ES2114686T3 - Metodos, agentes y kits para la deteccion de compuestos de organofosforo. - Google Patents

Metodos, agentes y kits para la deteccion de compuestos de organofosforo.

Info

Publication number
ES2114686T3
ES2114686T3 ES94915246T ES94915246T ES2114686T3 ES 2114686 T3 ES2114686 T3 ES 2114686T3 ES 94915246 T ES94915246 T ES 94915246T ES 94915246 T ES94915246 T ES 94915246T ES 2114686 T3 ES2114686 T3 ES 2114686T3
Authority
ES
Spain
Prior art keywords
antibodies
protein
methods
methyl
raising
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94915246T
Other languages
English (en)
Inventor
Wendy Alison Matthews
John Nicholas Banks
Mohamed Qasim Chaudhry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minister of Agriculture Fisheries and Food UK
Original Assignee
Minister of Agriculture Fisheries and Food UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minister of Agriculture Fisheries and Food UK filed Critical Minister of Agriculture Fisheries and Food UK
Application granted granted Critical
Publication of ES2114686T3 publication Critical patent/ES2114686T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
    • C07F9/165—Esters of thiophosphoric acids
    • C07F9/1651—Esters of thiophosphoric acids with hydroxyalkyl compounds with further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/576—Six-membered rings
    • C07F9/58—Pyridine rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
    • C07F9/6509—Six-membered rings
    • C07F9/6512—Six-membered rings having the nitrogen atoms in positions 1 and 3
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Luminescent Compositions (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

LA INVENCION SE REFIERE A NUEVOS ANTICUERPOS GENERICOS QUE SON ESPECIFICOS PARA GRUPOS DE ORGANOFOSFATOS, PARTICULARMENTE A UNA CLASE DE PESTICIDAS DE ORGANOFOSFATO DE USO COMUN. TAMBIEN SE SUMINISTRAN COMPUESTOS Y METODOS PARA OBTENER ESTOS ANTICUERPOS, EQUIPOS DE PRUEBA QUE CONTIENE LOS ANTICUERPOS Y METODOS PARA SU USO. PARTICULARMENTE, SE SUMINISTRAN ANTICUERPOS OBJETIVADOS PARA METACRIFOS DE PESTICIDAS DE ORGANOFOSFORO DE BAJO PESO MOLECULAR (POR EJ. POR DEBAJO DE 1000), METILO DE PRIMFOS, FENITROTION, METILO DE CLOPIRIFOS, DICLORVOS Y MALATION. LOS CONJUGADOS PREFERIDOS DE LA INVENCION TIENEN LA FORMULA (II): (RO){SUB, 2}P(S)-Z-[Y]-PROTEINA EN DONDE R ES SEGUN SE DEFINE EN LAS ESPECIFICACIONES Y Z ES O, S O -NH-, Y ES UN GRUPO SEPARADOR Y "PROTEINA" INDICA UNA PROTEINA ADECUADA PARA SU USO EN CONJUGADOS DE HAPTENO-PROTEINA PARA EL PROPOSITO DE ELEVAR LOS ANTICUERPOS. LAS PROTEINAS TIPICAS Y PREFERIDAS SON LA ALBUMINA DEL SUERO BOBINO, LA ALBUMINA DEL HUEVO DE GALLINA, LA HEMOCIANINA DE LA LAPA Y MEZCLAS DE LAS MISMAS.
ES94915246T 1993-05-18 1994-05-18 Metodos, agentes y kits para la deteccion de compuestos de organofosforo. Expired - Lifetime ES2114686T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB939310238A GB9310238D0 (en) 1993-05-18 1993-05-18 Method,agents and kits for detection of organic agents

Publications (1)

Publication Number Publication Date
ES2114686T3 true ES2114686T3 (es) 1998-06-01

Family

ID=10735700

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94915246T Expired - Lifetime ES2114686T3 (es) 1993-05-18 1994-05-18 Metodos, agentes y kits para la deteccion de compuestos de organofosforo.

