ES2103803T3 - Ensayo inmunocromatografico y metodo para utilizar el mismo. - Google Patents
Ensayo inmunocromatografico y metodo para utilizar el mismo.Info
- Publication number
- ES2103803T3 ES2103803T3 ES91904867T ES91904867T ES2103803T3 ES 2103803 T3 ES2103803 T3 ES 2103803T3 ES 91904867 T ES91904867 T ES 91904867T ES 91904867 T ES91904867 T ES 91904867T ES 2103803 T3 ES2103803 T3 ES 2103803T3
- Authority
- ES
- Spain
- Prior art keywords
- tests
- immunocromatographic
- test
- lat
- latex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/814—Pregnancy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
LA INVENCION EXPUESTA SE REFIERE AL CAMPO DE PRUEBAS DE UN SOLO PASO Y A LAS PRUEBAS DE AGLUTINACION DE LATEX ("LAT"), Y PROPORCIONA UN METODO PERFECCIONADO DE PREPARACION Y USO DE PRUEBAS DE INMUNIDAD DE UN PASO, ASI COMO DE METODOS PERFECCIONADOS DE PREPARACION DE PARTICULAS DE LATEX CUBIERTAS PARA UTILIZAR EN PRUEBAS DE DIAGNOSTICO Y, ESPECIALMENTE, EN PRUEBAS INMUNOCROMATOGRAFICAS.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/476,788 US5096837A (en) | 1990-02-08 | 1990-02-08 | Immunochromatographic assay and method of using same |
| US476788 | 1990-02-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2103803T3 true ES2103803T3 (es) | 1997-10-01 |
| ES2103803T5 ES2103803T5 (es) | 2007-12-16 |
Family
ID=23893251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91904867T Expired - Lifetime ES2103803T5 (es) | 1990-02-08 | 1991-02-08 | Metodo inmunocromatografico. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5096837A (es) |
| EP (1) | EP0466914B2 (es) |
| JP (1) | JP3317699B2 (es) |
| KR (1) | KR920701471A (es) |
| AT (1) | ATE153701T1 (es) |
| AU (1) | AU649022B2 (es) |
| CA (1) | CA2049919C (es) |
| DE (1) | DE69126248T3 (es) |
| ES (1) | ES2103803T5 (es) |
| WO (1) | WO1991012336A1 (es) |
Families Citing this family (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6168956B1 (en) | 1991-05-29 | 2001-01-02 | Beckman Coulter, Inc. | Multiple component chromatographic assay device |
| US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
| US5998220A (en) | 1991-05-29 | 1999-12-07 | Beckman Coulter, Inc. | Opposable-element assay devices, kits, and methods employing them |
| AU670108B2 (en) | 1992-09-11 | 1996-07-04 | Becton Dickinson & Company | Improved antibodies to plasmodium falciparum |
| US5837546A (en) * | 1993-08-24 | 1998-11-17 | Metrika, Inc. | Electronic assay device and method |
| DE4434093A1 (de) * | 1994-09-23 | 1996-03-28 | Boehringer Mannheim Gmbh | Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz |
| US5753517A (en) * | 1996-03-29 | 1998-05-19 | University Of British Columbia | Quantitative immunochromatographic assays |
| US5968839A (en) * | 1996-05-13 | 1999-10-19 | Metrika, Inc. | Method and device producing a predetermined distribution of detectable change in assays |
| US6165798A (en) * | 1996-10-10 | 2000-12-26 | University Of British Columbia | Optical quantification of analytes in membranes |
| CA2269097C (en) | 1996-11-08 | 2007-01-09 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Synthesis and purification of hepatitis c virus-like particles |
| US5879951A (en) | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
| US20050101032A1 (en) * | 1997-02-10 | 2005-05-12 | Metrika, Inc. | Assay device, composition, and method of optimizing assay sensitivity |
| US5939252A (en) | 1997-05-09 | 1999-08-17 | Lennon; Donald J. | Detachable-element assay device |
| US6194222B1 (en) * | 1998-01-05 | 2001-02-27 | Biosite Diagnostics, Inc. | Methods for monitoring the status of assays and immunoassays |
| US20060019404A1 (en) * | 1998-05-06 | 2006-01-26 | Blatt Joel M | Quantitative assay with extended dynamic range |
