ES2078174A1 - Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19. - Google Patents
Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19.Info
- Publication number
- ES2078174A1 ES2078174A1 ES09302728A ES9302728A ES2078174A1 ES 2078174 A1 ES2078174 A1 ES 2078174A1 ES 09302728 A ES09302728 A ES 09302728A ES 9302728 A ES9302728 A ES 9302728A ES 2078174 A1 ES2078174 A1 ES 2078174A1
- Authority
- ES
- Spain
- Prior art keywords
- cvabrucella
- differentiation
- methods
- molecular diagnostic
- diagnostic methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002405 diagnostic procedure Methods 0.000 title abstract 3
- 230000004069 differentiation Effects 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 241000589567 Brucella abortus Species 0.000 title 1
- 229940056450 brucella abortus Drugs 0.000 title 1
- 238000000034 method Methods 0.000 abstract 5
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 abstract 1
- 239000004386 Erythritol Substances 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 abstract 1
- 229940009714 erythritol Drugs 0.000 abstract 1
- 235000019414 erythritol Nutrition 0.000 abstract 1
- 238000013412 genome amplification Methods 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODOS DE DIAGNOSTICO MOLECULAR PARA LA DETECCION DE CVABRUCELLA ABORTUS Y SU DIFERENCIACION DE LA CEPA VACUNAL B19. SE DESCRIBEN UNA SERIE DE METODOS DE DIAGNOSTICO MOLECULAR QUE PERMITEN A DIFERENCIACION ENTRE LA ESTIRPE VACUNAL CVABRUCELLA ABORTUS B19 Y TODAS LAS DEMAS ESTIRPES DEL GENERO CVABRUCELLA. EN UN CASO SE APLICA LA AMPLIFICACION DE GENOMA, USANDO COMO INICIADORES OLIGONUCLEOTIDOS DERIVADOS DE LA REGION QUE CONTIENE LOS GENES CODIFICANTES DE LA RUTA DE UTILIZACION DEL ERITRITOL. OTRO GRUPO DE METODOS SE BASA EN LA HIBRIDACION DE ADN GENOMICO USANDO COMO SONDAS OLIGONUCLEOTIDOS DE LA MENCIONADA REGION CVAERI. EN ESTE CASO PUEDEN APLICARSE SEGUN LA TECNICA DENOMINADA "HIBRIDACION TIPO SOUTHERN" O ALTERNATIVAMENTE POR EL PROCEDIMIENTO DEL "DOT BLOT". FINALMENTE SE APLICAN ESTOS METODOS PARA LA IDENTIFICACION DE GERMENES DEL GENERO CVABRUCELLA EN CUALQUIER MUESTRA BIOLOGICA. LOS NUEVOS PROCEDIMIENTOS SON VENTAJOSOS SOBRE LOS METODOS TRADICIONALES, INMUNOLOGICOS O DE CULTIVO, APORTANDO MAYOR ESPECIFICIDAD Y RAPIDEZ.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09302728A ES2078174B1 (es) | 1993-12-16 | 1993-12-16 | Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09302728A ES2078174B1 (es) | 1993-12-16 | 1993-12-16 | Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2078174A1 true ES2078174A1 (es) | 1995-12-01 |
| ES2078174B1 ES2078174B1 (es) | 1996-08-16 |
Family
ID=8284078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09302728A Expired - Fee Related ES2078174B1 (es) | 1993-12-16 | 1993-12-16 | Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2078174B1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097347A3 (en) * | 2005-03-17 | 2007-01-04 | Inst Cientifico Tecnol Navarra | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
-
1993
- 1993-12-16 ES ES09302728A patent/ES2078174B1/es not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| US4358535B1 (es) * | 1980-12-08 | 1986-05-13 | ||
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683202B1 (es) * | 1985-03-28 | 1990-11-27 | Cetus Corp |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097347A3 (en) * | 2005-03-17 | 2007-01-04 | Inst Cientifico Tecnol Navarra | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2078174B1 (es) | 1996-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PT1037909E (pt) | Síndrome multi-sistémica do definhamento pós-desmame de porcos | |
