ATE152777T1 - Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind - Google Patents

Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind

Info

Publication number
ATE152777T1
ATE152777T1 AT91902823T AT91902823T ATE152777T1 AT E152777 T1 ATE152777 T1 AT E152777T1 AT 91902823 T AT91902823 T AT 91902823T AT 91902823 T AT91902823 T AT 91902823T AT E152777 T1 ATE152777 T1 AT E152777T1
Authority
AT
Austria
Prior art keywords
target nucleic
nucleic acid
polymers
capture
polymer
Prior art date
Application number
AT91902823T
Other languages
English (en)
Inventor
Ness Jeffrey Van
Original Assignee
Microprobe Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microprobe Corp filed Critical Microprobe Corp
Application granted granted Critical
Publication of ATE152777T1 publication Critical patent/ATE152777T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT91902823T 1989-12-04 1990-11-29 Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind ATE152777T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44487289A 1989-12-04 1989-12-04

Publications (1)

Publication Number Publication Date
ATE152777T1 true ATE152777T1 (de) 1997-05-15

Family

ID=23766693

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902823T ATE152777T1 (de) 1989-12-04 1990-11-29 Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind

Country Status (5)

Country Link
EP (1) EP0504321B1 (de)
AT (1) ATE152777T1 (de)
DE (1) DE69030672T2 (de)
ES (1) ES2103795T3 (de)
WO (1) WO1991008307A1 (de)

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US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
FR2679255B1 (fr) * 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
JPH07500734A (ja) * 1991-10-31 1995-01-26 ユニヴァーシティ　オブ　ピッツバーグ オリゴヌクレオチドプローブのポリマーを用いる核酸配列の検出方法
FR2701961B1 (fr) * 1993-02-24 1995-04-21 Bio Merieux Procédé de destabilisation d'une structure secondaire intracaténaire d'un polynucléotide simple brin, et de capture dudit nucléotide.
FR2703359B1 (fr) 1993-03-31 1995-06-23 Cis Bio Int Copolymère nucléotide(s)/polymère conducteur électronique ; son procédé de préparation et son utilisation .
FR2707010B1 (de) * 1993-06-25 1995-09-29 Bio Merieux
FR2707296B1 (fr) * 1993-07-09 1995-09-29 Genset Sa Procédé de synthèse d'acides nucléiques sur support solide et composés utiles notamment comme support solide dans ledit procédé.
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
DE19537952A1 (de) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
DK0862656T3 (da) 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
FR2746413B1 (fr) * 1996-03-19 1998-04-24 Bio Merieux Detection d'une sequence nucleotidique avec amplification de signal
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
CA2276462C (en) 1996-12-31 2007-06-12 High Throughput Genomics, Inc. Multiplexed molecular analysis system apparatus and method
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
EP0975807B1 (de) 1997-05-02 2006-09-27 Gen-Probe Incorporated Zwei-schritt hybridisierung und einfang von einem polynukleotid
ATE335845T1 (de) 1997-05-16 2006-09-15 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
AU1384299A (en) 1997-11-06 1999-05-31 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
US20030096232A1 (en) 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6238869B1 (en) * 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6531278B1 (en) * 1998-01-14 2003-03-11 Utah State University Ligand-DNA composition for capture and detection of contaminants on a solid surface
WO1999066078A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
EP1112385A1 (de) 1998-09-15 2001-07-04 Yale University Künstliche vektoren mit langen wiederholungssequenzen
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6261779B1 (en) 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
FR2802550B1 (fr) * 1999-12-17 2004-03-05 Centre Nat Rech Scient Biopuces, preparation et utilisations
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
CA2411794A1 (en) 2000-06-30 2002-01-10 Molecular Staging, Inc. Signal amplification with lollipop probes
GB0016813D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016836D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
EP1687445A4 (de) * 2003-09-23 2007-03-28 Atom Sciences Inc Polymere nukleinsäurehybridisierungssonden
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
CA2624324A1 (en) 2005-10-06 2007-04-19 Lucigen Corporation Thermostable viral polymerases and methods of use
WO2009091934A1 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
WO2011084168A1 (en) 2010-01-08 2011-07-14 The Regents Of The University Of California Bioassays based on polymeric sequence probes
WO2015188839A2 (en) 2014-06-13 2015-12-17 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
CA3185019A1 (en) 2020-05-29 2021-12-02 Front Range Biosciences, Inc. Methods and compositions for pathogen detection in plants
US20230002779A1 (en) 2020-06-29 2023-01-05 Front Range Biosciences, Inc. Characterization of plant cultivars based on terpene synthase gene profiles
CN114591944B (zh) * 2021-12-21 2025-04-04 杭州康大晨星医学科技有限公司 一种高灵敏度hpv病毒检测试剂盒

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays

Also Published As

Publication number Publication date
EP0504321A4 (en) 1993-07-14
WO1991008307A1 (en) 1991-06-13
EP0504321B1 (de) 1997-05-07
ES2103795T3 (es) 1997-10-01
DE69030672D1 (de) 1997-06-12
EP0504321A1 (de) 1992-09-23
DE69030672T2 (de) 1997-11-13

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