ES2094155T3 - Procedimiento para la neutralizacion de celulas sin lisis celular. - Google Patents

Procedimiento para la neutralizacion de celulas sin lisis celular.

Info

Publication number
ES2094155T3
ES2094155T3 ES90910007T ES90910007T ES2094155T3 ES 2094155 T3 ES2094155 T3 ES 2094155T3 ES 90910007 T ES90910007 T ES 90910007T ES 90910007 T ES90910007 T ES 90910007T ES 2094155 T3 ES2094155 T3 ES 2094155T3
Authority
ES
Spain
Prior art keywords
mixture
fermentation
cells
organic acid
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90910007T
Other languages
English (en)
Inventor
Virgil B Lawlis Jr
Henry G Heinsohn
Enrique F Baliu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of ES2094155T3 publication Critical patent/ES2094155T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/917—Aspergillus niger
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/94—Saccharomyces
    • Y10S435/942—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/823—Lower fungi, e.g. mold
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/823—Lower fungi, e.g. mold
    • Y10S530/824—Yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • External Artificial Organs (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fertilizers (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

ESTA INVENCION PROPORCIONA UN METODO PARA MATAR LAS CELULAS EN EL PROCESO DE FERMENTACION A FIN DE PREPARAR LA MEZCLA DE FERMENTACION PARA SU PROCESAMIENTO PARA RECUPERAR O EXTRAER UN PRODUCTO DESEADO DE LA MEZCLA FERMENTADA. UN METODO PREFERENTE DE ESTA INVENCION COMPRENDE EL AJUSTAR EL PH DE LA MEZCLA DE FERMENTACION ALREDEDOR DE 4.75 UTILIZANDO UN ACIDO MINERAL, DESPUES AÑADIR SUFICIENTE CANTIDAD DE ACIDO ACETICO A LA MEZCLA PARA AFECTAR LA MUERTE DE LAS CELULAS SUSTANCIALMENTE COMPLETA EN LA MEZCLA. PODRA UTILIZARSE UNA DE LAS SALES DEL ACIDO ACETICO. PODRA AÑADIRSE EL ACIDO ACETICO O UNA SAL, DESPUES SE AJUSTARA EL PH AL NIVEL DESEADO. PODRAN UTILIZARSE OTROS ACIDOS ORGANICOS, EN CUYO CASO EL PH DE LA MEZCLA SE AJUSTA AL PKA DEL ACIDO ORGANICO SELECCIONADO ANTES DE AÑADIRLO A LA MEZCLA. EL METODO DE ESTA INVENCION ES UTIL PARA DETENER EL CRECIMIENTO Y MUERTE DE LAS CELULAS DE CUALQUIER MICROORGANISMO UTILIZADO EN CULTIVOS O EN PROCEDIMIENTOS DE FERMENTACION TALES COMO LAS LEVADURAS QUELOS CONTIENEN, LAS BACTERIAS Y LOS HONGOS. ESTE METODO ES PARTICULARMENTE UTIL EN LOS SISTEMAS DONDE SE DESEA MATAR LAS CELULAS SIN LISIS.
ES90910007T 1989-06-13 1990-06-13 Procedimiento para la neutralizacion de celulas sin lisis celular. Expired - Lifetime ES2094155T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36594589A 1989-06-13 1989-06-13

Publications (1)

Publication Number Publication Date
ES2094155T3 true ES2094155T3 (es) 1997-01-16

Family

ID=23441050

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90910007T Expired - Lifetime ES2094155T3 (es) 1989-06-13 1990-06-13 Procedimiento para la neutralizacion de celulas sin lisis celular.

