ES2103795T3 - Mejora de la captura de un acido nucleico diana mediante el uso de oligonucleotidos unidos covalentemente a polimeros. - Google Patents

Mejora de la captura de un acido nucleico diana mediante el uso de oligonucleotidos unidos covalentemente a polimeros.

Info

Publication number
ES2103795T3
ES2103795T3 ES91902823T ES91902823T ES2103795T3 ES 2103795 T3 ES2103795 T3 ES 2103795T3 ES 91902823 T ES91902823 T ES 91902823T ES 91902823 T ES91902823 T ES 91902823T ES 2103795 T3 ES2103795 T3 ES 2103795T3
Authority
ES
Spain
Prior art keywords
nucleic acid
capture
polymer
polymers
united
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91902823T
Other languages
English (en)
Inventor
Ness Jeffrey Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
MicroProbe Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MicroProbe Corp filed Critical MicroProbe Corp
Application granted granted Critical
Publication of ES2103795T3 publication Critical patent/ES2103795T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

ESTA INVENCION ESTA RELACIONADA CON METODOS PARA DETECTAR SECUENCIAS POLINUCLEOTIDAS UTILIZANDO PROCEDIMIENTOS DE HIBRIDIZACION DE ACIDO NUCLEICO. EN PARTICULAR, LA INVENCION SE REFIERE AL USO DE OLIGONUCLEOTIDOS COVALENTEMENTE UNIDOS A UN POLIMERO PARA INCREMENTAR LA VELOCIDAD Y EL ALCANCE DE CAPTURA DE ACIDO NUCLEICO BLANCO ESPECIFICO CONTENIDO EN UNA MUESTRA BIOLOGICA. EN UN METODO PREFERIDO, SE EXPONE EL ACIDO NUCLEICO BLANCO DE LA MUESTRA A UN POLIMERO DE CAPTURA POLINUCLEOTIDA QUE TIENE MULTIPLES SECUENCIAS OLIGONUCLEOTIDAS COMPLEMENTARIAS A LAS SECUENCIAS EN EL BLANCO. TRAS LA CAPTURA DE LOS POLINUCLEOTIDOS BLANCO, EL POLIMERO SE INMOVILIZA (COMPLEJANTADO) EN UN SOPORTE SOLIDO A TRAVES DE UNA COMBINACION LIGANTE/RECEPTORA.
ES91902823T 1989-12-04 1990-11-29 Mejora de la captura de un acido nucleico diana mediante el uso de oligonucleotidos unidos covalentemente a polimeros. Expired - Lifetime ES2103795T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44487289A 1989-12-04 1989-12-04

Publications (1)

Publication Number Publication Date
ES2103795T3 true ES2103795T3 (es) 1997-10-01

Family

ID=23766693

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91902823T Expired - Lifetime ES2103795T3 (es) 1989-12-04 1990-11-29 Mejora de la captura de un acido nucleico diana mediante el uso de oligonucleotidos unidos covalentemente a polimeros.

Country Status (5)

Country Link
EP (1) EP0504321B1 (es)
AT (1) ATE152777T1 (es)
DE (1) DE69030672T2 (es)
ES (1) ES2103795T3 (es)
WO (1) WO1991008307A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
FR2679255B1 (fr) * 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
EP0656065A1 (en) * 1991-10-31 1995-06-07 University Of Pittsburgh Reverse dot blot hybridization using tandem head-to-tail monomers containing probes synthesized by staggered complementary primers
FR2701961B1 (fr) * 1993-02-24 1995-04-21 Bio Merieux Procédé de destabilisation d'une structure secondaire intracaténaire d'un polynucléotide simple brin, et de capture dudit nucléotide.
FR2703359B1 (fr) * 1993-03-31 1995-06-23 Cis Bio Int Copolymère nucléotide(s)/polymère conducteur électronique ; son procédé de préparation et son utilisation .
FR2707010B1 (es) * 1993-06-25 1995-09-29 Bio Merieux
FR2707296B1 (fr) * 1993-07-09 1995-09-29 Genset Sa Procédé de synthèse d'acides nucléiques sur support solide et composés utiles notamment comme support solide dans ledit procédé.
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
DE19537952A1 (de) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU714486B2 (en) 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
FR2746413B1 (fr) * 1996-03-19 1998-04-24 Bio Merieux Detection d'une sequence nucleotidique avec amplification de signal
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
CA2276462C (en) 1996-12-31 2007-06-12 High Throughput Genomics, Inc. Multiplexed molecular analysis system apparatus and method
DE69836012T2 (de) 1997-05-02 2007-04-05 Gen-Probe Inc., San Diego Zwei-schritt hybridisierung und einfang von einem polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
WO1998051823A1 (en) 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
EP1027458A2 (en) 1997-11-06 2000-08-16 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
US6238869B1 (en) * 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US20030096232A1 (en) 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6531278B1 (en) * 1998-01-14 2003-03-11 Utah State University Ligand-DNA composition for capture and detection of contaminants on a solid surface
CA2331767A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
AU763890B2 (en) 1998-09-15 2003-07-31 Yale University Artificial long terminal repeat vectors
AU6412799A (en) 1998-10-05 2000-04-26 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
FR2802550B1 (fr) * 1999-12-17 2004-03-05 Centre Nat Rech Scient Biopuces, preparation et utilisations
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU7172201A (en) 2000-06-30 2002-01-14 Molecular Staging Inc Signal amplification with lollipop probes
GB0016813D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016836D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20050112636A1 (en) * 2003-09-23 2005-05-26 Atom Sciences Polymeric nucleic acid hybridization probes
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1934339B1 (en) 2005-10-06 2011-12-14 Lucigen Corporation Thermostable viral polymerases and methods of use
ES2990227T3 (es) 2008-01-17 2024-11-29 Sequenom Inc Composiciones y procedimientos de análisis de ácido nucleico de secuencia única
US8501490B2 (en) 2010-01-08 2013-08-06 The Regents Of The University Of California Bioassays based on polymeric sequence probe
EP3155426B1 (en) 2014-06-13 2023-07-19 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
WO2021243271A2 (en) 2020-05-29 2021-12-02 Front Range Biosciences, Inc. Methods and compositions for pathogen detection in plants
US20230002779A1 (en) 2020-06-29 2023-01-05 Front Range Biosciences, Inc. Characterization of plant cultivars based on terpene synthase gene profiles
CN114591944B (zh) * 2021-12-21 2025-04-04 杭州康大晨星医学科技有限公司 一种高灵敏度hpv病毒检测试剂盒

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays

Also Published As

Publication number Publication date
ATE152777T1 (de) 1997-05-15
EP0504321B1 (en) 1997-05-07
EP0504321A1 (en) 1992-09-23
DE69030672D1 (de) 1997-06-12
EP0504321A4 (en) 1993-07-14
WO1991008307A1 (en) 1991-06-13
DE69030672T2 (de) 1997-11-13

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