ES2107451T3 - Proceso de fermentacion para la produccion de desoxirribonucleosidos de pirimidina. - Google Patents

Proceso de fermentacion para la produccion de desoxirribonucleosidos de pirimidina.

Info

Publication number
ES2107451T3
ES2107451T3 ES91901364T ES91901364T ES2107451T3 ES 2107451 T3 ES2107451 T3 ES 2107451T3 ES 91901364 T ES91901364 T ES 91901364T ES 91901364 T ES91901364 T ES 91901364T ES 2107451 T3 ES2107451 T3 ES 2107451T3
Authority
ES
Spain
Prior art keywords
production
pirimidine
deoxyribonucleosides
fermentation process
dna coding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91901364T
Other languages
English (en)
Inventor
Russell J Mcdandliss
David M Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemgen Corp
Original Assignee
Chemgen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemgen Corp filed Critical Chemgen Corp
Application granted granted Critical
Publication of ES2107451T3 publication Critical patent/ES2107451T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/385—Pyrimidine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE UTILIZA LA CODIFICACION DEL ADN PARA AL MENOS UNA ENCIMA QUE PRODUCE LA ACUMULACION DE DESOXIRRIBONUCLEASA DE PIRIMIDINA, JUNTO A MUTACIONES METABOLICAS O CODIFICACION DEL ADN HETEROLOGO PARA ENCIMAS METABOLICAS QUE AUMENTAN TAMBIEN LA PRODUCCION DE DESOXIRRIBONUCLEASAS DE PIRIMIDINA, PARA CONTROLAR LAS CELULAS CULTIVADAS PARA QUE EMITAN UNA DESOXIRRIBONUCLEASA DE PIRIMIDINA (PDN) EN CANTIDADES RECUPERABLES, PROPORCIONANDO UNA FUENTE DE FERMENTACION UTIL COMERCIALMENTE DE PDN.
ES91901364T 1989-12-08 1990-12-05 Proceso de fermentacion para la produccion de desoxirribonucleosidos de pirimidina. Expired - Lifetime ES2107451T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/448,158 US5213972A (en) 1989-12-08 1989-12-08 Fermentation process for the production of pyrimidine deoxyribonucleosides

Publications (1)

Publication Number Publication Date
ES2107451T3 true ES2107451T3 (es) 1997-12-01

Family

ID=23779229

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91901364T Expired - Lifetime ES2107451T3 (es) 1989-12-08 1990-12-05 Proceso de fermentacion para la produccion de desoxirribonucleosidos de pirimidina.

Country Status (12)

Country Link
US (1) US5213972A (es)
EP (1) EP0504279B1 (es)
JP (1) JP3032292B2 (es)
KR (1) KR0180897B1 (es)
AT (1) ATE155170T1 (es)
AU (1) AU642199B2 (es)
CA (1) CA2070826C (es)
DE (1) DE69031042T2 (es)
DK (1) DK0504279T3 (es)
ES (1) ES2107451T3 (es)
GR (1) GR3024967T3 (es)
WO (1) WO1991009130A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE173166T1 (de) * 1992-09-04 1998-11-15 Univ Saskatchewan Neue bakterielle impfstoffe unter verwendung von impfstämmen von pathogenen bakterien
FR2779446B1 (fr) * 1998-06-09 2001-07-13 Commissariat Energie Atomique Procede de synthese enzymatique de desoxyribonucleosides triphosphates uniformement marques aux isotopes stables ou radioactifs et ses applications
DE60035781T2 (de) 1999-07-01 2008-08-07 Glaxo Group Ltd., Greenford Vektoren, zellen und verfahren zur herstellung von pyrimidindeoxyribonucleosiden
EP1806405A3 (en) * 1999-07-01 2007-10-03 Glaxo Group Limited Vectors, cells and processes for pyrimidine deoxyribonucleosides production
GB0602339D0 (en) * 2006-02-06 2006-03-15 Glaxo Group Ltd Novel process
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
DE102012016716A1 (de) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
CN113755416B (zh) * 2020-06-05 2023-08-15 苏州华赛生物工程技术有限公司 具有新合成路径生产β-胸苷的重组微生物及生产β-胸苷的方法
KR102398032B1 (ko) * 2020-09-22 2022-05-19 비피진 주식회사 암 치료를 위한 5''-뉴클레오티다아제 변이체
KR102579284B1 (ko) * 2020-09-22 2023-09-18 비피진 주식회사 신규의 cd47 바인더와 폴리뉴클레오티드를 포함하는 암 치료를 위한 리포좀 복합체
CN112210577A (zh) * 2020-11-04 2021-01-12 赤峰蒙广生物科技有限公司 一种发酵法生产β-胸苷的方法
CN114410561B (zh) * 2022-01-28 2023-09-01 天津科技大学 一种生产胸苷的基因工程菌株及其构建方法与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3786771T2 (de) * 1986-12-26 1993-11-11 Takeda Chemical Industries Ltd Für inaktivierte IMP-Dehydrogenase kodierende DNS.
JPH022349A (ja) * 1988-02-17 1990-01-08 Takeda Chem Ind Ltd ピリミジンアナログ耐性化遺伝子ｄｎａおよびその用途
DE68916708T2 (de) * 1988-05-31 1994-12-01 Zeneca Ltd Herstellung eines Desoxyribonucleosids.

Also Published As

Publication number Publication date
GR3024967T3 (en) 1998-01-30
KR0180897B1 (ko) 1999-04-01
EP0504279B1 (en) 1997-07-09
JPH05504055A (ja) 1993-07-01
US5213972A (en) 1993-05-25
CA2070826C (en) 2001-10-30
KR920703827A (ko) 1992-12-18
WO1991009130A1 (en) 1991-06-27
ATE155170T1 (de) 1997-07-15
DK0504279T3 (da) 1998-03-02
EP0504279A1 (en) 1992-09-23
DE69031042D1 (de) 1997-08-14
JP3032292B2 (ja) 2000-04-10
CA2070826A1 (en) 1991-06-09
DE69031042T2 (de) 1998-02-26
AU642199B2 (en) 1993-10-14
EP0504279A4 (en) 1993-02-17
AU7037491A (en) 1991-07-18

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