Country Status (13)

Country Link
US (1) US5378621A (es)
EP (1) EP0477280B1 (es)
JP (1) JP2683760B2 (es)
AT (1) ATE142688T1 (es)
AU (1) AU641169B2 (es)
CA (1) CA2058633C (es)
DE (1) DE69028529T2 (es)
DK (1) DK0477280T3 (es)
ES (1) ES2094155T3 (es)
FI (1) FI100537B (es)
NO (2) NO307186B1 (es)
NZ (1) NZ234059A (es)
WO (1) WO1990015861A1 (es)

Families Citing this family (193)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL95019A0 (en) * 1989-08-09 1991-06-10 Mycogen Corp Process for encapsulation of biologicals
AU658578B2 (en) * 1991-06-26 1995-04-27 Bio-Technology General Corporation Purification of recombinant apolipoprotein E from bacteria
DE19851156C1 (de) * 1998-11-06 2000-04-27 Merck Patent Gmbh Verfahren zur Isolierung von Plasmid-DNA
US7579176B2 (en) 2003-10-15 2009-08-25 Newlight Technologies, Llc Method for the production of polyhydroxyalkanoic acid
IN2009MU00380A (es) * 2009-02-18 2010-04-02
WO2010096673A1 (en) * 2009-02-20 2010-08-26 Danisco Us Inc. Fermentation broth formulations
US20120165500A1 (en) 2009-08-27 2012-06-28 Newlight Technologies, Llc Process for the production of polyhydroxyalkanoates
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
US9187742B2 (en) 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20130212746A1 (en) 2010-08-30 2013-08-15 Novoyzmes A/S Polypeptides Having Hemicellulolytic Activity And Polynucleotides Encoding Same
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
DK2735611T3 (en) 2010-08-30 2019-01-28 Novozymes As POLYPEPTIDES WITH CELLULOLYSE INCREASING ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2012030845A2 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
US9816082B2 (en) 2010-09-30 2017-11-14 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US10246691B2 (en) 2010-09-30 2019-04-02 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
ES2563209T3 (es) 2010-10-01 2016-03-11 Novozymes, Inc. Variantes de beta-glucosidasa y polinucleótidos que codifican las mismas
WO2012061517A1 (en) 2010-11-02 2012-05-10 Novozymes, Inc. Methods of pretreating cellulosic material with a gh61 polypeptide
EP2635594B1 (en) 2010-11-04 2017-01-11 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112013009026A2 (pt) 2010-11-12 2016-07-12 Novozymes Inc polipeptídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e método para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, polinucleotídeo isolado, e, formulação de caldo integral ou composição de cultura de célula
WO2012068509A1 (en) 2010-11-18 2012-05-24 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
EP2668269B1 (en) 2011-01-26 2018-01-03 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
CN103384678B (zh) 2011-02-23 2017-01-18 诺维信股份有限公司 具有纤维素水解增强活性的多肽及其编码多核苷酸
CN103608461B (zh) 2011-03-09 2016-08-03 诺维信公司 增加多肽的纤维素分解增强活性的方法
WO2012122477A1 (en) 2011-03-10 2012-09-13 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US9879294B2 (en) 2011-03-25 2018-01-30 Novozymes A/S Methods for degrading or converting cellulosic material
US20140080182A1 (en) 2011-03-31 2014-03-20 Novozymes, Inc. Cellulose Binding Domain Variants and Polynucleotides Encoding Same
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
EP2702153B1 (en) 2011-04-28 2018-12-12 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
DK2702162T3 (da) 2011-04-29 2020-05-18 Novozymes Inc Fremgangsmåder til forbedring af nedbrydningen eller omdannelsen af celluloseholdigt materiale
WO2012159007A1 (en) 2011-05-19 2012-11-22 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
US8993286B2 (en) 2011-05-19 2015-03-31 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
CN103890165A (zh) 2011-08-24 2014-06-25 诺维信股份有限公司 纤维素分解酶组合物及其用途
US9663772B2 (en) 2011-08-24 2017-05-30 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
US20140308705A1 (en) 2011-09-20 2014-10-16 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
CA2850070A1 (en) 2011-09-30 2013-04-04 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
DK2760885T3 (en) 2011-09-30 2017-10-30 Novozymes Inc CHEMICAL POLYPEPTIDES WITH BETA-GLUCOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2013064075A1 (en) 2011-10-31 2013-05-10 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2780449B1 (en) 2011-11-18 2018-04-11 Novozymes, Inc. Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
