ES2081987T3 - Metodo para la determinacion de glucosa en sangre completa, y cubeta y fotometro para llevar a cabo dicho metodo. - Google Patents

Metodo para la determinacion de glucosa en sangre completa, y cubeta y fotometro para llevar a cabo dicho metodo.

Info

Publication number
ES2081987T3
ES2081987T3 ES90908120T ES90908120T ES2081987T3 ES 2081987 T3 ES2081987 T3 ES 2081987T3 ES 90908120 T ES90908120 T ES 90908120T ES 90908120 T ES90908120 T ES 90908120T ES 2081987 T3 ES2081987 T3 ES 2081987T3
Authority
ES
Spain
Prior art keywords
sample
glucose
reagent
reaction
cavity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90908120T
Other languages
English (en)
Inventor
Jan Evert Lilja
Sven-Erik Lennart Nilsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MIGRATA UK Ltd
Original Assignee
MIGRATA UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MIGRATA UK Ltd filed Critical MIGRATA UK Ltd
Application granted granted Critical
Publication of ES2081987T3 publication Critical patent/ES2081987T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/011—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells with lysing, e.g. of erythrocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0321—One time use cells, e.g. integrally moulded
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G01N2021/3181—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using LEDs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/122—Kinetic analysis; determining reaction rate
    • G01N2201/1222—Endpoint determination; reaction time determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/126—Microprocessor processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Emergency Medicine (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Measuring Cells (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

SE PONE EN CONTACTO UNA MUESTRA DE SANGRE SANA CON UN REAGENTE QUE MEDIANTE LA REACCION QUIMICA CON GLUCOSA EN LA MUESTRA DA COMO RESULTADO UN CAMBIO DETECTABLE DE COLOR EN LA CONCENTRACION (10) CUYA MAGNITUD SE DETERMINA COMO UNA MEDIDA DEL CONTENIDO DE GLUCOSA DE LA MUESTRA. LA MUESTRA SE INTRODUCE INICIALMENTE SIN DILUIR EN UNA MICROCUBETA QUE TIENE UNA CAVIDAD POR LO MENOS PARA RECIBIR LA MUESTRA. LA CAVIDAD SE TRATA PREVIAMENTE INTERNAMENTE CON EL REAGENTE DE FORMA SECA Y LA REACCION TIENE LUGAR EN LA CAVIDAD. LOS COMPONENTES ACTIVOS DEL REAGENTE CONTIENEN UN AGENTE HEMOLIZADOR POR LO MENOS PARA MOSTRAR LA GLUCOSA QUE HAY EN LAS CELULAS DE SANGRE DE LA MUESTRA PARA PERMITIR LA DETERMINACION DE GLUCOSA TOTAL Y AGENTES QUE PARTICIPAN EN LA REACCION Y QUE ASEGURAN QUE EL CAMBIO DE COLOR DE LA CONCENTRACION (10) TIENE LUGAR EN UNA GAMA DE LONGITUDES DE ONDA (14) POR LO MENOS FUERA DE LA GAMA DE ABSORCION (12) DE LA HEMOGLOBINA DE LA SANGRE. UNA MEDIDA DE LA ABSORCION SE LLEVA A CABO EN DICHA GAMA DE LONGITUDES DE ONDA DIRECTAMENTE EN LA MUESTRA QUE HAY EN LA CUBETA. SE DESCRIBEN TAMBIEN UNA CUBETA DESECHABLE PREVIAMENTE TRATADA CON TAL REAGENTE Y UN FOTOMETRO.
ES90908120T 1989-04-25 1990-04-24 Metodo para la determinacion de glucosa en sangre completa, y cubeta y fotometro para llevar a cabo dicho metodo. Expired - Lifetime ES2081987T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8901514A SE466157B (sv) 1989-04-25 1989-04-25 Saett att bestaemma glukoshalten hos helblod samt engaangskuvett foer detta

Publications (1)

Publication Number Publication Date
ES2081987T3 true ES2081987T3 (es) 1996-03-16

Family

ID=20375801

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90908120T Expired - Lifetime ES2081987T3 (es) 1989-04-25 1990-04-24 Metodo para la determinacion de glucosa en sangre completa, y cubeta y fotometro para llevar a cabo dicho metodo.

Country Status (18)

Country Link
US (1) US5866349A (es)
EP (1) EP0469097B1 (es)
JP (1) JPH0734758B2 (es)
KR (1) KR960004040B1 (es)
AT (1) ATE133453T1 (es)
AU (1) AU632569B2 (es)
BR (1) BR9007340A (es)
CA (1) CA2053284C (es)
DE (1) DE69025058T2 (es)
DK (1) DK0469097T3 (es)
ES (1) ES2081987T3 (es)
FI (1) FI102192B (es)
LT (1) LT3187B (es)
LV (1) LV10121B (es)
NO (1) NO300854B1 (es)
RU (1) RU2050545C1 (es)
SE (1) SE466157B (es)
WO (1) WO1990012890A1 (es)

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Also Published As

Publication number Publication date
JPH0734758B2 (ja) 1995-04-19
ATE133453T1 (de) 1996-02-15
AU5557690A (en) 1990-11-16
SE8901514L (sv) 1990-10-26
SE466157B (sv) 1992-01-07
EP0469097B1 (en) 1996-01-24
NO300854B1 (no) 1997-08-04
NO914182D0 (no) 1991-10-24
BR9007340A (pt) 1992-04-21
WO1990012890A1 (en) 1990-11-01
CA2053284C (en) 2001-12-11
FI102192B1 (fi) 1998-10-30
LV10121A (lv) 1994-05-10
AU632569B2 (en) 1993-01-07
DE69025058T2 (de) 1996-05-30
KR920701475A (ko) 1992-08-11
DK0469097T3 (da) 1996-02-19
US5866349A (en) 1999-02-02
NO914182L (no) 1991-10-24
JPH04504662A (ja) 1992-08-20
FI102192B (fi) 1998-10-30
LTIP276A (en) 1994-10-25
FI915021A0 (fi) 1991-10-24
LV10121B (en) 1995-02-20
DE69025058D1 (de) 1996-03-07
SE8901514D0 (sv) 1989-04-25
RU2050545C1 (ru) 1995-12-20
EP0469097A1 (en) 1992-02-05
KR960004040B1 (ko) 1996-03-25
LT3187B (en) 1995-03-27
CA2053284A1 (en) 1990-10-26

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