ES2101867T3 - Aplicaciones diagnosticas de la formacion de lazos d dobles. - Google Patents
Aplicaciones diagnosticas de la formacion de lazos d dobles.Info
- Publication number
- ES2101867T3 ES2101867T3 ES92918877T ES92918877T ES2101867T3 ES 2101867 T3 ES2101867 T3 ES 2101867T3 ES 92918877 T ES92918877 T ES 92918877T ES 92918877 T ES92918877 T ES 92918877T ES 2101867 T3 ES2101867 T3 ES 2101867T3
- Authority
- ES
- Spain
- Prior art keywords
- double
- formation
- dna
- diagnostic applications
- ties
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Stabilization Of Oscillater, Synchronisation, Frequency Synthesizers (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
EN LA PRESENTE INVENCION SE DESCRIBE LA FORMACION DE COMPLEJOS DE SONDA DE TRENZADO DOBLE CATALIZADA CON UNA PROTEINA RECA:ADN OBJETIVO LINEAL DUPLEX QUE SON ESTABLES A LA DESPROTEINIZACION. SE PRESENTAN LOS USOS DE ESTE COMPLEJO DE SONDA:OBJETIVO ESTABLE EN SISTEMAS DE DIAGNOSTICO/DETECCION DEL ADN EN REACCIONES DE HIBRIDACION DEL ADN IN VITRO E IN SITU. LOS COMPLEJOS SONDA:OBJETIVO SON UTILES TAMBIEN PARA APLICACIONES DE DIAGNOSTICO EN REACCIONES DE AMPLIFICACION DEL ADN FACILITADAS POR LA PROTEINA RECA.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/520,321 US5223414A (en) | 1990-05-07 | 1990-05-07 | Process for nucleic acid hybridization and amplification |
| US07/755,462 US5273881A (en) | 1990-05-07 | 1991-09-04 | Diagnostic applications of double D-loop formation |
| US91079192A | 1992-07-09 | 1992-07-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2101867T3 true ES2101867T3 (es) | 1997-07-16 |
Family
ID=27414753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92918877T Expired - Lifetime ES2101867T3 (es) | 1990-05-07 | 1992-09-04 | Aplicaciones diagnosticas de la formacion de lazos d dobles. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5670316A (es) |
| EP (1) | EP0612352B1 (es) |
| JP (1) | JP2745816B2 (es) |
| KR (1) | KR100245284B1 (es) |
| AT (1) | ATE153707T1 (es) |
| AU (1) | AU661505B2 (es) |
| CA (1) | CA2116215C (es) |
| DE (1) | DE69220084T2 (es) |
| DK (1) | DK0612352T3 (es) |
| ES (1) | ES2101867T3 (es) |
| FI (1) | FI941016A7 (es) |
| NO (1) | NO313557B1 (es) |
| WO (1) | WO1993005178A1 (es) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5506098A (en) * | 1991-09-04 | 1996-04-09 | Daikin Industries, Ltd. | In situ hybridization method |
| AU4115693A (en) * | 1992-04-24 | 1993-11-29 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
| US5929043A (en) * | 1995-01-31 | 1999-07-27 | Pangene Corporation | Recombinase mediated DNA therapies |
| FR2737502B1 (fr) * | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| CA2263815A1 (en) | 1996-08-29 | 1998-03-05 | Koji Kigawa | Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase |
| JP3891620B2 (ja) * | 1996-11-14 | 2007-03-14 | 株式会社アイシン・コスモス研究所 | ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法 |
| US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
| US6342350B1 (en) * | 1997-09-05 | 2002-01-29 | The General Hospital Corporation | Alpha-2-macroglobulin diagnostic test |
| WO1999037755A2 (en) * | 1997-12-11 | 1999-07-29 | Pangene Corporation | The use of consensus sequences for targeted homologous gene isolation and recombination in gene families |
| WO1999050446A1 (de) * | 1998-04-01 | 1999-10-07 | Wolfrum Juergen | Verfahren und vorrichtung zur quantifizierung von dna und rna |
| US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
| US6541227B1 (en) * | 1998-10-12 | 2003-04-01 | Aisin Seiki Kabushiki Kaisha | Preparation of labeled DNA |
| JP4265028B2 (ja) * | 1999-04-14 | 2009-05-20 | アイシン精機株式会社 | 二本鎖dnaの特異的切断方法およびそのためのキット |
| US6977295B2 (en) | 1999-04-21 | 2005-12-20 | Invitrogen Corporation | Locked nucleic acid hybrids and methods of use |
| JP2003517842A (ja) * | 1999-12-21 | 2003-06-03 | オーソ−クリニカル・ダイアグノスティックス・インコーポレイテッド | 核酸の検出方法 |
| CA2397357A1 (en) * | 1999-12-28 | 2001-07-05 | David A. Zarling | Recombinase mediated gene chip detection |
| WO2002077286A1 (en) * | 2001-02-21 | 2002-10-03 | Gene Check, Inc. | Mutation detection using muts and reca |