| US6136610A (en) | 1998-11-23 | 2000-10-24 | Praxsys Biosystems, Inc. | Method and apparatus for performing a lateral flow assay |
| US6790661B1 (en) * | 1999-07-16 | 2004-09-14 | Verax Biomedical, Inc. | System for detecting bacteria in blood, blood products, and fluids of tissues |
| US6316205B1 (en) | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
| US6699722B2 (en) | 2000-04-14 | 2004-03-02 | A-Fem Medical Corporation | Positive detection lateral-flow apparatus and method for small and large analytes |
| US6767710B2 (en) * | 2001-03-30 | 2004-07-27 | Praxsys Biosystems, Llc | Prewetting stop flow test strip |
| JP2004538453A (ja) * | 2001-07-18 | 2004-12-24 | スーリャン ゾー, | 全細胞を含有するサンプルに対するラテラルフローアッセイのための試験片 |
| US7300633B2 (en) | 2001-07-25 | 2007-11-27 | Oakville Hong Kong Company Limited | Specimen collection container |
| US7270959B2 (en) | 2001-07-25 | 2007-09-18 | Oakville Hong Kong Company Limited | Specimen collection container |
| US7456025B2 (en) * | 2001-08-28 | 2008-11-25 | Porex Corporation | Sintered polymer membrane for analyte detection device |
| US8481334B1 (en) | 2001-11-06 | 2013-07-09 | Charm Sciences, Inc. | Method of attaching a ligand to a solid support |
| US20030175991A1 (en) * | 2002-03-18 | 2003-09-18 | Ho Winston Z. | Optically-assisted high precision pregnancy progress monitoring |
| US7560272B2 (en) * | 2003-01-04 | 2009-07-14 | Inverness Medical Switzerland Gmbh | Specimen collection and assay container |
| US7517495B2 (en) * | 2003-08-25 | 2009-04-14 | Inverness Medical Switzerland Gmbh | Biological specimen collection and analysis system |
| WO2005031355A1 (en) * | 2003-09-22 | 2005-04-07 | Quidel Corporation | Devices for the detection of multiple analytes in a sample |
| US7150995B2 (en) * | 2004-01-16 | 2006-12-19 | Metrika, Inc. | Methods and systems for point of care bodily fluid analysis |
| US20050227370A1 (en) * | 2004-03-08 | 2005-10-13 | Ramel Urs A | Body fluid analyte meter & cartridge system for performing combined general chemical and specific binding assays |
| US7632687B2 (en) * | 2004-03-23 | 2009-12-15 | Quidel Corporation | Hybrid phase lateral flow assay |
| US8003407B2 (en) * | 2004-07-29 | 2011-08-23 | Relia Diagnostic Systems, Llc | Lateral flow system and assay |
| US8475735B2 (en) * | 2004-11-01 | 2013-07-02 | Uma Mahesh Babu | Disposable immunodiagnostic test system |
| DK2645106T4 (da) | 2005-04-04 | 2024-12-02 | Biogen Ma Inc | Fremgangsmåder til evaluering af et immunrespons på et terapeutisk middel |
| US9056291B2 (en) | 2005-11-30 | 2015-06-16 | Micronics, Inc. | Microfluidic reactor system |
| US7763453B2 (en) | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
| US7871568B2 (en) * | 2006-01-23 | 2011-01-18 | Quidel Corporation | Rapid test apparatus |
| US7794656B2 (en) * | 2006-01-23 | 2010-09-14 | Quidel Corporation | Device for handling and analysis of a biological sample |
| US20070207141A1 (en) | 2006-02-28 | 2007-09-06 | Ivan Lieberburg | Methods of treating inflammatory and autoimmune diseases with natalizumab |
| MX2008011176A (es) | 2006-03-03 | 2008-09-10 | Elan Pharm Inc | Metodos para tratar padecimientos inflamatorios y autoinmunes con natalizumab. |
| US7527765B2 (en) | 2006-04-11 | 2009-05-05 | Harrogate Holdings | Consumer food testing device |
| AU2007265628B2 (en) | 2006-06-23 | 2012-12-06 | Revvity Health Sciences, Inc. | Methods and devices for microfluidic point-of-care immunoassays |
| WO2008021954A2 (en) * | 2006-08-09 | 2008-02-21 | Biogen Idec Ma Inc. | Method for distribution of a drug |
| WO2008061130A2 (en) * | 2006-11-15 | 2008-05-22 | Ucp Biosciences, Inc. | An improved collecting and testing device and method of use |
| US9086408B2 (en) | 2007-04-30 | 2015-07-21 | Nexus Dx, Inc. | Multianalyte assay |
