ES2074935A1 - Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion. - Google Patents

Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion.

Info

Publication number
ES2074935A1
ES2074935A1 ES09300408A ES9300408A ES2074935A1 ES 2074935 A1 ES2074935 A1 ES 2074935A1 ES 09300408 A ES09300408 A ES 09300408A ES 9300408 A ES9300408 A ES 9300408A ES 2074935 A1 ES2074935 A1 ES 2074935A1
Authority
ES
Spain
Prior art keywords
protein
ebulin
plant
obtaining
sambucus ebulus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09300408A
Other languages
English (en)
Other versions
ES2074935B1 (es
Inventor
Juan Tomas Girbes
Vallejo Francisco J Arias
Rodriguez Mari Angeles Rojo
Alvarez Rosario Iglesias
Rodriguez Jose Miguel Ferreras
Gonzalez Lucia Citores
Martinez Raquel Munoz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Valladolid
Original Assignee
Universidad de Valladolid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES9300408A priority Critical patent/ES2074935B1/es
Application filed by Universidad de Valladolid filed Critical Universidad de Valladolid
Priority to JP6519616A priority patent/JPH07506842A/ja
Priority to PCT/ES1994/000020 priority patent/WO1994020540A1/es
Priority to EP94909123A priority patent/EP0652231A1/en
Priority to ZA941461A priority patent/ZA941461B/xx
Priority to AU62084/94A priority patent/AU6208494A/en
Publication of ES2074935A1 publication Critical patent/ES2074935A1/es
Application granted granted Critical
Publication of ES2074935B1 publication Critical patent/ES2074935B1/es
Priority to US08/890,129 priority patent/US6008324A/en
Priority to JP2003427447A priority patent/JP2004113252A/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

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  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROTEINA EBULINA 1 DE LA PLANTA SAMBUCUS EBULUS L., PROCEDIMIENTO PARA SU OBTENCION Y SU UTILIZACION. LA EBULINA 1 ES UNA PROTEINA VEGETAL INACTIVADORA DE RIBOSOMAS DEL TIPO DE DOS CADENA A Y B CON UNA MASA MOLECULAR RELATIVA DE 26.000 Y 30.200 RESPECTIVAMENTE. EL PROCEDIMIENTO COMPRENDE: EXTRAER LA PLANTA SAMBUCUS EBULUS L., FILTRAR EL EXTRACTO LIQUIDO Y CENTRIFUGARLO; SOMETER EL FLUIDO SOBRENADANTE A CROMATOGRAFIA DE AFINIDAD RECOGIENDO LA FRACCION PROTEINICA LA CUAL SE CONCENTRA Y SE SOMETE A OTRA CROMATOGRAFIA DE AFINIDAD PARA OBTENER LA PROTEINA DESEADA. LA EBULINA 1 SE UTILIZA COMO INACTIVADOR DEL ACIDO RIBONUCLEICO E INHIBIDOR DE LA SINTESIS DE PROTEINAS.
ES9300408A 1993-03-02 1993-03-02 Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion Expired - Fee Related ES2074935B1 (es)

Priority Applications (8)

Application Number Priority Date Filing Date Title
ES9300408A ES2074935B1 (es) 1993-03-02 1993-03-02 Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion
PCT/ES1994/000020 WO1994020540A1 (es) 1993-03-02 1994-03-02 PROTEINAS INACTIVADORAS DE RIBOSOMAS (RIPs) DE DOS CADENAS NO TOXICAS, PROCEDIMIENTO PARA SU PREPARACION Y APLICACIONES
EP94909123A EP0652231A1 (en) 1993-03-02 1994-03-02 NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS
ZA941461A ZA941461B (en) 1993-03-02 1994-03-02 Non-toxic two-chain ribosome-inactivating proteins (rips) process for the preparation and uses thereof
JP6519616A JPH07506842A (ja) 1993-03-02 1994-03-02 二本鎖を有する非毒性リボソーム不活性化蛋白（ｒｉｐ），その製法および適用
AU62084/94A AU6208494A (en) 1993-03-02 1994-03-02 Non-toxic ribosome inactivating proteins (rips) with two chains, process for the preparation thereof and applications
US08/890,129 US6008324A (en) 1993-03-02 1997-07-09 Non-toxic ribosome inactivating proteins (RIPs) with two chains, process for the preparation thereof and applications
JP2003427447A JP2004113252A (ja) 1993-03-02 2003-12-24 二本鎖を有する非毒性リボソーム不活性化蛋白、その製法および調製物

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9300408A ES2074935B1 (es) 1993-03-02 1993-03-02 Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion

Publications (2)

Publication Number Publication Date
ES2074935A1 true ES2074935A1 (es) 1995-09-16
ES2074935B1 ES2074935B1 (es) 1996-05-16

Family

ID=8280952

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9300408A Expired - Fee Related ES2074935B1 (es) 1993-03-02 1993-03-02 Proteina ebulina 1 de la planta sambucus ebulus l., procedimiento para su obtencion y su utilizacion

Country Status (2)

Country Link
ES (1) ES2074935B1 (es)
ZA (1) ZA941461B (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2169968A1 (es) * 1999-08-25 2002-07-16 Univ Valladolid Gen que codifica la proteina inactivadora de ribosomas de hojas de sambucus ebulus l. procedimiento para su obtencion y aplicaciones.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0390040A1 (en) * 1989-03-31 1990-10-03 ITALFARMACO S.p.A. Ribosome inactivating proteins and derivatives thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0390040A1 (en) * 1989-03-31 1990-10-03 ITALFARMACO S.p.A. Ribosome inactivating proteins and derivatives thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF EXPERIMENTAL BOTANY. Enero 1990. Vol. 41, n‘ 222. pßg. 67-70. M.J. MERINO et al. "Plant Species Containing Inhibitors of Eukaryotic Polypeptide Synthesis" * Todo el documento * *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2169968A1 (es) * 1999-08-25 2002-07-16 Univ Valladolid Gen que codifica la proteina inactivadora de ribosomas de hojas de sambucus ebulus l. procedimiento para su obtencion y aplicaciones.

Also Published As

Publication number Publication date
ES2074935B1 (es) 1996-05-16
ZA941461B (en) 1994-09-27

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Effective date: 20141120