ja) 1990-04-17
JPH0824565B2 (ja) 1996-03-13
NZ228955A (en) 1991-06-25
IE66044B1 (en) 1995-12-13
FI892114L (fi) 1989-11-05
FI892114A0 (fi) 1989-05-03
PT90436A (pt) 1989-11-30
EP0341215A2 (en) 1989-11-08
IL90170A0 (en) 1989-12-15
NO180593C (no) 1997-05-14
AU614121B2 (en) 1991-08-22
ATE124086T1 (de) 1995-07-15
NO891837D0 (no) 1989-05-03
EP0341215A3 (en) 1992-01-15
DE68923123T2 (de) 1995-11-30
EP0341215B1 (en) 1995-06-21
HU218731B (hu) 2000-11-28
DK217589A (da) 1989-11-05
IE891451L (en) 1989-11-04
FI103129B (fi) 1999-04-30
DK175701B1 (da) 2005-01-24
DE68923123D1 (de) 1995-07-27
PT90436B (pt) 1994-11-30
NO891837L (no) 1989-11-06
NO180593B (no) 1997-02-03
HUT54210A (en) 1991-01-28
FI103129B1 (fi) 1999-04-30

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