ES2091771T3 - Determinacion de la reactividad de los linfocitos a los antigenos especificos en la sangre. - Google Patents

Determinacion de la reactividad de los linfocitos a los antigenos especificos en la sangre.

Info

Publication number
ES2091771T3
ES2091771T3 ES90103895T ES90103895T ES2091771T3 ES 2091771 T3 ES2091771 T3 ES 2091771T3 ES 90103895 T ES90103895 T ES 90103895T ES 90103895 T ES90103895 T ES 90103895T ES 2091771 T3 ES2091771 T3 ES 2091771T3
Authority
ES
Spain
Prior art keywords
lymphocytes
blood
patient
antigen
incubated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES90103895T
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English (en)
Inventor
Robert Aaron Levine
Stephen Clark Wardlaw
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Individual
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Individual
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Publication of ES2091771T3 publication Critical patent/ES2091771T3/es
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Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Steroid Compounds (AREA)

Abstract

UNA MUESTRA DE SANGRE DE UN PACIENTE SE INCUBA CON UN ANTIGENO Y SE PRUEBA PARA RESPUESTA DE LINFOCITOS, ES DECIR, UNA ACTIVACION DE LINFOCITOS Y/O UNA CONVERSION DE LINFOCITOS A LINFOBLASTOS, LO QUE INDICA UNA EXPOSICION ANTERIOR DEL PACIENTE AL ANTIGENO. UNA RESPUESTA POSITIVA INDICA LA PRESENCIA DE EXPOSICION ANTERIOR A ENFERMEDADES PREVIAS O CONDICIONES CLINICAS COMO POR EJEMPLO ENFERMEDADES PARASITARIAS, TUBERCULOSIS, SALMONELOSIS, GONORREA, INFECCIONES CON HONGOS, INFECCIONES RIQUETSIAS, ENFERMEDAD DE LYME O ALERGENOS. LA TOTALIDAD DE LA SANGRE DEL PACIENTE ESTA INCUBADA CON EL ANTIGENO DE LA ENFERMEDAD O CONDICION PARA LA QUE SE ESTA PROBANDO AL PACIENTE. DESPUES DE UN TIEMPO APROPIADO, UN TINTE O COLORANTE FLUORESCENTE QUE TIENE UNA AFINIDAD PARA UNA CARACTERISTICA DISCRIMINANTE DE LOS LINFOCITOS O LINFOBLASTOS ACTIVADOS, COMO POR EJEMPLO: CALCIO INTRACELULAR; ANTIGENOS DE ACTIVACION DE SUPERFICIE COMO POR EJEMPLO RECEPTOR CESIONARIO; HLADR; LEU-23; Y SIMILARES. LA SANGRE INCUBADA SE INTRODUCE ENTONCES DENTRO DE UN TUBO TRANSPARENTE QUE CONTIENE UN FLOTADOR QUE CONCENTRA LAS CAPAS CONSTITUTIVAS DE LA ENVOLTURA AMORTIGUADORA DESPUES DEL CENTRIFUGADO DE LA MUESTRA DE SANGRE. LA CAPA DE LINFOCITOS CONCENTRADA SE EXAMINA ENTONCES PARA VER LA FLUORESCENCIA O COLORACION QUE ES INDICATIVA DE LA PRESENCIA DE LOS LINFOCITOS O LINFOBLASTOS ACTIVADOS Y SU CONCENTRACION. LA FLUORESCENCIA O COLORACION SE PUEDEN CALIFICAR Y/O CUANTIFICAR MEDIANTE UN INSTRUMENTO DE LECTURA.
ES90103895T 1989-04-19 1990-02-28 Determinacion de la reactividad de los linfocitos a los antigenos especificos en la sangre. Expired - Lifetime ES2091771T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34024889A 1989-04-19 1989-04-19

Publications (1)

Publication Number Publication Date
ES2091771T3 true ES2091771T3 (es) 1996-11-16

Family

ID=23332522

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90103895T Expired - Lifetime ES2091771T3 (es) 1989-04-19 1990-02-28 Determinacion de la reactividad de los linfocitos a los antigenos especificos en la sangre.

