ES2078327T3 - Reactivo y metodo de utilizacion del mismo para la determinacion automatica en citometria de flujo de al menos una sub-poblacion leucocitaria a partir de la sangre total. - Google Patents

Reactivo y metodo de utilizacion del mismo para la determinacion automatica en citometria de flujo de al menos una sub-poblacion leucocitaria a partir de la sangre total.

Info

Publication number
ES2078327T3
ES2078327T3 ES90403231T ES90403231T ES2078327T3 ES 2078327 T3 ES2078327 T3 ES 2078327T3 ES 90403231 T ES90403231 T ES 90403231T ES 90403231 T ES90403231 T ES 90403231T ES 2078327 T3 ES2078327 T3 ES 2078327T3
Authority
ES
Spain
Prior art keywords
reagent
sub
leukocytary
population
flow cytometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90403231T
Other languages
English (en)
Inventor
Didier Lefevre
Henri Champseix
Nadine Voltas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ABX SA
Original Assignee
ABX SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ABX SA filed Critical ABX SA
Application granted granted Critical
Publication of ES2078327T3 publication Critical patent/ES2078327T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN REACTIVO Y A UN METODO DE UTILIZACION DEL MISMO PARA LA DETERMINACION AUTOMATICA DE AL MENOS UNA SUB-POBLACION LEUCOCITARIA DE UNA MUESTRA DE SANGRE TOTAL. ESTE REACTIVO PERMITE, EN UNA SOLA ETAPA, DESTRUIR LOS ERITROCITOS POR LA ACCION DE LA SAPONINA Y DEL SDS Y ASEGURAR UN MARCAJE DIFERENCIAL DE LAS SUB-POBLACIONES LEUCOCITARIAS AL MISMO TIEMPO QUE SE PRESERVAN LA INTEGRIDAD Y LA MORFOLOGIA CELULARES, POR LA ACCION COMBINADA DE DIVERSOS CONSTITUYENTES QUE COMPRENDEN EL NEGRO CLORAZOL, UN ALCOHOL, UN AGENTE TENSOACTIVO, PRESERVANTES. EL REACTIVO DE LA INVENCION ENCUENTRA SU APLICACION EN LOS DIFERENTES TIPOS DE APARATOS DE CONTADO AUTOMATICO DE CITOMETRIA DE FLUJO.
ES90403231T 1989-11-20 1990-11-15 Reactivo y metodo de utilizacion del mismo para la determinacion automatica en citometria de flujo de al menos una sub-poblacion leucocitaria a partir de la sangre total. Expired - Lifetime ES2078327T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8915166A FR2654744B1 (fr) 1989-11-20 1989-11-20 Reactif et methode d'utilisation de celui-ci pour la determination automatique en cytometrie de flux d'au moins une sous-population leucocytaire a partir du sang total.

Publications (1)

Publication Number Publication Date
ES2078327T3 true ES2078327T3 (es) 1995-12-16

Family

ID=9387538

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90403231T Expired - Lifetime ES2078327T3 (es) 1989-11-20 1990-11-15 Reactivo y metodo de utilizacion del mismo para la determinacion automatica en citometria de flujo de al menos una sub-poblacion leucocitaria a partir de la sangre total.

Country Status (17)

Country Link
US (1) US5232857A (es)
EP (1) EP0430750B1 (es)
JP (1) JP2920690B2 (es)
KR (1) KR100194407B1 (es)
AT (1) ATE117803T1 (es)
AU (1) AU634600B2 (es)
CA (1) CA2030381C (es)
DE (1) DE69016377T2 (es)
DK (1) DK0430750T3 (es)
ES (1) ES2078327T3 (es)
FI (1) FI93658C (es)
FR (1) FR2654744B1 (es)
GR (1) GR3015847T3 (es)
IE (1) IE65785B1 (es)
NO (1) NO180787C (es)
PT (1) PT95928B (es)
ZA (1) ZA909212B (es)

Families Citing this family (31)