| EP1414840A4 (en) * | 2001-03-27 | 2005-04-13 | Univ Delaware | GENOMICS APPLICATIONS FOR MODIFIED OLIGONUCLEOTIDES |
| CA2444649C (en) | 2001-04-20 | 2012-10-02 | The Penn State Research Foundation | Methods for nucleic acid manipulation |
| US7468244B2 (en) * | 2001-09-27 | 2008-12-23 | University Of Delaware | Polymorphism detection and separation |
| AU2002341898A2 (en) * | 2001-09-28 | 2003-04-07 | University Of Delaware | Polymorphism detection and separation |
| US7244562B2 (en) * | 2001-11-01 | 2007-07-17 | Gene Check, Inc. | RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7270981B2 (en) * | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| WO2003100095A1 (en) * | 2002-05-08 | 2003-12-04 | Arkray, Inc. | Method of detecting target nucleic acid |
| EP1543155A4 (en) * | 2002-07-17 | 2006-08-16 | Us Genomics Inc | METHOD AND COMPOSITIONS FOR THE ANALYSIS OF POLYMERS USING CHIMERIC TAGS |
| EP1613768A4 (en) * | 2003-03-11 | 2006-05-31 | Gene Check Inc | RECA-SUPPORTED ALLELSPECIFIC OLIGONUCLEOTIDE EXTENSION PROCESS |
| US20050123944A1 (en) * | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
| WO2005017205A2 (en) * | 2003-08-04 | 2005-02-24 | U. S. Genomics, Inc. | Nucleic acid mapping using linear analysis |
| EP3985125A1 (en) * | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| JP2006055065A (ja) * | 2004-08-19 | 2006-03-02 | Toyo Kohan Co Ltd | 非対称pcrを用いた核酸の検出方法、ポリヌクレオチドプローブ、それを固定するプローブ固定化担体およびキット |
| WO2006051988A1 (ja) * | 2004-11-15 | 2006-05-18 | Riken | 核酸の増幅方法 |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| WO2007037676A1 (en) * | 2005-09-29 | 2007-04-05 | Keygene N.V. | Method and means for targeted nucleotide exchange |
| US20080220421A1 (en) * | 2006-02-27 | 2008-09-11 | Salinas Frank G | Isothermal screening for nucleic acids associated with diseases and conditions of the gi tract |
| US20080003600A1 (en) * | 2006-02-27 | 2008-01-03 | Salinas Frank G | Isothermal screening of breast cancer related nucleic acid |
| US20090061413A1 (en) * | 2006-02-27 | 2009-03-05 | Salinas Frank G | Isothermal screening of hiv-1 related nucleic acids |
| US20070202522A1 (en) * | 2006-02-27 | 2007-08-30 | Salinas Frank G | Isothermal screening of tumor cell related nucleic acids |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質 |
| EP2438196B1 (en) | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| AU2011224534B2 (en) | 2010-03-08 | 2014-10-09 | Dana-Farber Cancer Institute, Inc. | Full COLD-PCR enrichment with reference blocking sequence |
| EP2691541B1 (en) | 2011-03-31 | 2017-10-18 | Dana-Farber Cancer Institute, Inc. | Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences |
| EP2694666B1 (en) | 2011-04-07 | 2018-05-23 | Alere San Diego, Inc. | Monitoring recombinase polymerase amplification mixtures |
| EP3461910B1 (en) | 2012-04-19 | 2020-08-26 | Life Technologies Corporation | Nucleic acid amplification |
| SG10201802883UA (en) | 2012-04-19 | 2018-05-30 | Life Technologies Corp | Nucleic acid amplification |
| WO2014020137A1 (en) * | 2012-08-02 | 2014-02-06 | Qiagen Gmbh | Recombinase mediated targeted dna enrichment for next generation sequencing |
| EP2895620B1 (en) | 2012-09-11 | 2017-08-02 | Life Technologies Corporation | Nucleic acid amplification |
| WO2014043143A1 (en) | 2012-09-11 | 2014-03-20 | Life Technologies Corporation | Nucleic acid amplification |
| EP3102691B1 (en) | 2014-02-03 | 2019-09-11 | Thermo Fisher Scientific Baltics UAB | Method for controlled dna fragmentation |
| WO2015191815A1 (en) | 2014-06-13 | 2015-12-17 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| EP3307908B1 (en) | 2015-06-09 | 2019-09-11 | Life Technologies Corporation | Methods for molecular tagging |
| CA2990846C (en) * | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| US20180363037A1 (en) | 2015-12-09 | 2018-12-20 | Life Technologies Corporation | Detection and quantification of nucleic acid molecules associated with a surface |
| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| WO2018071522A1 (en) | 2016-10-11 | 2018-04-19 | Life Technologies Corporation | Rapid amplification of nucleic acids |