US10351834B2 (en) 2011-11-21 2019-07-16 Novozymes, Inc. GH61 polypeptide variants and polynucleotides encoding same
WO2013075644A1 (en) 2011-11-22 2013-05-30 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
US9624481B2 (en) 2011-12-01 2017-04-18 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
US12060597B2 (en) 2011-12-02 2024-08-13 Newlight Technologies, Inc. Polyhydroxyalkanoate production methods and systems for same
EP3272862A1 (en) 2011-12-16 2018-01-24 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
BR112014014697A2 (pt) 2011-12-19 2020-10-27 Novozymes, Inc. polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos para produzir um polipeptídeo e uma proteína, para gerar oxigênio molecular, e para remover peróxido de hidrogênio do tecido, processos para degradar ou converter um material celulósico, e para produzir um produto de fermentação, e, formulação de caldo integral ou composição de cultura de células
CA2878019A1 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
EP2794899A1 (en) 2011-12-21 2014-10-29 Novozymes, Inc. Methods for determining the degradation of a biomass material
US20200347417A1 (en) 2012-03-29 2020-11-05 Newlight Technologies, Inc Polyhydroxyalkanoate production methods and materials and microorganisms used in same
US9085784B1 (en) 2012-03-29 2015-07-21 Newlight Technologies, Llc Polyhydroxyalkanoate production methods and materials and microorganisms used in same
US10227579B2 (en) 2012-04-27 2019-03-12 Novozymes A/S GH61 polypeptide variants and polynucleotides encoding same
AR090971A1 (es) 2012-05-07 2014-12-17 Novozymes As Polipeptidos que tienen actividad de degradacion de xantano y polinucleotidos que los codifican
EP2870256A1 (en) * 2012-07-06 2015-05-13 Novozymes A/S Inactivation of a production strain using a fatty acid
MX2015001969A (es) 2012-08-17 2015-05-15 Novozymes As Variantes de asparaginasa termoestables y polinucleotidos que las codifican.
WO2014092832A2 (en) 2012-09-19 2014-06-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP3586610B1 (en) 2012-10-08 2024-09-25 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2014066141A2 (en) 2012-10-24 2014-05-01 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112015013421A2 (pt) 2012-12-14 2017-11-14 Novozymes As E Novozymes Inc célula hospedeira microbiana transgênica, polipeptídeo isolado, métodos de produção de um polipeptídeo e de uma proteína, processos de degradação de um material celulósico, de produção de um produto de fermentação e de fermentação de um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
US20150337280A1 (en) 2012-12-19 2015-11-26 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
BR112015014624A2 (pt) 2012-12-24 2017-10-10 Novozymes As polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, método para tratamento de têxtil
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
TR201819061T4 (tr) 2013-04-18 2019-01-21 Novozymes As Proteaz Aktivitesine Sahip Polipeptitler Ve Bunları Kodlayan Polinükleotitler
DK2994529T3 (en) 2013-05-10 2019-03-04 Novozymes As Polypeptides with xylanase activity and polynucleotides encoding them
CN105209613A (zh) 2013-05-17 2015-12-30 诺维信公司 具有α淀粉酶活性的多肽
JP2016521579A (ja) 2013-06-21 2016-07-25 ノボザイムス アクティーゼルスカブ バチルス属（Ｂａｃｉｌｌｕｓ）における分泌シグナルを含まないポリペプチドの生成
EP3022300B1 (en) 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
WO2015022429A1 (en) 2013-08-15 2015-02-19 Novozymes A/S Polypeptides having beta-1,3-galactanase activity and polynucleotides encoding same
US20160298154A1 (en) 2013-09-04 2016-10-13 Novozymes A/S Processes for Increasing Enzymatic Hydrolysis of Cellulosic Material
WO2015081139A1 (en) 2013-11-26 2015-06-04 Novozymes A/S Enzyme compositions and uses thereof
EP3097192B1 (en) 2014-01-22 2018-07-25 Novozymes A/S Pullulanase variants and polynucleotides encoding same
US20170015950A1 (en) 2014-04-01 2017-01-19 Novozymes A/S Polypeptides having alpha amylase activity
MX376770B (es) 2014-04-11 2025-03-07 Novozymes As Composición detergente.
CA2950273C (en) 2014-05-30 2022-06-21 Novozymes A/S Variants of gh family 11 xylanase and polynucleotides encoding same
AU2015269381B2 (en) 2014-06-06 2019-04-18 Novozymes A/S Enzyme compositions and uses thereof
WO2015189371A1 (en) 2014-06-12 2015-12-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3183339A1 (en) 2014-08-20 2017-06-28 Novozymes A/S Xyloglucan endotransglycosylase variants and polynucleotides encoding same