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FR2685482B1 (fr) * 1991-12-24 1994-07-29 Melet Francois Procede de numeration des hematies ou des thrombocytes et dispositif de mise en óoeuvre.
AU2095992A (en) * 1992-08-11 1994-03-24 Abbott Laboratories Flow cytometry sheath reagent for elimination of red cells with preservation of nucleated cells
DE69327775T2 (de) * 1992-11-19 2000-06-21 Sysmex Corp., Kobe Verfahren zur Vorbehandlung für Blutanalyse
EP0685994B1 (en) * 1993-02-25 2007-03-21 Abbott Laboratories Multipurpose reagent system for rapid lysis of whole blood samples
JP3320869B2 (ja) * 1993-12-22 2002-09-03 シスメックス株式会社 白血球分析用試薬
JP3467310B2 (ja) * 1994-04-21 2003-11-17 シスメックス株式会社 白血球分析用試薬及び白血球の分類方法
JP3355038B2 (ja) * 1994-08-03 2002-12-09 シスメックス株式会社 白血球分類方法
US5639630A (en) * 1995-05-16 1997-06-17 Bayer Corporation Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes
US5786224A (en) * 1995-06-29 1998-07-28 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
US5817518A (en) * 1995-12-18 1998-10-06 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US6667177B1 (en) * 1997-11-11 2003-12-23 Kowa Company, Ltd. Method for counting leukocytes and apparatus for counting leukocytes
FR2782166B1 (fr) * 1998-08-04 2000-10-06 Abx Sa Reactif pour la mesure de l'hemoglobine et la determination des leucocytes dans un echantillon de sang
FR2821428B1 (fr) * 2001-02-23 2004-08-06 Abx Sa Reactif et procede pour l'identification et le comptage de cellules biologiques
US7745219B2 (en) * 2003-07-21 2010-06-29 Siemens Healthcare Diagnostics Inc. Reagent composition for the analysis of residual white blood cells in leuko-reduced blood banking products
US7648676B2 (en) * 2004-04-20 2010-01-19 Rti Biologics, Inc. Process and apparatus for treating implants comprising soft tissue
US20060228252A1 (en) * 2004-04-20 2006-10-12 Mills C R Process and apparatus for treating implants comprising soft tissue
FR2883972B1 (fr) * 2005-03-31 2007-11-16 C2 Diagnostics Sa Procede pour l'analyse d'un echantillon de sang et appareil et reactif pour sa mise en oeuvre
CN101349644B (zh) * 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
US8102161B2 (en) * 2007-09-25 2012-01-24 Tdk Corporation Stable output in a switching power supply by smoothing the output of the secondary coil
CN101475754A (zh) * 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
EP2278331A4 (en) * 2008-05-02 2013-03-13 Arkray Inc LEUKOCYTE ANALYSIS METHOD AND ANALYSIS REAGENT FOR USE IN THE PROCESS
CN101602762B (zh) * 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
CN101726579B (zh) * 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
CN101750274B (zh) * 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
CN101988082B (zh) * 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
JP6177009B2 (ja) * 2013-06-03 2017-08-09 株式会社日立ハイテクノロジーズ 血球破壊試薬及びそれを用いる血球破壊方法
JP6738652B2 (ja) * 2016-05-26 2020-08-12 シスメックス株式会社 試料分析方法、試料分析装置および試薬
JP7143678B2 (ja) * 2018-08-24 2022-09-29 東ソー株式会社 標的細胞を検出する方法
CN109735484B (zh) * 2018-12-12 2022-07-12 中元汇吉生物技术股份有限公司 一种细胞保护剂、一种试剂及试剂的用途
CN109655607B (zh) * 2019-01-25 2021-02-23 广东菲鹏生物有限公司 红细胞裂解试剂及其应用
CN113068683B (zh) * 2021-03-03 2022-04-01 北京诚智光辉科技有限公司 一种液基细胞检查用细胞保存液及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4286963A (en) * 1979-11-23 1981-09-01 Coulter Electronics, Inc. Differential lymphoid-myeloid determination of leukocytes in whole blood
US4346018A (en) * 1980-06-16 1982-08-24 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4529705A (en) * 1983-06-06 1985-07-16 Coulter Electronics, Inc. Reagent for combined diluting and lysing whole blood
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
AU602129B2 (en) * 1985-09-06 1990-10-04 Technicon Instruments Corportion Method for the determination of a differential white blood cell count

Also Published As

Publication number Publication date
ZA909212B (en) 1991-10-30
NO180787C (no) 1997-06-18
FI93658C (fi) 1995-05-10
DE69016377T2 (de) 1995-05-24
NO904963D0 (no) 1990-11-15
GR3015847T3 (en) 1995-07-31
PT95928A (pt) 1991-09-13
FI905725A0 (fi) 1990-11-20
KR100194407B1 (ko) 1999-06-15
AU6663190A (en) 1991-05-23
JP2920690B2 (ja) 1999-07-19
FR2654744B1 (fr) 1992-03-13
ATE117803T1 (de) 1995-02-15
IE65785B1 (en) 1995-11-15
CA2030381C (en) 1997-03-25
DK0430750T3 (da) 1995-03-20
FI905725L (fi) 1991-05-21
US5232857A (en) 1993-08-03
FI93658B (fi) 1995-01-31
NO904963L (no) 1991-05-21
IE904163A1 (en) 1991-05-22
KR910010188A (ko) 1991-06-29
PT95928B (pt) 1998-01-30
NO180787B (no) 1997-03-10
EP0430750B1 (fr) 1995-01-25
JPH03266999A (ja) 1991-11-27
DE69016377D1 (de) 1995-03-09
AU634600B2 (en) 1993-02-25
EP0430750A1 (fr) 1991-06-05
FR2654744A1 (fr) 1991-05-24
CA2030381A1 (en) 1991-05-21

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