ES2094928T3 - Metodo de hibridacion in situ. - Google Patents

Metodo de hibridacion in situ.

Info

Publication number
ES2094928T3
ES2094928T3 ES92918705T ES92918705T ES2094928T3 ES 2094928 T3 ES2094928 T3 ES 2094928T3 ES 92918705 T ES92918705 T ES 92918705T ES 92918705 T ES92918705 T ES 92918705T ES 2094928 T3 ES2094928 T3 ES 2094928T3
Authority
ES
Spain
Prior art keywords
cell
situ hybridization
hybridization method
subcellular
marked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92918705T
Other languages
English (en)
Inventor
David A Zarling
Cornelia J Calhoun
Elissa P Sena
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daikin Industries Ltd
Original Assignee
Daikin Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daikin Industries Ltd filed Critical Daikin Industries Ltd
Application granted granted Critical
Publication of ES2094928T3 publication Critical patent/ES2094928T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

SE DESCRIBE UN METODO PARA IDENTIFICAR LA PRESENCIA DE UNA SECUENCIA DIANA CONOCIDA DE UN ACIDO NUCLEICO CONTENIDO EN UNA ESTRUCTURA BIOLOGICA CELULAR O SUBCELULAR FIJA. SUMINISTRANDO UN COMPLEJO SONDA ESTABLE DE CADENA SENCILLA/RECA DE PRESENTACIONMARCADA, A LA ESTRUCTURA CELULAR O SUBCELULAR, LA SECUENCIA OBJETIVO PUEDE SER MARCADA EFECTIVAMENTE MEDIANTE HIBRIDIZACION IN SITU, PERMITIENDO QUE LA SECUENCIA DIANA SEA VISUALIZADA HISTOLOGICA Y MICROSCOPICAMENTE O DETECTADA MEDIANTE CITOMETRIA IN SITU O TECNICAS DE CLASIFICACION DE FLUJO CELULAR.
ES92918705T 1991-09-04 1992-09-03 Metodo de hibridacion in situ. Expired - Lifetime ES2094928T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/755,291 US5506098A (en) 1991-09-04 1991-09-04 In situ hybridization method

Publications (1)

Publication Number Publication Date
ES2094928T3 true ES2094928T3 (es) 1997-02-01

Family

ID=25038542

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92918705T Expired - Lifetime ES2094928T3 (es) 1991-09-04 1992-09-03 Metodo de hibridacion in situ.

Country Status (13)

Country Link
US (2) US5506098A (es)
EP (1) EP0614492B1 (es)
JP (1) JP2819830B2 (es)
KR (1) KR100245283B1 (es)
AT (1) ATE145674T1 (es)
AU (1) AU664045B2 (es)
CA (1) CA2116214C (es)
DE (1) DE69215533T2 (es)
DK (1) DK0614492T3 (es)
ES (1) ES2094928T3 (es)
FI (1) FI941015L (es)
NO (1) NO940746L (es)
WO (1) WO1993005177A1 (es)

Families Citing this family (49)