| WO2008154813A1 (fr) | 2007-06-15 | 2008-12-24 | Xiamen University | Anticorps monoclonaux se liant à l'hémagglutinine du virus de la grippe aviaire du sous type h5 et ses utilisations |
| US8692873B2 (en) | 2009-01-15 | 2014-04-08 | Alverix, Inc. | Video-frame data receiver with low frame capture rate |
| US8422740B2 (en) | 2009-01-15 | 2013-04-16 | Scott Dylewski | Methods for determining a liquid front position on a test strip |
| US20120015350A1 (en) * | 2009-03-10 | 2012-01-19 | Northwestern University | Lateral flow strip and uses thereof |
| WO2010107654A2 (en) | 2009-03-16 | 2010-09-23 | Abaxis, Inc. | Split flow device for analyses of specific-binding partners |
| US20100267049A1 (en) * | 2009-04-15 | 2010-10-21 | Rutter William J | Diagnostic devices and related methods |
| MX2012004105A (es) | 2009-10-09 | 2012-09-07 | Invisible Sentinel Inc | Dispositivo para deteccion de antigenos y usos del mismo. |
| ES2666372T3 (es) | 2009-10-11 | 2018-05-04 | Biogen Ma Inc. | Ensayos relacionados con anti-VLA-4 |
| US20110151435A1 (en) | 2009-12-17 | 2011-06-23 | Abaxis, Inc. | Novel assays for detecting analytes in samples and kits and compositions related thereto |
| US11287423B2 (en) | 2010-01-11 | 2022-03-29 | Biogen Ma Inc. | Assay for JC virus antibodies |
| CN102906278A (zh) | 2010-01-11 | 2013-01-30 | 比奥根艾迪克Ma公司 | 用于jc病毒抗体的测定 |
| CA2786569C (en) | 2010-01-29 | 2019-04-09 | Perkinelmer Health Sciences, Inc. | Sample-to-answer microfluidic cartridge |
| US10295472B2 (en) | 2010-05-05 | 2019-05-21 | Alverix, Inc. | Assay reader operable to scan a test strip |
| CA2805720A1 (en) | 2010-06-17 | 2011-12-22 | Abaxis, Inc. | Rotors for immunoassays |
| WO2012103511A2 (en) | 2011-01-27 | 2012-08-02 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
| DK2715352T3 (da) | 2011-05-31 | 2019-05-20 | Biogen Ma Inc | Fremgangsmåde til vurdering af risiko for pml |
| US8211715B1 (en) | 2011-11-15 | 2012-07-03 | Harrogate Holdings, Ltd. Co. | Consumer food testing device providing remote monitoring |
| CN104081210B (zh) | 2011-12-23 | 2018-11-30 | 雅培医护站股份有限公司 | 具有气动式样本致动的光学测定装置 |
| US9194859B2 (en) | 2011-12-23 | 2015-11-24 | Abbott Point Of Care Inc. | Reader devices for optical and electrochemical test devices |
| WO2013096817A2 (en) | 2011-12-23 | 2013-06-27 | Abbott Point Of Care Inc | Integrated test device for optical detection of microarrays |
| ES2742864T3 (es) | 2012-03-09 | 2020-02-17 | Invisible Sentinel Inc | Métodos y composiciones para detectar múltiples analitos con una única señal |
| CN104919035B (zh) | 2012-12-21 | 2017-08-11 | 精密公司 | 便携式荧光检测系统和微测定盒 |
| KR20150096788A (ko) | 2012-12-21 | 2015-08-25 | 마이크로닉스 인코포레이티드. | 마이크로 유체공학 용도를 위한 저탄성 막 |
| JP6498125B2 (ja) | 2012-12-21 | 2019-04-10 | マイクロニクス, インコーポレイテッド | 流体回路および関連する製造方法 |
| JP2015072249A (ja) * | 2013-03-29 | 2015-04-16 | 富士フイルム株式会社 | 被検物質の測定方法、被検物質測定キット及び被検物質測定試薬 |
| WO2014182844A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
| EP2994543B1 (en) | 2013-05-07 | 2018-08-15 | Micronics, Inc. | Device for preparation and analysis of nucleic acids |
| CA2911303C (en) | 2013-05-07 | 2021-02-16 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
| WO2014193804A1 (en) | 2013-05-28 | 2014-12-04 | Biogen Idec Ma Inc. | Method of assessing risk of pml |
| JP6118761B2 (ja) * | 2014-06-05 | 2017-04-19 | 富士フイルム株式会社 | 被検物質測定キット及び被検物質の測定方法 |
| KR102567398B1 (ko) | 2015-08-06 | 2023-08-17 | 리아 다이아그노스틱스, 인크. | 물 분산성 분석 |
| US12235265B2 (en) * | 2016-06-09 | 2025-02-25 | Denka Company Limited | Immunochromatographic test piece and specimen adding device for extracting and measuring sugar chain antigen, and immunochromatography method using same |
| EP3980182A1 (en) | 2019-06-04 | 2022-04-13 | Abbott Toxicology Limited | Fluid specimen testing |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4169138A (en) * | 1974-05-29 | 1979-09-25 | Pharmacia Diagnostics Ab | Method for the detection of antibodies |
