ES2091903T3 - Metodo para la n-miristoilacion de proteinas. - Google Patents

Metodo para la n-miristoilacion de proteinas.

Info

Publication number
ES2091903T3
ES2091903T3 ES91870031T ES91870031T ES2091903T3 ES 2091903 T3 ES2091903 T3 ES 2091903T3 ES 91870031 T ES91870031 T ES 91870031T ES 91870031 T ES91870031 T ES 91870031T ES 2091903 T3 ES2091903 T3 ES 2091903T3
Authority
ES
Spain
Prior art keywords
gene
myristoyltransferase
replication
coli
origin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91870031T
Other languages
English (en)
Inventor
Robert Joseph Duronio
Peter Olafs Olins
Jeffrey Ivan Gordon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington University in St. Louis
Original Assignee
University of Washington
Washington University in St. Louis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington University in St. Louis filed Critical University of Washington
Application granted granted Critical
Publication of ES2091903T3 publication Critical patent/ES2091903T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBE UN METODO PARA SUMINISTRAR LA CO-EXPRESION DE LA N-MYRISTOYLTRANSFERASA Y UN SUSTRATO PROTEINICO PARA DICHA N-MYRISTOYLTRANSFERASA EN E. COLI, QUE COMPRENDE LA INTRODUCCION EN LA E. COLI DE UN SISTEMA PLASMOIDAL DUAL QUE INCLUYE (A) PROMOTOR ISOPROPYL-B-D-THAIOGALACTOPYRANOSIDE-INDUCIBLE TAC, EL ENLAZADOR G10-L RIBOSOMA, UN GENE NMT, EL GENE RESISTENTE KANAMYCIN Y EL P15A ORIGEN DE LA REPLICACION EN SECUENCIA OPERABLE Y (B) EL PROMOTOR RECA, EL ENLAZADOR RIBOSOMA G10-L, UN GENE MAMMALIAN, EL GENE RESISTENTE A LA AMPILLICINA Y EL COL E1 ORIGEN DE LA REPLICACION EN SECUENCIA OPERABLE. ESTO PERMITE LA PRODUCCION DE MAMMALIAN N-MYRISTOYLPROTEINAS O PROTEINAS CONTENIENDO ANALOGOS DE ENLACE COVALENTE O RIRISTATE CON PROPIEDADES FISICO-QUIMICAS ALTERADAS.
ES91870031T 1990-02-26 1991-02-25 Metodo para la n-miristoilacion de proteinas. Expired - Lifetime ES2091903T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48510390A 1990-02-26 1990-02-26

Publications (1)

Publication Number Publication Date
ES2091903T3 true ES2091903T3 (es) 1996-11-16

Family

ID=23926912

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91870031T Expired - Lifetime ES2091903T3 (es) 1990-02-26 1991-02-25 Metodo para la n-miristoilacion de proteinas.

Country Status (9)

Country Link
US (2) US5436138A (es)
EP (1) EP0445097B1 (es)
JP (1) JPH06245775A (es)
AT (1) ATE141644T1 (es)
CA (1) CA2036965A1 (es)
DE (1) DE69121435T2 (es)
DK (1) DK0445097T3 (es)
ES (1) ES2091903T3 (es)
GR (1) GR3021484T3 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0812362A1 (en) * 1995-02-27 1997-12-17 Abbott Laboratories A plasmid for expressing modified recombinant proteins in a bacterial system
US5710044A (en) * 1995-02-27 1998-01-20 Abbott Laboratories Plasmid for expressing phosphorylated recombinant proteins in a bacterial system
US5807702A (en) * 1995-02-27 1998-09-15 Abbott Laboratories Method for expressing phosphorylated recombinant human β-casein in a bacterial system
EP0812361A1 (en) * 1995-02-27 1997-12-17 Abbott Laboratories A method for expressing modified recombinant proteins in a bacterial system
US5942254A (en) * 1995-02-27 1999-08-24 Abbott Laboratories Phosphorylated recombinant human β-casein expressed in a bacterial system
US5807700A (en) * 1995-11-27 1998-09-15 Washington University Method of screening anti-amebic agents with an E. coli mutant having a deleted adhE gene complemented by the E. histolytica EhADH2 gene
WO1998049338A1 (en) * 1997-04-30 1998-11-05 The Trustees Of The University Of Pennsylvania Bacterial interaction screening system
UA100692C2 (ru) 2007-05-02 2013-01-25 Мериал Лимитед Днк-плазмиды, имеющие повышенную экспрессию и стабильность
US9238676B2 (en) 2012-05-17 2016-01-19 Ra Pharmaceuticals, Inc. Peptide and peptidomimetic inhibitors
WO2018157150A1 (en) * 2017-02-27 2018-08-30 Duke University In vivo protein n-acylation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
US4778877A (en) * 1986-08-07 1988-10-18 Washington University Novel inhibitor peptides II
US4778878A (en) * 1986-08-07 1988-10-18 Washington University Novel inhibitor peptides I
US4709012A (en) * 1986-08-07 1987-11-24 Washington University Novel inhibitor peptides
US4740588A (en) * 1986-08-07 1988-04-26 Washington University Novel substrate peptides
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US5073571A (en) * 1988-06-16 1991-12-17 Washington University Method of inhibiting virus
US5082967A (en) * 1988-06-16 1992-01-21 Washington University Novel fatty acid analog enzyme substrates
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase

Also Published As

Publication number Publication date
EP0445097A3 (en) 1992-01-08
DE69121435D1 (de) 1996-09-26
CA2036965A1 (en) 1991-08-27
DE69121435T2 (de) 1997-02-27
US5504008A (en) 1996-04-02
JPH06245775A (ja) 1994-09-06
EP0445097B1 (en) 1996-08-21
DK0445097T3 (da) 1996-09-16
EP0445097A2 (en) 1991-09-04
GR3021484T3 (en) 1997-01-31
ATE141644T1 (de) 1996-09-15
US5436138A (en) 1995-07-25

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