Country Status (16)

Country Link
US (2) US5360719A (es)
EP (1) EP0393334B1 (es)
JP (1) JPH0812194B2 (es)
CN (1) CN1046600A (es)
AT (1) ATE140796T1 (es)
AU (1) AU624221B2 (es)
BR (1) BR9001808A (es)
CA (1) CA2011099A1 (es)
DE (1) DE69027882T2 (es)
DK (1) DK0393334T3 (es)
ES (1) ES2091771T3 (es)
FI (1) FI901791A7 (es)
GR (1) GR3021147T3 (es)
NO (1) NO178647C (es)
RU (1) RU2061241C1 (es)
UA (1) UA24003A (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9225711D0 (en) * 1992-12-09 1993-02-03 Univ Guelph Methodology for developing a superior line of domesticated animals
GB9503406D0 (en) * 1995-02-21 1995-04-12 Haaheim Lars R Detection of antibody production
RU2143696C1 (ru) * 1995-06-27 1999-12-27 Нижегородский научно-исследовательский институт эпидемиологии и микробиологии Способ выявления сенсибилизации к лекарственным аллергенам
AU9233698A (en) * 1997-11-22 1999-06-17 Robert A. Levine Method for the detection, identification, enumeration and confirmation of circulating cancer cells and/or hematologic progenitor cells in whole blood
US5948686A (en) * 1998-03-07 1999-09-07 Robert A. Leuine Method for performing blood cell counts
GB9913819D0 (en) 1999-06-14 1999-08-11 Plasmacute As Assay
RU2156465C1 (ru) * 2000-02-01 2000-09-20 Научно-исследовательский институт медицины труда РАМН Способ диагностики микогенной сенсибилизации
US7074577B2 (en) 2002-10-03 2006-07-11 Battelle Memorial Institute Buffy coat tube and float system and method
US20130084579A1 (en) * 2002-10-03 2013-04-04 Battelle Memorial Institute Drug susceptibility using rare cell detection system
US20130084594A1 (en) * 2002-10-03 2013-04-04 Battelle Memorial Institute Drug susceptibility using rare cell detection system
GB0325483D0 (en) * 2003-10-31 2003-12-03 Plasmacute As Assay
WO2006085897A2 (en) * 2004-05-13 2006-08-17 Advanced Animal Diagnostics Microfluidic device and leucocyte antigen mediated microfluidic assay
US20090233329A1 (en) 2006-03-24 2009-09-17 Rodriguez Rodolfo R Microfluidic chamber assembly for mastitis assay
EP2274613B1 (en) * 2008-04-02 2014-06-04 Abbott Point Of Care, Inc. Method for serologic agglutination assays performed in a thin film fluid sample
JP5539314B2 (ja) * 2008-04-09 2014-07-02 アボット ポイント オブ ケア インコーポレイテッド 薄厚チャンバに含まれる薄膜状体液試料中の極めて低レベルの分析物を検出する方法
WO2011050366A2 (en) * 2009-10-23 2011-04-28 Rarecyte, Inc. Methods for changing densities on non-target particles of a suspension
CA2818173C (en) 2010-11-30 2022-05-03 Genentech, Inc. Low affinity blood brain barrier receptor antibodies and uses therefor
CN103344639B (zh) * 2013-07-11 2015-10-28 山东大学 一种快速检测尿中吡咯加合物的检测管
RU2609839C1 (ru) * 2016-03-30 2017-02-06 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России (ФГБУ "ГНЦ Институт иммунологии" ФМБА России) Способ дифференциальной диагностики гиперчувствительности к яду пчелы (apis mellifera)

