ES2109983T3 - Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos. - Google Patents

Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.

Info

Publication number
ES2109983T3
ES2109983T3 ES92306396T ES92306396T ES2109983T3 ES 2109983 T3 ES2109983 T3 ES 2109983T3 ES 92306396 T ES92306396 T ES 92306396T ES 92306396 T ES92306396 T ES 92306396T ES 2109983 T3 ES2109983 T3 ES 2109983T3
Authority
ES
Spain
Prior art keywords
exo
sample
nucleic acid
acid amplification
incorporated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92306396T
Other languages
English (en)
Inventor
James L Hartley
Mark Berninger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of ES2109983T3 publication Critical patent/ES2109983T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTA UN METODO PARA INCORPORAR UN NUCLEOTIDO DE PATRON EXO (POR EJEMPLO DESOXIURIDINA EN DNA) EN LOS RAMALES DE UN PRODUCTO AMPLIFICADO QUE RESULTA DE UN PROCESO DE AMPLIFICACION DEL ACIDO NUCLEICO. UNA VEZ QUE LOS RAMALES DEL PRODUCTO HAN SIDO OBTENIDOS Y ANALIZADOS (POR EJ. MEDIANTE HIBRIDIZACION), LOS RAMALES DE PATRON EXO PUEDEN DESTRUIRSE SELECTIVAMENTE ACTUANDO SOBRE EL NUCLEOTIDO DE PATRON EXO INCORPORADO. SE DESCRIBEN DOS PROCEDIMIENTOS. EN EL PRIMERO PROCEDIMIENTO EL NUCLEOTIDO DE PATRON EXO SE INCORPORA CONDUCIENDO LA REACCION DE AMPLIFICACION EN LA PRESENCIA DE UN EXCESO DE TRIFOSFATO DE NUCLEOTIDO DE PATRON EXO. EN EL SEGUNDO PROCEDIMIENTO EL NUCLEOTIDO DE PATRON EXO SE INCORPORA CONDUCIENDO LA REACCION DE AMPLIFICACION EN LA PRESENCIA DE UN OLIGONUCLEOTIDO QUE TENGA, COMO PARTE DE SU SECUENCIA, UNO O MAS NUCLEOTIDOS DE PATRON EXO.
ES92306396T 1991-07-12 1992-07-13 Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos. Expired - Lifetime ES2109983T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72887491A 1991-07-12 1991-07-12

Publications (1)

Publication Number Publication Date
ES2109983T3 true ES2109983T3 (es) 1998-02-01

Family

ID=24928620

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92306396T Expired - Lifetime ES2109983T3 (es) 1991-07-12 1992-07-13 Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.

Country Status (8)

Country Link
EP (1) EP0522884B1 (es)
JP (1) JPH0811070B2 (es)
AT (1) ATE159764T1 (es)
CA (1) CA2073298C (es)
DE (1) DE69222897T2 (es)
DK (1) DK0522884T3 (es)
ES (1) ES2109983T3 (es)
GR (1) GR3025964T3 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
JPH09505475A (ja) * 1993-11-23 1997-06-03 チバ コーニング ダイアグノスティクス コーポレイション 核酸増幅反応における不純物混入の制御プロセスにおけるアンチセンスオリゴマーの使用
JP3517954B2 (ja) * 1994-06-13 2004-04-12 株式会社デンソー 車両のアンチスキッド制御装置
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
EP3690060B1 (en) 2014-10-08 2021-12-08 Cornell University Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
CN113293200B (zh) * 2021-05-28 2022-03-04 北京金匙基因科技有限公司 一种降低或消除二代测序中扩增产物污染的方法及应用
EP4416302A4 (en) * 2021-10-15 2025-04-09 Qiagen Sciences, LLC METHODS FOR PRODUCING DNA LIBRARIES AND THEIR USES
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
EP0415755B1 (en) * 1989-09-01 1995-12-20 Life Technologies Inc. Process for controlling contamination of oligonucleotide-dependent nucleic acid amplification reactions

Also Published As

Publication number Publication date
CA2073298A1 (en) 1993-01-13
DK0522884T3 (da) 1998-07-20
CA2073298C (en) 2007-04-17
ATE159764T1 (de) 1997-11-15
DE69222897T2 (de) 1998-10-01
EP0522884B1 (en) 1997-10-29
GR3025964T3 (en) 1998-04-30
EP0522884A1 (en) 1993-01-13
JPH0690755A (ja) 1994-04-05
DE69222897D1 (de) 1997-12-04
JPH0811070B2 (ja) 1996-02-07

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