BR112017003847B1 (pt) 2014-08-28 2024-01-09 Renescience A/S Processo para solubilização de resíduos tais como resíduos sólidos urbanos (rsu), e processo para produção de um produto de fermentação
EP3594335B1 (en) 2014-09-05 2024-05-01 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
BR112017009320A2 (pt) 2014-11-04 2017-12-19 Novozymes As ração animal ou aditivo de ração animal, uso de ração animal ou aditivo de ração animal, e, métodos para preparação de uma ração animal, para melhoria do valor nutricional de uma ração animal, para tratamento de proteínas aumento da digestibilidade e/ou solubilidade da proteína, para melhoria de um ou mais parâmetros de desempenho em um animal e para produção de um polipeptídeo.
WO2016079305A1 (en) 2014-11-20 2016-05-26 Novozymes A/S Alicyclobacillus variants and polynucleotides encoding same
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
WO2016090059A1 (en) 2014-12-02 2016-06-09 Novozymes A/S Laccase variants and polynucleotides encoding same
MX383142B (es) 2014-12-19 2025-03-13 Novozymes As Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa.
US10557127B2 (en) 2015-02-24 2020-02-11 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2016145363A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Multi-stage enzymatic hydrolysis of lignocellulosic biomass employing an oxidoreductase with an aa9 polypeptide
WO2016145358A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Enzymatic hydrolysis with hemicellulolytic enzymes
DK3268484T3 (da) 2015-03-12 2020-08-17 Beta Renewables Spa Enzymatisk flertrinshydrolyse af lignocelluloseholdig biomasse
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
WO2016180749A1 (en) 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
AU2016259703B2 (en) 2015-05-08 2021-12-23 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
BR112017021489A2 (pt) 2015-05-27 2018-07-03 Novozymes A/S polipeptídeo tendo atividade de celobio-hidrolase, composição, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos de produção do polipeptídeo.
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CA2981342A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
WO2016207373A1 (en) 2015-06-26 2016-12-29 Novozymes A/S Polypeptides having peroxygenase activity
BR112017028063B1 (pt) 2015-06-26 2022-05-17 Novozymes A/S Método para produção de um extrato de café
US10392742B2 (en) 2015-06-26 2019-08-27 Novozymes A/S Biofinishing system
US10577594B2 (en) 2015-07-24 2020-03-03 Novozymes, Inc. Polypeptides having arabinofuranosidase activity and polynucleotides encoding same
US20180216089A1 (en) 2015-07-24 2018-08-02 Novozymes, Inc. Polypeptides Having Beta-Xylosidase Activity And Polynucleotides Encoding Same
EP3344761A1 (en) 2015-09-04 2018-07-11 Novozymes A/S Methods of inhibiting aa9 lytic polysaccharide monooxygenase catalyzed inactivation of enzyme compositions
CA2991114A1 (en) 2015-09-17 2017-03-23 Novozymes A/S Polypeptides having xanthan degrading activity and polynucleotides encoding same
US10479984B2 (en) 2015-09-22 2019-11-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP3359657B1 (en) 2015-10-07 2020-04-01 Novozymes A/S Polypeptides
WO2017070219A1 (en) 2015-10-20 2017-04-27 Novozymes A/S Lytic polysaccharide monooxygenase (lpmo) variants and polynucleotides encoding same
WO2017076421A1 (en) 2015-11-02 2017-05-11 Renescience A/S Solubilization of msw with blend enzymes
MX2018008051A (es) 2016-01-29 2018-08-23 Novozymes As Variantes de beta-glucanasa y polinucleotidos que las codifican.
BR112018067371A2 (pt) 2016-03-02 2019-01-15 Novozymes As variante de celobio-hidrolase, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, célula hospedeira recombinante, planta, parte de planta ou célula de planta transgênica, métodos para produção e para obtenção de uma variante de celobio-hidrolase, planta trangênica, e, processos para degradação de um material celulósico, para produção de um produto de fermentação e para fermentação de um material celulósico
US11965189B2 (en) 2016-03-24 2024-04-23 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2017202979A1 (en) 2016-05-24 2017-11-30 Novozymes A/S Polypeptides having alpha-galactosidase activity and polynucleotides encoding same