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JP2745816B2 (ja) * 1990-05-07 1998-04-28 ダイキン工業株式会社 ダブルｄループ形成の診断応用
US20020108136A1 (en) * 1997-03-21 2002-08-08 Sri Transgenic animals produced by homologous sequence targeting
DE4216949C2 (de) * 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
CA2167804A1 (en) * 1993-07-20 1995-02-02 Robert H. Singer In vivo nucleic acid hybridization method
CA2156743C (en) * 1993-12-28 2001-02-27 Koji Kigawa In-situ hybridization method using reca protein and reca protein having marker or ligand for use in said method
US5766891A (en) * 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US6022689A (en) * 1995-04-07 2000-02-08 University Of New Mexico Situ hybridization slide processes
US5750340A (en) * 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
DE69730145T2 (de) * 1996-08-29 2005-07-14 Tapestry Pharmaceuticals, Inc., Boulder VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE
FR2755148B1 (fr) * 1996-10-31 1999-01-29 Calvo Fabien Procede destine a mettre en evidence l'etat d'une cellule maligne et procede de traitement
JP3891620B2 (ja) * 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
DE19806962B4 (de) * 1997-02-22 2004-08-05 Universität Heidelberg Markierung von Nukleinsäuren mit speziellen Probengemischen
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6194146B1 (en) 1997-06-13 2001-02-27 Bioseparations, Inc. Situ and in vitro hybridization method and buffer
US5948617A (en) * 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
EP1002126A4 (en) * 1997-06-25 2004-11-03 Life Technologies Inc IMPROVED METHOD FOR ISOLATING AND RECOVERY TARGET DNA OR RNA MOLECULES WITH A DESIRED NUCLEOTIDE SEQUENCE
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6884626B1 (en) 1998-04-27 2005-04-26 Corning Incorporated Redrawn capillary imaging reservoir
US6350618B1 (en) * 1998-04-27 2002-02-26 Corning Incorporated Redrawn capillary imaging reservoir
EP0955084B1 (en) 1998-04-27 2006-07-26 Corning Incorporated Method of depositing an array of biological samples using a redrawn capillary reservoir
CA2332510C (en) * 1998-05-18 2011-07-26 Igenex, Inc. In situ hybridization method for detecting target nucleic acid
US6762061B1 (en) 1998-07-03 2004-07-13 Corning Incorporated Redrawn capillary imaging reservoir
CA2340361A1 (en) 1998-08-21 2000-03-16 Peter C. Cheng Assays using crosslinkable immobilized nucleic acids
US6581011B1 (en) * 1999-06-23 2003-06-17 Tissueinformatics, Inc. Online database that includes indices representative of a tissue population
US6611833B1 (en) * 1999-06-23 2003-08-26 Tissueinformatics, Inc. Methods for profiling and classifying tissue using a database that includes indices representative of a tissue population
AU6920000A (en) * 1999-08-21 2001-03-19 John S. Fox High sensitivity biomolecule detection with magnetic particles
US20010016317A1 (en) * 1999-09-13 2001-08-23 Dolores M. Berger Method for providing long term stability to cells for diagnostic testing
US20040224421A1 (en) * 2000-06-15 2004-11-11 Deweerd Herman Bi-directional scanning method
EP1172445A1 (en) * 2000-07-14 2002-01-16 Praenadia GmbH A method for direct genetic analysis of target cells by using fluorescence probes
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
US20030175818A1 (en) * 2002-03-15 2003-09-18 Ross Amelia A. Devices and methods for isolating and recovering target cells
US7754155B2 (en) * 2002-03-15 2010-07-13 Ross Amelia A Devices and methods for isolating target cells
WO2003091455A1 (en) * 2002-04-23 2003-11-06 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
US20040053399A1 (en) * 2002-07-17 2004-03-18 Rudolf Gilmanshin Methods and compositions for analyzing polymers using chimeric tags
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
ES2391975T3 (es) * 2003-07-25 2012-12-03 Genvec, Inc. Vacunas a base de vector adenovírico
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
US20050221408A1 (en) * 2004-03-19 2005-10-06 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
JP2008511336A (ja) * 2004-09-01 2008-04-17 アメリカ合衆国 免疫原性が増加したアデノウイルスベクターをインビボで用いる方法
KR100760525B1 (ko) 2006-04-13 2007-10-04 김재만 병원성 미생물의 무증폭 다중 정량 검출킷트 및 검출방법
WO2009009127A2 (en) * 2007-07-12 2009-01-15 U.S. Genomics, Inc. Method and system for transferring and/or concentrating a sample
ES2688721T3 (es) 2007-11-01 2018-11-06 Astellas Pharma Inc. Polipéptidos y ácidos nucleicos inmunosupresores
GB201010580D0 (en) 2010-06-23 2010-08-11 Reckitt Benckiser Nv Machine dishwashing compositions and methods
EP3847274A4 (en) * 2018-09-05 2022-06-08 Readcoor, LLC Methods and systems for therapeutic agent analysis
JP2022535747A (ja) * 2019-05-28 2022-08-10 ケース ウエスタン リザーブ ユニバーシティ Ｄｎａのメチル化を保存するための組成物および方法
US12404538B2 (en) 2020-05-27 2025-09-02 Case Western Reserve University Compositions and methods for preserving DNA methylation
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN113741375B (zh) * 2021-09-18 2023-02-03 中国科学院近代物理研究所 重离子微孔膜专用生产终端控制系统

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JPS61502282A (ja) * 1984-06-01 1986-10-09 ナショナル・バイオメディカル・リサ−チ・ファウンデ−ション 酵素剤を用いた触媒作用による核酸交雑方法
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EP0236491A1 (en) * 1985-09-18 1987-09-16 Yale University RecA NUCLEOPROTEIN FILAMENT AND METHODS
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
DE3888653T2 (de) * 1987-12-21 1994-07-07 Applied Biosystems Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz.
JP2745816B2 (ja) * 1990-05-07 1998-04-28 ダイキン工業株式会社 ダブルｄループ形成の診断応用
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification

Also Published As

Publication number Publication date
DE69215533T2 (de) 1997-03-20
NO940746D0 (no) 1994-03-03
US5506098A (en) 1996-04-09
JP2819830B2 (ja) 1998-11-05
FI941015A7 (fi) 1994-05-03
FI941015L (fi) 1994-05-03
AU664045B2 (en) 1995-11-02
FI941015A0 (fi) 1994-03-03
AU2500792A (en) 1993-04-05
KR100245283B1 (ko) 2000-03-02
NO940746L (no) 1994-03-03
DK0614492T3 (da) 1997-06-02
EP0614492A1 (en) 1994-09-14
DE69215533D1 (de) 1997-01-09
CA2116214A1 (en) 1993-03-05
US5719023A (en) 1998-02-17
ATE145674T1 (de) 1996-12-15
JPH06510200A (ja) 1994-11-17
CA2116214C (en) 1998-12-22
EP0614492B1 (en) 1996-11-27
WO1993005177A1 (en) 1993-03-18

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