ES2074887T3 - Mutaciones de trpr eficaces en la expresion de proteinas y peptidos pequeños. - Google Patents
Mutaciones de trpr eficaces en la expresion de proteinas y peptidos pequeños.Info
- Publication number
- ES2074887T3 ES2074887T3 ES92910290T ES92910290T ES2074887T3 ES 2074887 T3 ES2074887 T3 ES 2074887T3 ES 92910290 T ES92910290 T ES 92910290T ES 92910290 T ES92910290 T ES 92910290T ES 2074887 T3 ES2074887 T3 ES 2074887T3
- Authority
- ES
- Spain
- Prior art keywords
- lms
- expression
- trpr
- mutations
- peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 230000035772 mutation Effects 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 244000005700 microbiome Species 0.000 abstract 2
- 108700034853 E coli TRPR Proteins 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 101150006320 trpR gene Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
ESTA INVENCION PROPORCIONA MICROORGANISMOS NUEVOS QUE TIENEN UNA PROTEINA (TRPR) REPRESORA DE TRIPTOFAN INACTIVA. LOS MICROROGANISMOS PREFERENTES, "E. COLI STRAINS" LMS 117, Y LMS ROTEINAS CUANDO SE TRANSFORMAN CON VECTORES ADECUADOS DE EXPRESION. TAMBIEN SE DESCRIBE UN "PLASMID" PRALI, QUE, CUANDO SE TRANSFORMA EN UN HUESPED ADECUADO, PREFERENTEMENTE LMS LMS EINAS. TAMBIEN SE DESCRIBE UN METODO PARA AUMENTAR LA EXPRESION DE LAS PROTEINAS MEDIANTE LA INACTIVACION DEL CONTROL REPRESOR "OPERON" TRIPTOFANO.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68168891A | 1991-04-05 | 1991-04-05 | |
| US83731392A | 1992-02-18 | 1992-02-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2074887T3 true ES2074887T3 (es) | 1995-09-16 |
Family
ID=27102710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92910290T Expired - Lifetime ES2074887T3 (es) | 1991-04-05 | 1992-03-27 | Mutaciones de trpr eficaces en la expresion de proteinas y peptidos pequeños. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0578754B1 (es) |
| JP (1) | JPH06506118A (es) |
| AT (1) | ATE123527T1 (es) |
| AU (1) | AU1753092A (es) |
| DE (1) | DE69202859T2 (es) |
| DK (1) | DK0578754T3 (es) |
| ES (1) | ES2074887T3 (es) |
| WO (1) | WO1992017594A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10504971A (ja) * | 1994-09-08 | 1998-05-19 | カイロン コーポレイション | インスリン様増殖因子の改善された生産方法 |
| GB2304718B (en) * | 1995-09-05 | 2000-01-19 | Degussa | The production of tryptophan by the bacterium escherichia coli |
| EP0966521A1 (en) * | 1996-09-13 | 1999-12-29 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK108685A (da) * | 1984-03-19 | 1985-09-20 | Fujisawa Pharmaceutical Co | Vaekstfaktor i |
| CA1338664C (en) * | 1985-04-10 | 1996-10-22 | Cetus Oncology Corporation | Recombinant hosts |
-
1992
- 1992-03-27 EP EP92910290A patent/EP0578754B1/en not_active Expired - Lifetime
- 1992-03-27 WO PCT/US1992/002235 patent/WO1992017594A1/en not_active Ceased
- 1992-03-27 AT AT92910290T patent/ATE123527T1/de not_active IP Right Cessation
- 1992-03-27 DE DE69202859T patent/DE69202859T2/de not_active Expired - Fee Related
- 1992-03-27 JP JP4509432A patent/JPH06506118A/ja active Pending
- 1992-03-27 DK DK92910290.3T patent/DK0578754T3/da active
- 1992-03-27 ES ES92910290T patent/ES2074887T3/es not_active Expired - Lifetime
- 1992-03-27 AU AU17530/92A patent/AU1753092A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE69202859D1 (de) | 1995-07-13 |
| EP0578754A1 (en) | 1994-01-19 |
| JPH06506118A (ja) | 1994-07-14 |
| EP0578754B1 (en) | 1995-06-07 |
| ATE123527T1 (de) | 1995-06-15 |
| WO1992017594A1 (en) | 1992-10-15 |
| AU1753092A (en) | 1992-11-02 |
| DE69202859T2 (de) | 1995-12-07 |
| DK0578754T3 (da) | 1995-11-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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