BR112018073890A2 (pt) 2016-05-24 2019-02-26 Novozymes As composição, grânulo, aditivo de ração para animal, ração para animal, formulação líquida, uso da composição, do grânulo, do aditivo de ração para animal ou da formulação líquida, polipeptídeo isolado, métodos para liberar galactose de material à base de planta, para melhorar um ou mais parâmetros de desempenho de um animal e para produzir o polipeptídeo, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, e, célula hospedeira recombinante.
US10808268B2 (en) 2016-05-24 2020-10-20 Novozymes A/S Polypeptides having alpha-galactosidase activity and polynucleotides encoding same
CN109196098A (zh) 2016-05-24 2019-01-11 诺维信公司 包含具有半乳聚糖酶活性的多肽和具有β-半乳糖苷酶活性的多肽的组合物
WO2017205535A1 (en) 2016-05-27 2017-11-30 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2018002261A1 (en) 2016-07-01 2018-01-04 Novozymes A/S Detergent compositions
EP3481962A1 (en) 2016-07-07 2019-05-15 Novozymes A/S Methods of producing a fermentation product in trichoderma
CA3029113A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Xylanase variants and polynucleotides encoding same
WO2018007573A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Detergent compositions with galactanase
WO2018007153A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP3485008B1 (en) 2016-07-18 2024-01-31 Novozymes A/S Lipase variants, polynucleotides encoding same and the use thereof
CN109477083B (zh) 2016-07-20 2023-06-06 诺维信公司 热稳定性宏基因组碳酸酐酶及其用途
WO2018026868A1 (en) 2016-08-01 2018-02-08 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US20210284933A1 (en) 2016-10-25 2021-09-16 Novozymes A/S Detergent compositions
WO2018085370A1 (en) 2016-11-02 2018-05-11 Novozymes A/S Processes for reducing production of primeverose during enzymatic saccharification of lignocellulosic material
CN110072997A (zh) 2016-11-23 2019-07-30 诺维信公司 具有蛋白酶活性的多肽和编码其的多核苷酸
WO2018134386A1 (en) 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
BR112019017271A2 (pt) 2017-02-20 2020-04-14 Novozymes As enzima lipolítica para uso em panificação
US11208639B2 (en) 2017-03-31 2021-12-28 Novozymes A/S Polypeptides having DNase activity
WO2018177936A1 (en) 2017-03-31 2018-10-04 Novozymes A/S Polypeptides having dnase activity
US11149233B2 (en) 2017-03-31 2021-10-19 Novozymes A/S Polypeptides having RNase activity
CN110651040A (zh) 2017-03-31 2020-01-03 诺维信公司 具有dna酶活性的多肽
EP3607039A1 (en) 2017-04-04 2020-02-12 Novozymes A/S Polypeptides
CN114480034A (zh) 2017-04-04 2022-05-13 诺维信公司 糖基水解酶
US11499144B2 (en) 2017-06-22 2022-11-15 Novozymes A/S Xylanase variants and polynucleotides encoding same
US11584783B2 (en) 2017-06-28 2023-02-21 Novozymes A/S Processes for producing a fermentation product
US11220679B2 (en) 2017-08-08 2022-01-11 Novozymes A/S Polypeptides having trehalase activity
EP4603591A3 (en) 2017-08-31 2025-10-22 Novozymes A/S Polypeptides having d-psicose 3-epimerase activity and polynucleotides encoding same
AU2018322865B2 (en) 2017-09-01 2023-11-09 Novozymes A/S Animal feed additives comprising polypeptide having protease activity and uses thereof
EP4501130A3 (en) 2017-09-01 2025-04-23 Novozymes A/S Animal feed additives comprising a polypeptide having protease activity and uses thereof
US11746310B2 (en) 2017-10-02 2023-09-05 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
CN111373036A (zh) 2017-10-02 2020-07-03 诺维信公司 具有甘露聚糖酶活性的多肽和编码它们的多核苷酸
CN111148830A (zh) 2017-10-04 2020-05-12 诺维信公司 具有蛋白酶活性的多肽和编码其的多核苷酸
CN111417726A (zh) 2017-10-23 2020-07-14 诺维信公司 通过与前肽共表达改善蛋白酶的表达
DE102017125559A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine ii enthalten
WO2019096903A1 (en) 2017-11-20 2019-05-23 Novozymes A/S New galactanases (ec 3.2.1.89) for use in soy processing
BR112020011173A2 (pt) 2017-12-08 2020-11-17 Novozymes A/S variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido.
DK3720955T5 (da) 2017-12-08 2024-08-26 Novozymes As Alfa-amylasevarianter og polynukleotider, der koder for dem
WO2019197318A1 (en) 2018-04-09 2019-10-17 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2020070199A1 (en) 2018-10-03 2020-04-09 Novozymes A/S Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same
