ES2117067T3 - Procedimiento para la determinacion inmunoquimica de un analito. - Google Patents

Procedimiento para la determinacion inmunoquimica de un analito.

Info

Publication number
ES2117067T3
ES2117067T3 ES93107931T ES93107931T ES2117067T3 ES 2117067 T3 ES2117067 T3 ES 2117067T3 ES 93107931 T ES93107931 T ES 93107931T ES 93107931 T ES93107931 T ES 93107931T ES 2117067 T3 ES2117067 T3 ES 2117067T3
Authority
ES
Spain
Prior art keywords
union
analyte
procedure
league
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93107931T
Other languages
English (en)
Inventor
Stefan Brust
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Application granted granted Critical
Publication of ES2117067T3 publication Critical patent/ES2117067T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA DETERMINAR INMUNOQUIMICAMENTE UN ANALITO EN UNA MUESTRA MEDIANTE UN PRIMER ASOCIADO DE UNION ESPECIFICO DONDE SE INMOVILIZA AL LIGANDO DE UNION ESPECIFICO EN UN PORTADOR Y SE DETERMINA EL GRADO DE UNION DEL ANALITO A LOS ESPECIFICOS LIGANDOS DE UNION MEDIANTE UN SEGUNDO LIGANDO DE UNION ESPECIFICO QUE PORTA DIRECTAMENTE O INDIRECTAMENTE UNA MARCA. SE CARACTERIZA PORQUE AL PROCESO SE LE AÑADE UN FACTOR DE UNION QUE MUESTRA MAS UN LUGAR DE UNION PARA EL AGENTE QUE SE VA A ANALIZAR, DICHO AGENTE QUE NO ES MARCADO Y NO PRESENTA NINGUNA AFINIDAD CON EL LIGANDO DE UNION ESPECIFICO INMOVILIZADO.
ES93107931T 1992-06-03 1993-05-14 Procedimiento para la determinacion inmunoquimica de un analito. Expired - Lifetime ES2117067T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4218257A DE4218257A1 (de) 1992-06-03 1992-06-03 Verfahren zur immunchemischen Bestimmung eines Analyten

Publications (1)

Publication Number Publication Date
ES2117067T3 true ES2117067T3 (es) 1998-08-01

Family

ID=6460278

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93107931T Expired - Lifetime ES2117067T3 (es) 1992-06-03 1993-05-14 Procedimiento para la determinacion inmunoquimica de un analito.

Country Status (8)

Country Link
US (1) US5914243A (es)
EP (1) EP0572845B1 (es)
JP (1) JP3327488B2 (es)
AT (1) ATE164682T1 (es)
AU (1) AU668937B2 (es)
CA (1) CA2097545C (es)
DE (2) DE4218257A1 (es)
ES (1) ES2117067T3 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4309393A1 (de) * 1993-03-23 1994-09-29 Boehringer Mannheim Gmbh Verringerung des Hook-Effekts in Immuntests mit teilchenförmigem Trägermaterial
DE19629444A1 (de) * 1996-07-22 1998-01-29 Behringwerke Ag Sensitivitätssteigerung bei der immunchemischen Bestimmung eines Analyten
DE59712575D1 (de) * 1996-12-19 2006-04-20 Dade Behring Marburg Gmbh Immundissoziation zur Verbesserung der immunchemischen Bestimmung eines Analyten
EP0957360B1 (de) 1998-05-06 2002-07-31 Roche Diagnostics GmbH Entstörung durch Rheumafaktoren
GB0027064D0 (en) * 2000-11-06 2000-12-20 Randox Lab Ltd Multi-analyte immunoassay
US20040049351A1 (en) * 2002-08-28 2004-03-11 Matson Robert S. Immunosorbent assay in microarray format
DE102004052729A1 (de) 2004-10-30 2006-05-04 Roche Diagnostics Gmbh Immunkomplex-spezifsicher Antikörper zur Reduktion des Nullwerts beim Nachweis von Antigen-spezifisch gebundenen Antikörpern einer bestimmten Immunglobulinklasse in Array-Testformaten
US20080138842A1 (en) * 2006-12-11 2008-06-12 Hans Boehringer Indirect lateral flow sandwich assay
CN102520191B (zh) * 2011-12-19 2014-02-26 苏州大学 一种运用elisa测定蛋白质浓度的方法
CN106324253A (zh) * 2016-08-12 2017-01-11 江苏泽成生物技术有限公司 一种梅毒螺旋体抗体测定试剂盒及其检测方法
US20220229062A1 (en) * 2021-01-21 2022-07-21 Diagnostic Biosystems Method for rapid immunohistochemical detection of an antigen from a biological sample

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4495296A (en) * 1979-05-21 1985-01-22 New York Blood Center, Inc. Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US5011771A (en) * 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4743542A (en) * 1985-04-11 1988-05-10 Ortho Diagnostic Method for forestalling the hook effect in a multi-ligand immunoassay system
DE3834766A1 (de) * 1988-10-12 1990-04-26 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
CA2008100A1 (en) * 1989-01-20 1990-07-20 Hubert Bayer Method for the determination of immunologically detectable substance
AU635355B2 (en) * 1989-11-02 1993-03-18 Teijin Limited Immunoassay of human thrombomodulin, and reagent and kit therefor

Also Published As

Publication number Publication date
AU3996693A (en) 1993-12-09
CA2097545A1 (en) 1993-12-04
CA2097545C (en) 2004-08-17
ATE164682T1 (de) 1998-04-15
JP3327488B2 (ja) 2002-09-24
JPH0658937A (ja) 1994-03-04
DE59308328D1 (de) 1998-05-07
EP0572845B1 (de) 1998-04-01
EP0572845A1 (de) 1993-12-08
AU668937B2 (en) 1996-05-23
DE4218257A1 (de) 1993-12-09
US5914243A (en) 1999-06-22

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