| CA1101330A (en) * | 1977-09-19 | 1981-05-19 | Ernst A. Fischer | Immunological material bonded to carboxylated latex polymer and process for making it |
| DE3066899D1 (en) * | 1979-01-18 | 1984-04-19 | Unilever Plc | Method for detecting and determining proteinaceous specific binding agents and materials bindable thereto, test composition and testkit therefor |
| NL8000173A (nl) * | 1980-01-11 | 1981-08-03 | Akzo Nv | Toepassing van in water dispergeerbare, hydrofobe kleurstoffen als label in immunochemische testen. |
| US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| US4351824A (en) * | 1981-02-05 | 1982-09-28 | Icl Scientific | Polystyrene latex reagents, methods of preparation, and use in immunological procedures |
| US4419453A (en) * | 1981-09-28 | 1983-12-06 | The Dow Chemical Company | Immunological agglutination assays with dyed or colored latex and kits |
| US4419435A (en) * | 1982-05-21 | 1983-12-06 | Eastman Kodak Company | Photographic products and processes employing 6-heterocyclylazo-3-pyridinol nondiffusible cyan dye-releasing compounds and precursors thereof |
| US4591570A (en) * | 1983-02-02 | 1986-05-27 | Centocor, Inc. | Matrix of antibody-coated spots for determination of antigens |
| US4496654A (en) * | 1983-04-08 | 1985-01-29 | Quidel | Detection of HCG with solid phase support having avidin coating |
| US4552839A (en) * | 1983-08-01 | 1985-11-12 | Syntex (U.S.A.) Inc. | Determination of analytes in particle-containing medium |
| US4703017C1 (en) * | 1984-02-14 | 2001-12-04 | Becton Dickinson Co | Solid phase assay with visual readout |
| US4632901A (en) * | 1984-05-11 | 1986-12-30 | Hybritech Incorporated | Method and apparatus for immunoassays |
| JPS60256057A (ja) * | 1984-06-01 | 1985-12-17 | Dai Ichi Pure Chem Co Ltd | 免疫学的測定法 |
| DE3856421T2 (de) * | 1987-04-27 | 2000-12-14 | Unilever Nv | Spezifische Bindungstestverfahren |
| US4855240A (en) * | 1987-05-13 | 1989-08-08 | Becton Dickinson And Company | Solid phase assay employing capillary flow |
| US4912034A (en) * | 1987-09-21 | 1990-03-27 | Biogenex Laboratories | Immunoassay test device and method |
| CA1312544C (en) * | 1987-12-21 | 1993-01-12 | James L. Wilcox | Chromatographic binding assay devices and methods |
-
1990
- 1990-02-08 US US07/476,788 patent/US5096837A/en not_active Expired - Lifetime
-
1991
- 1991-02-08 WO PCT/US1991/000896 patent/WO1991012336A1/en not_active Ceased
- 1991-02-08 AT AT91904867T patent/ATE153701T1/de not_active IP Right Cessation
- 1991-02-08 CA CA002049919A patent/CA2049919C/en not_active Expired - Fee Related
- 1991-02-08 JP JP50470691A patent/JP3317699B2/ja not_active Expired - Fee Related
- 1991-02-08 DE DE69126248T patent/DE69126248T3/de not_active Expired - Fee Related
- 1991-02-08 ES ES91904867T patent/ES2103803T5/es not_active Expired - Lifetime
- 1991-02-08 KR KR1019910701267A patent/KR920701471A/ko not_active Withdrawn
- 1991-02-08 EP EP91904867A patent/EP0466914B2/en not_active Expired - Lifetime
- 1991-02-08 AU AU73162/91A patent/AU649022B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2049919A1 (en) | 1991-08-09 |
| KR920701471A (ko) | 1992-08-11 |
| AU7316291A (en) | 1991-09-03 |
| EP0466914A1 (en) | 1992-01-22 |
| JP3317699B2 (ja) | 2002-08-26 |
| ATE153701T1 (de) | 1997-06-15 |
| AU649022B2 (en) | 1994-05-12 |
| WO1991012336A1 (en) | 1991-08-22 |
| EP0466914B2 (en) | 2007-06-06 |
| DE69126248D1 (de) | 1997-07-03 |
| DE69126248T2 (de) | 1997-09-25 |
| ES2103803T5 (es) | 2007-12-16 |
| EP0466914B1 (en) | 1997-05-28 |
| CA2049919C (en) | 1996-11-19 |
| US5096837A (en) | 1992-03-17 |
| JPH04504764A (ja) | 1992-08-20 |
| EP0466914A4 (en) | 1992-06-24 |
| DE69126248T3 (de) | 2008-01-24 |
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