ATE142272T1 - Nukleinsäure-amplifikation unter verwendung eines einzelprimers - Google Patents

Nukleinsäure-amplifikation unter verwendung eines einzelprimers

Info

Publication number
ATE142272T1
ATE142272T1 AT90300528T AT90300528T ATE142272T1 AT E142272 T1 ATE142272 T1 AT E142272T1 AT 90300528 T AT90300528 T AT 90300528T AT 90300528 T AT90300528 T AT 90300528T AT E142272 T1 ATE142272 T1 AT E142272T1
Authority
AT
Austria
Prior art keywords
polynucleotide
single stranded
sequence
analyte
stranded polynucleotide
Prior art date
Application number
AT90300528T
Other languages
English (en)
Inventor
Samuel Rose
Thomas C Goodman
Linda M Western
Martin Becker
Edwin F Ullman
Original Assignee
Behringwerke Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke Ag filed Critical Behringwerke Ag
Application granted granted Critical
Publication of ATE142272T1 publication Critical patent/ATE142272T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT90300528T 1989-01-19 1990-01-18 Nukleinsäure-amplifikation unter verwendung eines einzelprimers ATE142272T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29928289A 1989-01-19 1989-01-19
US39979589A 1989-08-29 1989-08-29

Publications (1)

Publication Number Publication Date
ATE142272T1 true ATE142272T1 (de) 1996-09-15

Family

ID=26971136

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90300528T ATE142272T1 (de) 1989-01-19 1990-01-18 Nukleinsäure-amplifikation unter verwendung eines einzelprimers

Country Status (7)

Country Link
US (2) US5827649A (de)
EP (1) EP0379369B1 (de)
JP (1) JP3109810B2 (de)
AT (1) ATE142272T1 (de)
CA (1) CA2008096A1 (de)
DE (1) DE69028325T2 (de)
ES (1) ES2093633T3 (de)

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CA2046713A1 (en) * 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
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ATE152180T1 (de) * 1992-07-31 1997-05-15 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
FR2703053B1 (fr) * 1993-03-26 1995-06-16 Genset Sa Oligonucleotides agrafes et semi-agrafes, procede de preparation et applications .
US6294323B1 (en) * 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
EP0851941B1 (de) * 1995-09-20 1999-11-17 E.I. Du Pont De Nemours And Company Eine kontrolle für die pcr
ES2279530T3 (es) * 1995-11-25 2007-08-16 Roche Diagnostics Gmbh Metodo para generar multiples acidos nucleicos bicatenarios.
AU726821B2 (en) * 1996-07-16 2000-11-23 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm
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US6326143B1 (en) 1996-11-22 2001-12-04 Roche Diagnostics Gmbh Method for generating multiple double stranded nucleic acids
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
JP4646404B2 (ja) 1998-07-02 2011-03-09 ジェン−プローブ・インコーポレーテッド 分子トーチ(torch)
EP1098996A1 (de) 1998-07-20 2001-05-16 Yale University Verfahren zum nachweis von nukleinsäuren unter verwendung zielgerichteter ligation von zweiteiligen primern
US6703228B1 (en) * 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (de) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus
EP1124990B1 (de) * 1998-10-27 2006-01-18 Affymetrix, Inc. Komplexitätsmanagement und analyse genomischer dna
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US20030152924A1 (en) * 2000-12-19 2003-08-14 Ullman Edwin F. Strand displacement detection of target nucleic acid
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
AU2005216549A1 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
JP4728663B2 (ja) * 2004-03-22 2011-07-20 シスメックス株式会社 標的物質検出用プローブセット及び標的物質検出方法
JP2005304489A (ja) * 2004-03-24 2005-11-04 Sysmex Corp 標的物質検出用プローブセット及び標的物質検出方法。
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EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US9044715B2 (en) * 2007-08-22 2015-06-02 Colorado School Of Mines Unsupported palladium alloy membranes and methods of making same
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
WO2009140374A2 (en) 2008-05-13 2009-11-19 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
WO2010068473A1 (en) * 2008-11-25 2010-06-17 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
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DK2625295T3 (da) 2010-10-08 2019-06-11 Harvard College High-throughput-immunsekvensering
EP2625320B1 (de) 2010-10-08 2019-03-27 President and Fellows of Harvard College Einzelzellen-barcoding mit hohem durchsatz
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
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US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
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Also Published As

Publication number Publication date
US6124090A (en) 2000-09-26
ES2093633T3 (es) 1997-01-01
DE69028325T2 (de) 1997-04-17
EP0379369B1 (de) 1996-09-04
JPH02268683A (ja) 1990-11-02
US5827649A (en) 1998-10-27
EP0379369A2 (de) 1990-07-25
EP0379369A3 (de) 1991-07-03
DE69028325D1 (de) 1996-10-10
JP3109810B2 (ja) 2000-11-20
CA2008096A1 (en) 1990-07-19

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