ES2089048T3 - Procedimiento para la deteccion de proteinas que contienen tirosina fosforilada. - Google Patents

Procedimiento para la deteccion de proteinas que contienen tirosina fosforilada.

Info

Publication number
ES2089048T3
ES2089048T3 ES91104912T ES91104912T ES2089048T3 ES 2089048 T3 ES2089048 T3 ES 2089048T3 ES 91104912 T ES91104912 T ES 91104912T ES 91104912 T ES91104912 T ES 91104912T ES 2089048 T3 ES2089048 T3 ES 2089048T3
Authority
ES
Spain
Prior art keywords
thyrosine
phosphorilated
procedure
detection
proteins containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91104912T
Other languages
English (en)
Inventor
Heinz Dr Bodenmuller
Andreas Dr Dessauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2089048T3 publication Critical patent/ES2089048T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5758—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites
    • G01N33/57585—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites involving compounds identifiable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/822—Identified hapten

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

LA INVENCION: SE REFIERE A UN METODO DE COMPROBACION CONTENIENDO TIROSINA QUE CONSISTE EN INCUBAR LA MUESTRA DE UN CUERPO LIQUIDO CON DOS RECEPTORES R ELEVADO 1 Y R (AL CUADRADO) PARA ORIGINAR UNA VARIACION DE SEÑAL Y DETERMINAR LA MODIFICACION DE SEÑAL DE LA MUESTRA CAUSADA POR LA COMBINACION Y SE CARACTERIZA PORQUE UNO DE LOS RECEPTORES CONTIENE UN ANTICUERPO CAPAZ DE COMBINARSE ESPECIFICAMENTE CON LA PROTEINA A COMPROBAR Y EL OTRO RECEPTOR CONTIENE UN ANTICUERPO CAPAZ DE COMBINARSE ESPECIFICAMENTE CON LA TIROSINA FOSFORILADA.
ES91104912T 1990-03-27 1991-03-27 Procedimiento para la deteccion de proteinas que contienen tirosina fosforilada. Expired - Lifetime ES2089048T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4009848A DE4009848A1 (de) 1990-03-27 1990-03-27 Verfahren zum nachweis von phosphoryliertes tyrosin enthaltenden proteinen

Publications (1)

Publication Number Publication Date
ES2089048T3 true ES2089048T3 (es) 1996-10-01

Family

ID=6403171

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91104912T Expired - Lifetime ES2089048T3 (es) 1990-03-27 1991-03-27 Procedimiento para la deteccion de proteinas que contienen tirosina fosforilada.

Country Status (7)

Country Link
US (1) US5580742A (es)
EP (1) EP0449269B1 (es)
JP (1) JPH07104347B2 (es)
AT (1) ATE138205T1 (es)
DE (2) DE4009848A1 (es)
DK (1) DK0449269T3 (es)
ES (1) ES2089048T3 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993003377A1 (en) * 1991-07-31 1993-02-18 Ziltener Hermann J Method for the detection of phosphotyrosine residues
DE69222909T2 (de) * 1991-08-16 1998-03-12 Toshiba Kawasaki Kk Monoklonaler Antikörper gegen ein Synaptophysin
DE4208422A1 (de) * 1992-03-16 1993-09-30 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Mikrometastasen ekto- oder entodermaler Tumoren
US5763198A (en) * 1994-07-22 1998-06-09 Sugen, Inc. Screening assays for compounds
WO1997039326A2 (en) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. In vitro fluorescence polarization assay
US6071707A (en) * 1996-05-07 2000-06-06 Boehringer Ingelheim Pharmaceuticals, Inc. Phosphotyrosine mimics and method for identifying and using same
FR2778744B1 (fr) * 1998-05-14 2000-06-23 Cis Bio Int Dosage immunologique de la chromogranine a humaine (cga) anticorps, reactifs et trousses utilisables pour ce dosage
US6150123A (en) * 1998-09-03 2000-11-21 Centre Integre De Recherches Biocliniques Sur Le Sida (Cirbs) Affinity biotinylation
US6309863B1 (en) * 1999-01-25 2001-10-30 Brookhaven Science Associates Methods for generating phosphorylation site-specific immunological reagents
US7460960B2 (en) * 2002-05-10 2008-12-02 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
US7618788B2 (en) 2002-05-10 2009-11-17 Millipore Corporation Proteome epitope tags and methods of use thereof in protein modification analysis
EP1907837A1 (en) * 2005-06-24 2008-04-09 Beckman Coulter, Inc. Immunoassay of phosphorylated proteins
US7855057B2 (en) * 2006-03-23 2010-12-21 Millipore Corporation Protein splice variant/isoform discrimination and quantitative measurements thereof
WO2007117507A1 (en) * 2006-04-03 2007-10-18 Osi Pharmaceuticals, Inc. A method for the assay of rock kinase activity in cells
US20130052669A1 (en) * 2010-04-30 2013-02-28 Amanda G. Paulovich Compositions and methods for reliably detecting and/or measuring the amount of a modified target protein in a sample
WO2011137388A2 (en) * 2010-04-30 2011-11-03 Fred Hutchinson Cancer Research Center Identification and use of biomarkers for detection and quantification of the level of radiation exposure in a biological sample
US20130084586A1 (en) * 2011-09-30 2013-04-04 The Penn State Research Foundation Rapid, specific and sensitive immunoassays for the detection of highly variable gram negative bacterial antigens

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2057685B (en) * 1979-03-19 1983-12-21 Int Diagnostic Tech Double tagged immunoassay
JPS5745454A (en) * 1980-09-02 1982-03-15 Fuji Photo Film Co Ltd Immunochemical measuring method for various minor components
US4543439A (en) * 1982-12-13 1985-09-24 Massachusetts Institute Of Technology Production and use of monoclonal antibodies to phosphotyrosine-containing proteins
US4758522A (en) * 1985-03-08 1988-07-19 The Regents Of The University Of California Immunoassay for human chromogranin A
JPH01115641A (ja) * 1987-10-27 1989-05-08 Internatl Business Mach Corp <Ibm> サーマル式ドロツプ・オン・デマンド型インクジエツト印刷ヘツド
CH676362A5 (es) * 1987-12-24 1991-01-15 Nippon Shinyaku Co Ltd
DE3804244A1 (de) * 1987-12-24 1989-07-06 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer immunologisch aktiven substanz
EP0353895A1 (en) * 1988-07-18 1990-02-07 MITSUI TOATSU CHEMICALS, Inc. Immunoassay method
DE3829245A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz

Also Published As

Publication number Publication date
DE59107797D1 (de) 1996-06-20
EP0449269A1 (de) 1991-10-02
DK0449269T3 (da) 1996-09-16
ATE138205T1 (de) 1996-06-15
JPH07104347B2 (ja) 1995-11-13
DE4009848A1 (de) 1991-10-02
US5580742A (en) 1996-12-03
JPH04223270A (ja) 1992-08-13
EP0449269B1 (de) 1996-05-15

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