BR112021011384A2 (pt) 2018-12-12 2022-05-17 Novozymes As Polipeptídeo isolado, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo isolado, célula hospedeira recombinante, método de produção de um polipeptídeo, planta, parte da planta ou célula da planta transgênica, e, processos para degradação de um material celulósico ou hemicelulósico, para produção de um produto de fermentação e de fermentação de um material celulósico ou hemicelulósico
CN113366103A (zh) 2018-12-21 2021-09-07 诺维信公司 具有肽聚糖降解活性的多肽以及编码其的多核苷酸
US12492391B2 (en) 2019-01-30 2025-12-09 Novozymes A/S Cognate foldase co-expression
BR112021014873A2 (pt) 2019-01-31 2021-10-05 Novozymes A/S Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação
EP3935160A1 (en) 2019-03-08 2022-01-12 Novozymes A/S Means and methods for improving protease expression
EP3942033A1 (en) 2019-03-18 2022-01-26 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
JP7725365B2 (ja) 2019-03-21 2025-08-19 ノボザイムス アクティーゼルスカブ α－アミラーゼ変異体及びこれをコードするポリヌクレオチド
WO2020201403A1 (en) 2019-04-03 2020-10-08 Novozymes A/S Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions
EP4031661A1 (en) 2019-09-16 2022-07-27 Novozymes A/S Polypeptides having beta-glucanase activity and polynucleotides encoding same
WO2021064068A1 (en) 2019-10-03 2021-04-08 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
JP2023506305A (ja) 2019-12-19 2023-02-15 ノボザイムス アクティーゼルスカブ キシラナーゼバリアント及びそれをコードするポリヌクレオチド
US20220411773A1 (en) 2019-12-20 2022-12-29 Novozymes A/S Polypeptides having proteolytic activity and use thereof
WO2021152120A1 (en) 2020-01-31 2021-08-05 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP4097227A1 (en) 2020-01-31 2022-12-07 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP4103709A2 (en) 2020-02-10 2022-12-21 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
US20230235367A1 (en) 2020-02-10 2023-07-27 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20230167384A1 (en) 2020-04-21 2023-06-01 Novozymes A/S Cleaning compositions comprising polypeptides having fructan degrading activity
US20230313209A1 (en) 2020-08-18 2023-10-05 Novozymes A/S Dispersins expressed with amylase signal peptides
EP4204551B1 (en) 2020-08-25 2025-09-17 Novozymes A/S Variants of a family 44 xyloglucanase
WO2022043563A1 (en) 2020-08-28 2022-03-03 Novozymes A/S Polyester degrading protease variants
US20250346879A1 (en) 2020-10-07 2025-11-13 Novozymes A/S Alpha-amylase variants
CN116438309A (zh) 2020-11-02 2023-07-14 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
EP4291646A2 (en) 2021-02-12 2023-12-20 Novozymes A/S Alpha-amylase variants
EP4359518A1 (en) 2021-06-23 2024-05-01 Novozymes A/S Alpha-amylase polypeptides
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023118565A1 (en) 2021-12-23 2023-06-29 Novozymes A/S Reduction of residual dna in microbial fermentation products
US20250215412A1 (en) 2022-03-08 2025-07-03 Novozymes A/S Fusion Polypeptides with Deamidase Inhibitor and Deamidase Domains
EP4242303A1 (en) 2022-03-08 2023-09-13 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
JP2025525309A (ja) 2022-07-15 2025-08-05 ノボザイムス アクティーゼルスカブ デアミダーゼ阻害剤活性を有するポリペプチド
WO2024056643A1 (en) 2022-09-15 2024-03-21 Novozymes A/S Fungal signal peptides
JP2026508744A (ja) 2022-12-05 2026-03-12 ノボザイムス アクティーゼルスカブ プロテアーゼバリアント、及びそれをコードするポリヌクレオチド
CN120265779A (zh) 2022-12-05 2025-07-04 诺维信公司 经修饰的rna聚合酶活性
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
EP4638724A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
EP4638684A1 (en) 2022-12-21 2025-10-29 Novozymes A/S Recombinant protease for cell detachment
EP4389865A1 (en) 2022-12-21 2024-06-26 Novozymes A/S Recombinant protease for cell detachment
CN120476196A (zh) 2022-12-21 2025-08-12 诺维信公司 用于细胞分离的微生物蛋白酶
CN121464215A (zh) 2023-07-10 2026-02-03 诺维信公司 人工信号肽
WO2025133162A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Recombinant protein expression
WO2025191164A2 (en) 2024-03-12 2025-09-18 Novozymes A/S Use of chymopapain in cell detachment
WO2025226596A1 (en) 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides
WO2025233389A1 (en) 2024-05-09 2025-11-13 Novozymes A/S Stabilized liquid deamidase compositions
WO2025257254A1 (en) 2024-06-12 2025-12-18 Novozymes A/S Lipases and lipase variants and the use thereof
WO2026017381A1 (en) 2024-07-17 2026-01-22 Novozymes A/S Optimized bacillus host cells
WO2026068782A1 (en) 2024-09-30 2026-04-02 Novozymes A/S Protease variants and polynucleotides encoding same
WO2026073574A1 (en) 2024-10-01 2026-04-09 Novozymes A/S Codon optimized deamidase expression