Country Status (11)

Country Link
US (2) US5830770A (es)
EP (1) EP0699305B1 (es)
JP (1) JPH08510231A (es)
AT (1) ATE165165T1 (es)
AU (1) AU6656694A (es)
DE (1) DE69409643T2 (es)
DK (1) DK0699305T3 (es)
ES (1) ES2114686T3 (es)
GB (2) GB9310238D0 (es)
GR (1) GR3026668T3 (es)
WO (1) WO1994027149A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9310238D0 (en) * 1993-05-18 1993-07-14 Mini Agriculture & Fisheries Method,agents and kits for detection of organic agents
GB9515850D0 (en) * 1995-08-02 1995-10-04 Mini Agriculture & Fisheries Broad specificity antibodies
US6635434B1 (en) 1999-09-17 2003-10-21 Exiqon A/S Immunoassay for pesticides and their degradation products
JP2001275682A (ja) * 2000-03-31 2001-10-09 Kankyo Meneki Gijutsu Kenkyusho:Kk 抗マラチオンモノクローナル抗体をコードする遺伝子
US20020193685A1 (en) * 2001-06-08 2002-12-19 Calypso Medical, Inc. Guided Radiation Therapy System
KR100423526B1 (ko) * 2001-12-28 2004-03-18 이용태 유기인계 포스포로티오에이트 류 농약의 면역분석적검출에 이용되는 합텐의 제조방법
KR100644205B1 (ko) 2005-06-30 2006-11-10 이용태 유기인계 농약의 총체적 검출을 위한 합텐과 항체 및 이를이용한 면역분석방법
JP2011503517A (ja) * 2006-11-13 2011-01-27 アテリス テクノロジーズ，エルエルシー 農薬バイオマーカー
JP2011511930A (ja) * 2007-11-14 2011-04-14 アテリス テクノロジーズ，エルエルシー バイオマーカー検出
CN104262486B (zh) * 2014-09-24 2017-03-15 江苏省农业科学院 杂交瘤细胞株fq‑2g3，其产生的抗甲基毒死蜱单克隆抗体及免疫层析试纸条
CN107064494B (zh) * 2017-04-17 2019-12-24 四川精卫食品检测科技有限公司 一种毒死蜱检测酶联免疫试剂盒
CN110684110A (zh) * 2019-09-20 2020-01-14 北京勤邦生物技术有限公司 一种甲基嘧啶磷单克隆抗体的制备及其应用
CN110981907A (zh) * 2019-11-05 2020-04-10 华南农业大学 一种马拉硫磷半抗原、人工抗原及其制备方法
CN113388037B (zh) * 2021-04-14 2022-04-29 华南农业大学 一种特异性识别杀螟硫磷纳米抗体的制备及其应用
CN114720525B (zh) * 2022-03-22 2024-01-05 华南农业大学 一种对硫磷免疫传感器及其制备方法与应用
EP4543932A1 (en) * 2022-06-21 2025-04-30 Charles M. Thompson Antibody-assisted detoxication of reactive organophosphorus compounds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413915A (en) * 1988-07-12 1995-05-09 Resource Technologies Group, Inc. Method and sensor for detecting toxic chemical exposure effects and metabolic activation of carcinogenic chemical agents
CA2062820C (en) * 1989-06-30 2002-11-12 John H. Skerritt Monoclonal and polyclonal antibodies and test method for determination of fenitrothion and closely related organophosphates
DK0535060T3 (da) * 1990-06-15 1995-07-17 Hoechst Schering Agrevo Gmbh Antifungisk polypeptid samt fremgangsmåde til dets fremstilling
US5283180A (en) * 1990-07-19 1994-02-01 Charm Sciences, Inc. Bioluminescence method for the determination of pesticides
GB9310238D0 (en) * 1993-05-18 1993-07-14 Mini Agriculture & Fisheries Method,agents and kits for detection of organic agents

Also Published As

Publication number Publication date
AU6656694A (en) 1994-12-12
DE69409643D1 (de) 1998-05-20
US5830770A (en) 1998-11-03
US6133049A (en) 2000-10-17
ATE165165T1 (de) 1998-05-15
GB9310238D0 (en) 1993-07-14
DE69409643T2 (de) 1998-08-06
EP0699305A1 (en) 1996-03-06
DK0699305T3 (da) 1998-11-23
GR3026668T3 (en) 1998-07-31
EP0699305B1 (en) 1998-04-15
GB2293384B (en) 1997-05-14
WO1994027149A1 (en) 1994-11-24
GB9522508D0 (en) 1996-01-03
GB2293384A (en) 1996-03-27
JPH08510231A (ja) 1996-10-29

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