| WO2018111835A1 (en) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| GB2578038B (en) | 2017-06-16 | 2022-11-23 | Life Technologies Corp | Control nucleic acids, and compositions, kits, and uses thereof |
| EP3645710A1 (en) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| DE102017114537A1 (de) * | 2017-06-29 | 2019-01-03 | Endress+Hauser Conducta Gmbh+Co. Kg | Sensormembran, Sensorkappe und optischer Sensor |
| EP4310196A3 (en) | 2017-11-07 | 2024-04-24 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| EP3963103B1 (en) | 2019-05-03 | 2024-02-28 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| JP7675700B2 (ja) | 2019-08-21 | 2025-05-13 | ライフ テクノロジーズ コーポレーション | シーケンシングのためのシステムおよび方法 |
| EP4243986A1 (en) | 2020-11-16 | 2023-09-20 | Life Technologies Corporation | System and method for sequencing |
| NL2033124B1 (en) | 2022-09-23 | 2024-03-29 | Rapidemic B V | Methods and device for multiple-label nucleic acid amplification and detection |
| WO2025144744A1 (en) | 2023-12-29 | 2025-07-03 | Life Technologies Corporation | Enrichment of clonal substrates |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| CA1254114A (en) * | 1984-05-07 | 1989-05-16 | Allied Corporation | Displacement polynucleotide assay employing recombination protein and diagnostic kit |
| WO1985005685A1 (en) * | 1984-06-01 | 1985-12-19 | National Biomedical Research Foundation | Catalyzed nucleic acid hybridization using enzymatic reagent |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| EP0236491A1 (en) * | 1985-09-18 | 1987-09-16 | Yale University | RecA NUCLEOPROTEIN FILAMENT AND METHODS |
| US4888274A (en) * | 1985-09-18 | 1989-12-19 | Yale University | RecA nucleoprotein filament and methods |
| AU2735988A (en) * | 1987-12-21 | 1989-07-13 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| DE3888653T2 (de) * | 1987-12-21 | 1994-07-07 | Applied Biosystems | Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz. |
| US5223414A (en) * | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5273881A (en) * | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
| US5506098A (en) * | 1991-09-04 | 1996-04-09 | Daikin Industries, Ltd. | In situ hybridization method |
-
1992
- 1992-09-04 AT AT92918877T patent/ATE153707T1/de not_active IP Right Cessation
- 1992-09-04 JP JP5505113A patent/JP2745816B2/ja not_active Expired - Fee Related
- 1992-09-04 DE DE69220084T patent/DE69220084T2/de not_active Expired - Fee Related
- 1992-09-04 KR KR1019940700724A patent/KR100245284B1/ko not_active Expired - Fee Related
- 1992-09-04 CA CA002116215A patent/CA2116215C/en not_active Expired - Fee Related
- 1992-09-04 EP EP92918877A patent/EP0612352B1/en not_active Expired - Lifetime
- 1992-09-04 ES ES92918877T patent/ES2101867T3/es not_active Expired - Lifetime
- 1992-09-04 DK DK92918877.9T patent/DK0612352T3/da active
- 1992-09-04 WO PCT/JP1992/001135 patent/WO1993005178A1/en not_active Ceased
- 1992-09-04 FI FI941016A patent/FI941016A7/fi unknown
- 1992-09-04 US US08/199,317 patent/US5670316A/en not_active Expired - Fee Related
- 1992-09-04 AU AU25401/92A patent/AU661505B2/en not_active Ceased
-
1994
- 1994-03-03 NO NO19940744A patent/NO313557B1/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0612352B1 (en) | 1997-05-28 |
| AU661505B2 (en) | 1995-07-27 |
| DE69220084T2 (de) | 1997-09-11 |
| JP2745816B2 (ja) | 1998-04-28 |
| US5670316A (en) | 1997-09-23 |
| CA2116215C (en) | 1998-12-22 |
| NO940744D0 (no) | 1994-03-03 |
| AU2540192A (en) | 1993-04-05 |
| EP0612352A1 (en) | 1994-08-31 |
| KR100245284B1 (ko) | 2000-03-02 |
| NO313557B1 (no) | 2002-10-21 |
| FI941016L (fi) | 1994-05-03 |
| JPH06510201A (ja) | 1994-11-17 |
| ATE153707T1 (de) | 1997-06-15 |
| NO940744L (es) | 1994-05-02 |
| CA2116215A1 (en) | 1993-03-05 |
| DE69220084D1 (de) | 1997-07-03 |
| FI941016A7 (fi) | 1994-05-03 |
| FI941016A0 (fi) | 1994-03-03 |
| WO1993005178A1 (en) | 1993-03-18 |
| DK0612352T3 (da) | 1997-06-30 |
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Legal Events
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| FG2A | Definitive protection |
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