ES2075802B1 - Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento". - Google Patents

Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento".

Info

Publication number
ES2075802B1
ES2075802B1 ES9302526A ES9302526A ES2075802B1 ES 2075802 B1 ES2075802 B1 ES 2075802B1 ES 9302526 A ES9302526 A ES 9302526A ES 9302526 A ES9302526 A ES 9302526A ES 2075802 B1 ES2075802 B1 ES 2075802B1
Authority
ES
Spain
Prior art keywords
elisa
atroseptica
enrichment
procedure
eca
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9302526A
Other languages
English (en)
Other versions
ES2075802A1 (es
Inventor
Mariano Cambra
Maria Teresa Gorris
M Milagros Lopez
Benito Alarcon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES9302526A priority Critical patent/ES2075802B1/es
Priority to GB9424387A priority patent/GB2285863B/en
Priority to NL9402018A priority patent/NL193597C/nl
Publication of ES2075802A1 publication Critical patent/ES2075802A1/es
Application granted granted Critical
Publication of ES2075802B1 publication Critical patent/ES2075802B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Gram-negative bacteria
    • C07K16/1228—Enterobacterales (O), e.g. Citrobacter (G), Serratia (G), Proteus (G), Providencia (G), Morganella (G) or Yersinia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA LA DETECCION SENSIBLE Y ESPECIFICA DE ERWINIA CAROTOVORA SUBSP. ATROSEPTICA MEDIANTE "ELISA-ENRIQUECIMIENTO". EL PROCEDIMIENTO PARTE DE LA INSOLUBILIZACION DE ANTIGENOS DEL AGENTE PATOGENO ERWINIA CAROTOVORA SUBSP. ATROSEPTICA (ECA) EN MICROPLACAS, MEDIANTE ANTICUERPOS MONOCLONALES O POLICLONALES. POSTERIORMENTE, EN LA MISMA MICROPLACA, SE AÑADE LA MUESTRA OBJETO DE ESTUDIO ADICIONADA CON UN MEDIO SELECTIVO PARA ERWINIAS (D-PEM) Y SE INCUBA EN ANAEROBIOSIS A 25 C DURANTE 24-48 HORAS. LA REACCION SE REVELA MEDIANTE ANTICUERPOS MONOCLONALES ESPECIFICOS DE ECA (4G4) BIOTINILADOS Y POSTERIOR ADICION DE ESTREPTOAVIDINA MARCADA CON UNA ENZIMA O DIRECTAMENTE CON ANTICUERPOS 4G4 MARCADOS CON UNA ENZIMA (ELISA-DAS O DAS BIOTINA/ESTREPTOAVIDINA) O CON ANTICUERPOS 4G4 SIN MARCAR Y POSTERIOR ADICION DE UN CONJUGADO ANTI-ESPECIE (ELISA-DASI). LA HIDROLISIS DEL SUBSTRATO ENZIMATICO IMPLICA LA APARICION DE UN PRODUCTO COLOREADO QUE ES VALORADO FOTOCOLORIMETRICAMENTE. LA REALIZACION PARALELA DE LA MUESTRA EN MICROPLACAS ENRIQUECIDAS CON D-PEN O SIN ENRIQUECER, PERMITE CONCLUIR SOBRE LA PRESENCIA Y CANTIDAD DE CELULAS VIVAS DE ECA CAPACES DE PROVOCAR ENFERMEDAD EN TUBERCULOS U OTRO MATERIAL VEGETAL.
ES9302526A 1993-12-02 1993-12-02 Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento". Expired - Fee Related ES2075802B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9302526A ES2075802B1 (es) 1993-12-02 1993-12-02 Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento".
GB9424387A GB2285863B (en) 1993-12-02 1994-12-01 Method for the detection of Erwinia carotovora atroseptica subspecies by "ELISA-Enrichment"
NL9402018A NL193597C (nl) 1993-12-02 1994-12-01 Voor Erwinia carotovora subsp. atroseptica (Eca) specifiek monoklonaal antilichaam, en werkwijze voor een gevoelige en specifieke detectie van Erwinia carotovora subsp. atroseptica (Eca) onder toepassing van öELISA-verrijkingö.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9302526A ES2075802B1 (es) 1993-12-02 1993-12-02 Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento".

Publications (2)

Publication Number Publication Date
ES2075802A1 ES2075802A1 (es) 1995-10-01
ES2075802B1 true ES2075802B1 (es) 1996-05-01

Family

ID=8283811

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9302526A Expired - Fee Related ES2075802B1 (es) 1993-12-02 1993-12-02 Procedimiento para la deteccion sensible y especifica de erwinia carotovora subsp. atroseptica mediante "elisa-enriquecimiento".

Country Status (3)

Country Link
ES (1) ES2075802B1 (es)
GB (1) GB2285863B (es)
NL (1) NL193597C (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010229924B2 (en) 2009-03-24 2016-07-21 Plus Therapeutics Inc. Devices and methods of cell capture and analysis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0363571A (ja) * 1989-08-02 1991-03-19 Chisso Corp 大腸菌群の検査キット
EP0533772A4 (en) * 1990-06-08 1993-12-08 Biotech Australia Pty. Limited Detection process
FR2674866A1 (fr) * 1991-04-08 1992-10-09 Chemunex Anticorps anti-ace et leurs applications a la detection specifique et eventuellement a la numeration des enterobacteries entieres, par une methode immunochimique.

Also Published As

Publication number Publication date
NL193597C (nl) 2000-03-02
GB2285863B (en) 1998-01-28
ES2075802A1 (es) 1995-10-01
NL9402018A (nl) 1995-07-03
GB2285863A (en) 1995-07-26
NL193597B (nl) 1999-11-01
GB9424387D0 (en) 1995-01-18

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Effective date: 20071203