| Kostman et al. | A universal approach to bacterial molecular epidemiology by polymerase chain reaction ribotyping | |
| ES2315215T1 (es) | Secuencias nucleotidicas del genoma de retrovirus del tipo hiv-1, hiv-2, y siv y sus aplicaciones, en particular para la amplificacion de genomas de estos retrovirus y para el diagnostico in vitro de infecciones debidas a estos virus. | |
| AR035215A1 (es) | Polinucleotido aislado, primer y segundo polinucleotido cebador, metodo para detectar el suceso vegetal de algodon 531, molecula de polinucleotido aislado obtenida por dicho metodo, equipo de deteccion de acido nucleico y metodo para determinar la cigosidad del genoma de una planta de algodon. | |
| ES2805874T3 (es) | Ligación enzimática de ácidos nucleicos | |
| Cabrera et al. | Identity tests: determination of cell line cross-contamination | |
| BR9814912A (pt) | Sequência genÈmica e polipeptìdeos de chlamydia trachomatis, fragmentos dos mesmos e usos dos mesmos, em particular para o diagnóstico, a prevenção e o tratamento de infecção | |
| ES8606903A1 (es) | Un metodo para entregar un agente seleccionado a un lugar biologico caracterizado por la presencia de una secuencia de adn seleccionada. | |
| AR040710A1 (es) | Evento de maiz pv-zmir 13 (mon863), plantas, composiciones y metodos para la deteccion del mismo | |
| ES2375406T3 (es) | Sonda y cebador para la detección de bacilos tuberculosos, y procedimiento para la detección de bacilos tuberculosos humanos con el uso de los mismos. | |
| ES2056366T3 (es) | Dna-polimerasa de t7. | |
| Bosi et al. | Serratia marcescens nosocomial outbreak due to contamination of hexetidine solution | |
| AR022301A1 (es) | Marcadores de adn microsatelite y uso de los mismos | |
| Chen et al. | Identification by random amplification of polymorphic DNA of a common molecular type of Cryptococcus neoformans var. neoformans in patients with AIDS or other immunosuppressive conditions | |
| AU2005333512A1 (en) | Nucleic acid detection method involving the direct generation of a measurable signal | |
| ES2327593B1 (es) | Metodo de identificacion de especies bacterianas de los generos anaplasma/ehrlichia y bartonella. | |
| Doronin et al. | Photoaffinity labeling of DNA polymerase α DNA primase complex based on the catalytic competence of a dNTP reactive analog | |
| KR930008138A (ko) | 특정한 뉴클레오티드 시퀀스를 포함하는 약독화(弱毒化)된 홍역 백신 바이러스 균주와 그의 완벽한 동정 방법 | |
| ES2286212T3 (es) | Secuencias suprimidas especificamente del genoma de mycobacterium tuberculosis, y su uso en el diagnostico y como vacunas. | |
| MIZUKAMI | Amplification and sequence of a 5s-rRNA gene spacer region from the crude drug" angelica root" | |
| ES2078174A1 (es) | Metodo de diagnostico molecular para la deteccion de brucella abortus y en diferenciacion de la cepa vacunal b19. | |
| RU2360971C1 (ru) | Способ и тест-система для обнаружения днк вируса африканской чумы свиней с помощью специфических олигонуклеотидных праймеров в полимеразной цепной реакции | |
| ES2643214T3 (es) | Procedimiento de detección para Mycobacterium avium ssp. paratuberculosis | |
| Ménard et al. | Randomly amplified polymorphic DNA analysis confirms the biotyping scheme of Porphyromonas gingivalis | |
| ES2048652B1 (es) | Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 19931216 Kind code of ref document: B1 Effective date: 19931216 |
|
| TK9A | Correction |
Free format text: EN EL BOPI 011295 SE PUBLICA LA TEXTO DEL IET DEL EXPE0 DIENTE CON ERROR EN:TEXTO DEL IET 0 |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20141120 |