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1908225A (en) * 1931-02-05 1933-05-09 Pfizer Charles & Co Process of fermentation
US3134723A (en) * 1961-12-15 1964-05-26 Grain Processing Corp Method of refining fungal enzyme preparations
US3345268A (en) * 1964-08-03 1967-10-03 Grain Processing Corp Process of purifying a transglucosidase and glucoamylase-containing fungal enzyme preparation
JPS5612435B1 (es) * 1971-03-29 1981-03-20
US3779868A (en) * 1971-07-15 1973-12-18 P Nikolaev Microbiological method of producing edible acetic acid
US3890198A (en) * 1971-12-14 1975-06-17 Kumiai Chemical Industry Co Cell wall-lysing complex enzymes and a process for the production thereof
JPS5413496B2 (es) * 1972-10-17 1979-05-31
US3917510A (en) * 1973-04-11 1975-11-04 Kirin Brewery Lysis of yeast cell walls
FR2286191A1 (fr) * 1974-09-30 1976-04-23 Pasteur Institut Principes nutritifs a base de proteines, compositions alimentaires contenant de tels principes nutritifs et leur procede d'obtention
US4647458A (en) * 1981-09-25 1987-03-03 Kabushiki Kaisha Ueno Seiyaku Oyo Kenkyujo Liquid bactericide for foods and food processing machines or utensils, employing a synergistic mixture of ethyl alcohol, an organic acid and phosphoric acid
US4596778A (en) * 1983-07-06 1986-06-24 Phillips Petroleum Company Single cell protein from sulfur energy sources
US4601986A (en) * 1983-07-29 1986-07-22 Phillips Petroleum Company Protein product of reduced nucleic acid content and low allergenicity
CA1231053A (en) * 1984-01-09 1988-01-05 Capetrol International, Inc. Germicides having ph values of less than about l
JPS60180581A (ja) * 1984-02-28 1985-09-14 Nakano Vinegar Co Ltd 新菌種アセトバクタ−・アルトアセチゲネス
US4725544A (en) * 1986-04-25 1988-02-16 Tan Larry U Method for purifying xylanase
DE3618076A1 (de) * 1986-06-02 1987-12-03 Kernforschungsanlage Juelich Verfahren zur mikrobiellen anaeroben gewinnung von essigsaeure
DE3908421A1 (de) * 1989-03-15 1990-09-20 Forschungszentrum Juelich Gmbh Verfahren zur abreicherung von salz aus biomassesuspensionen