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3988115A (en) * 1975-09-18 1976-10-26 Modabber Farrokh Z Diagnostic method for determining pathological condition by antigen-combining capacity of lymphocytes
US4027660A (en) * 1976-04-02 1977-06-07 Wardlaw Stephen C Material layer volume determination
US4252538A (en) * 1979-03-02 1981-02-24 Engineering & Research Associates, Inc. Apparatus and method for antibody screening, typing and compatibility testing of red blood cells
JPS5715698A (en) * 1980-06-30 1982-01-27 Nippon Electric Co Method of boring ceramic green sheet
US4436824A (en) * 1981-06-09 1984-03-13 Ortho Diagnostic Systems, Inc. Leukocyte migration through antigen containing agaroses for immunocompetence testing
US4717660A (en) * 1984-01-26 1988-01-05 Becton, Dickinson And Company Detection of bacteria by fluorescent staining in an expanded buffy coat
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US4692405A (en) * 1985-03-05 1987-09-08 Dana-Farber Cancer Institute, Inc. Monoclonal antibodies to antigen on activated human B-cells and assay therefor, protein antigenic determinant therefor and method of making same
US4689309A (en) * 1985-09-30 1987-08-25 Miles Laboratories, Inc. Test device, method of manufacturing same and method of determining a component in a sample
US4788137A (en) * 1985-10-29 1988-11-29 Dana-Farber Cancer Institute, Inc. Detection of activated T-cells
US4695553A (en) * 1985-11-01 1987-09-22 Becton Dickinson And Co., Inc. Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies
NZ214400A (en) * 1985-12-02 1989-05-29 Univ Otago Detecting infection in ruminant including conducting assay of mononuclear cell function
CA1299099C (en) * 1986-03-06 1992-04-21 Paul Richard Wood In vitro assay for detecting cell-mediated immune responses
US5006459A (en) * 1986-03-31 1991-04-09 T Cell Sciences, Inc. Therapeutic and diagnostic methods using soluble T cell surface molecules
CA1296622C (en) * 1986-08-12 1992-03-03 Jeffrey E. Anderson Method and apparatus for automated assessment of the immunoregulatory status of the mononuclear leukocyte immune system
US4835255A (en) * 1987-03-26 1989-05-30 Yale University T-cell membrane protein
EP0294216A3 (en) * 1987-06-05 1990-05-30 Interferon Sciences, Inc. A functional assay for t-lymphocytes
EP0325489B1 (en) * 1988-01-21 1994-01-19 Becton, Dickinson and Company Leu 23: Monoclonal antibody for monitoring leukocyte activation
SU1691751A1 (ru) * 1988-11-09 1991-11-15 Ленинградский педиатрический медицинский институт Способ диагностики аллергии
SU1649445A1 (ru) * 1988-12-09 1991-05-15 Научно-исследовательский институт физико-химической медицины Способ определени чувствительности организма к пыльцевым аллергенам
WO1991011719A1 (en) * 1990-01-26 1991-08-08 Washington Research Foundation Immune reactivity to expressed activated oncogenes for diagnosis and treatment of malignancy
US5215102A (en) * 1991-10-25 1993-06-01 La Mina Ltd. Capillary blood antigen testing apparatus

Also Published As

Publication number Publication date
BR9001808A (pt) 1991-06-11
UA24003A (uk) 1998-08-31
DE69027882D1 (de) 1996-08-29
EP0393334A2 (en) 1990-10-24
RU2061241C1 (ru) 1996-05-27
JPH0812194B2 (ja) 1996-02-07
ATE140796T1 (de) 1996-08-15
AU624221B2 (en) 1992-06-04
NO901700D0 (no) 1990-04-18
GR3021147T3 (en) 1996-12-31
US5480778A (en) 1996-01-02
JPH02296150A (ja) 1990-12-06
FI901791A7 (fi) 1990-10-20
US5360719A (en) 1994-11-01
EP0393334B1 (en) 1996-07-24
DK0393334T3 (da) 1996-08-26
NO178647B (no) 1996-01-22
CN1046600A (zh) 1990-10-31
FI901791A0 (fi) 1990-04-09
NO901700L (no) 1990-10-22
AU5017890A (en) 1990-10-25
EP0393334A3 (en) 1991-09-11
NO178647C (no) 1996-05-02
CA2011099A1 (en) 1990-10-19
DE69027882T2 (de) 1996-12-12

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