ES2073491T3 - Arn multifuncional con actividad de autoprocesamiento, su preparacion y su utilizacion. - Google Patents

Arn multifuncional con actividad de autoprocesamiento, su preparacion y su utilizacion.

Info

Publication number
ES2073491T3
ES2073491T3 ES90118938T ES90118938T ES2073491T3 ES 2073491 T3 ES2073491 T3 ES 2073491T3 ES 90118938 T ES90118938 T ES 90118938T ES 90118938 T ES90118938 T ES 90118938T ES 2073491 T3 ES2073491 T3 ES 2073491T3
Authority
ES
Spain
Prior art keywords
rna
self
preparation
processing activity
multifunctional rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90118938T
Other languages
English (en)
Inventor
Hubert Dr Mullner
Eugen Dr Uhlmann
Peter Dr Eckes
Rudolf Dr Schneider
Bernardus Dr Uijtewaal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience AG
Original Assignee
Hoechst Schering Agrevo GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Schering Agrevo GmbH filed Critical Hoechst Schering Agrevo GmbH
Application granted granted Critical
Publication of ES2073491T3 publication Critical patent/ES2073491T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL INVENTO SE REFIERE A UNA RNA RIBOCIMA, QUE SE EXPRESAN COMO RNA ANTIDESTRUCCION, SE COMBINA CON OTRAS RNA POR UN ESPACIADOR, SE COMPLEMENTA CON RNA VIRUS DETERMINADO Y SE APLICA EN PLANTAS QUE SON TRANSFORMADAS CON GENES CODIFICADORES DE RNA Y QUE PRESENTA UNA ACCION MEJORADA CONTRA LOS VIRUS.
ES90118938T 1989-10-06 1990-10-04 Arn multifuncional con actividad de autoprocesamiento, su preparacion y su utilizacion. Expired - Lifetime ES2073491T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3933384A DE3933384A1 (de) 1989-10-06 1989-10-06 Multifunktionelle rna mit selbstprozessierungsaktivitaet, ihre herstellung und ihre verwendung

Publications (1)

Publication Number Publication Date
ES2073491T3 true ES2073491T3 (es) 1995-08-16

Family

ID=6390949

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90118938T Expired - Lifetime ES2073491T3 (es) 1989-10-06 1990-10-04 Arn multifuncional con actividad de autoprocesamiento, su preparacion y su utilizacion.

Country Status (12)

Country Link
US (2) US5707840A (es)
EP (1) EP0421376B1 (es)
JP (1) JPH03210180A (es)
KR (1) KR910008132A (es)
AT (1) ATE121452T1 (es)
AU (1) AU634289B2 (es)
DE (2) DE3933384A1 (es)
DK (1) DK0421376T3 (es)
ES (1) ES2073491T3 (es)
HU (1) HUT62038A (es)
IE (1) IE67652B1 (es)
NZ (1) NZ235562A (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4002885A1 (de) * 1990-02-01 1991-08-08 Hoechst Ag Expression einer multigen rna mit self-splicing aktivitaet
US5633449A (en) * 1990-03-12 1997-05-27 Cornell Research Foundation, Inc. Induction of resistance to viral diseases in plants
CA2078134A1 (en) * 1990-03-12 1991-09-13 Milton Zaitlin Transformation of plants with non-structural plant virus gene sequences
US5596132A (en) * 1990-03-12 1997-01-21 Cornell Research Foundation, Inc. Induction of resistance to virus diseases by transformation of plants with a portion of a plant virus genome involving a read-through replicase gene
GB9018646D0 (en) * 1990-08-24 1990-10-10 Imperial College Replication of a eukaryotic virus rna
DK0625194T3 (da) * 1992-02-04 1996-05-28 Worcester Found Ex Biology Forøgelse af ribozym-katalytisk aktivitet med et tilstødende facilitator oligonukleotid
US6087484A (en) * 1992-02-04 2000-07-11 University Of Massachusetts Worcester Enhancement of ribozyme catalytic activity by A 2'-O-substituted facilitator oligonucleotide
KR950700408A (ko) * 1992-02-04 1995-01-16 토루페터슨 인접한 촉진제 올리고누클레오티드에 의한 리보자임 촉매활성의 증강(enhancement of ribozyme catalytic activity by a neighboring facilitator oligonucleotide)
DE4203441C1 (de) * 1992-02-06 1993-10-14 Max Planck Gesellschaft RNA- und DNA-Moleküle zur Erzeugung von Virusresistenzen
AU656676B2 (en) * 1992-04-17 1995-02-09 Kirin Beer Kabushiki Kaisha Plant resistant to two or more viruses and preparation thereof
ATE312510T1 (de) * 1992-06-29 2005-12-15 Gene Shears Pty Ltd Nukleinsäuren und sie verwendende verfahren zur bekämpfung viraler pathogene
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias
FR2701960B1 (fr) * 1993-02-26 2002-09-13 Gene Shears Pty Ltd Polyribozyme apte à conférer, aux plantes, une résistance aux virus et plantes résistantes produisant ce polyribozyme.
JPH09505461A (ja) * 1993-07-22 1997-06-03 ジーン シェアーズ プロプライアタリー リミティド Ｄｎａウィルスリボザイム
GB9320548D0 (en) * 1993-10-06 1993-11-24 Sandoz Ltd Improvements in or relating to organic compounds
US5908779A (en) * 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
DE4420298A1 (de) * 1994-06-10 1995-12-14 Hoechst Schering Agrevo Gmbh Modifizierte Satelliten-RNAs und Satelliten-Viren als Träger adaptierter Ribozyme
US5824519A (en) * 1995-11-08 1998-10-20 Medical University Of South Carolina Tissue-specific and target RNA-specific ribozymes
IT1283631B1 (it) * 1996-05-09 1998-04-23 Metapontum Agrobios Srl Metodo per la preparazione di piante transgeniche resistenti ad in- fezioni virali e piante cosi' ottenute
DE10212892A1 (de) * 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
AU632993B2 (en) * 1987-12-15 1993-01-21 Gene Shears Pty. Limited Ribozymes
DK0625194T3 (da) * 1992-02-04 1996-05-28 Worcester Found Ex Biology Forøgelse af ribozym-katalytisk aktivitet med et tilstødende facilitator oligonukleotid

Also Published As

Publication number Publication date
EP0421376B1 (de) 1995-04-19
NZ235562A (en) 1992-12-23
IE903579A1 (en) 1991-04-10
IE67652B1 (en) 1996-04-17
ATE121452T1 (de) 1995-05-15
JPH03210180A (ja) 1991-09-13
DK0421376T3 (da) 1995-09-04
HUT62038A (en) 1993-03-29
HU906339D0 (en) 1991-04-29
AU634289B2 (en) 1993-02-18
EP0421376A1 (de) 1991-04-10
DE3933384A1 (de) 1991-04-18
US5834265A (en) 1998-11-10
AU6382790A (en) 1991-04-11
DE59008928D1 (de) 1995-05-24
KR910008132A (ko) 1991-05-30
US5707840A (en) 1998-01-13

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