Also Published As

Publication number Publication date
JP2683760B2 (ja) 1997-12-03
DK0477280T3 (es) 1997-02-10
DE69028529T2 (de) 1997-02-27
EP0477280A1 (en) 1992-04-01
FI100537B (fi) 1997-12-31
EP0477280A4 (en) 1992-05-06
NZ234059A (en) 1992-05-26
CA2058633C (en) 2000-03-21
JPH05500604A (ja) 1993-02-12
WO1990015861A1 (en) 1990-12-27
DE69028529D1 (de) 1996-10-17
NO976103D0 (no) 1997-12-29
NO914888D0 (no) 1991-12-12
US5378621A (en) 1995-01-03
FI915810A0 (fi) 1991-12-10
NO914888L (no) 1992-02-12
AU5855190A (en) 1991-01-08
ATE142688T1 (de) 1996-09-15
CA2058633A1 (en) 1990-12-14
NO976103L (no) 1997-12-29
AU641169B2 (en) 1993-09-16
NO307186B1 (no) 2000-02-21
EP0477280B1 (en) 1996-09-11

Similar Documents

Publication Publication Date Title
ES2094155T3 (es) Procedimiento para la neutralizacion de celulas sin lisis celular.
ATE252646T1 (de) Produktion von beta -glukan-mannan präparationen mittels autolyse von zellen unter bestimmten ph-, temperatur- und zeit bedingungen
Selwal et al. Tannase production by Penicillium atramentosum KM under SSF and its applications in wine clarification and tea cream solubilization
KURBANOĞLU Production of single-cell protein from ram horn hydrolysate
RU2019527C1 (ru) Способ очистки почв от нефтяных загрязнений
GB1384179A (en) Treatment of microorganism cells
KR870004130A (ko) 재조합균세포의 배양방법
Bhushan et al. Lipase production from an alkalophilic yeast sp. by solid state fermentation
Riaz et al. Characterization of a bacterial keratinolytic protease for effective degradation of chicken feather waste into feather protein hydrolysates
GB1466078A (en) Treatment of proteinaceous material
US4133904A (en) Treatment of single cell protein
US5801034A (en) Method for killing cells without lysis and enzyme recovery
Krogstad et al. Effects of extracts of crude and composted bark from spruce on some selected biological systems
ES2089034T3 (es) Material de paredes celulares fungicas con propiedades floculantes y metodo para su produccion.
KR19990083143A (ko) 비타민 비6의 효소적 생산
Lawlis et al. Killing cells without lysis in a method for enzyme recovery from a fermentation broth
Hatanaka et al. Isolation and characterization of carboxylesterase from vinyl acetate-assimilating bacterium isolated from soil
Delavier-Klutchko et al. Assimilation of ammonia during sporogenesis of Saccharomyces cerevisiae: effect of ammonia and glutamine
Takahashi et al. Lipase formation by micro-organisms grown on hydrocarbons
US2744050A (en) Processes for increasing the yield of enzymes
Gillespie Properties of Wool Root Phosphatases
Nesterova et al. Properties of proteolytic enzymes isolated from a thermophilic strain of Micromonospora vulgaris 42
DE102004041717A1 (de) Restriktions-Modifikationssystem von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren
JPH0141316B2 (es)
GB1352860A (en) Process for racemizing alpha-amino-epsilon-caprolactam and the hydrolysis thereof to l-lysine

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 477280

Country of ref document: ES