ES2117630T3 - Cosmido bifuncional util para clonar adn actinomicetico. - Google Patents
Cosmido bifuncional util para clonar adn actinomicetico.Info
- Publication number
- ES2117630T3 ES2117630T3 ES91110589T ES91110589T ES2117630T3 ES 2117630 T3 ES2117630 T3 ES 2117630T3 ES 91110589 T ES91110589 T ES 91110589T ES 91110589 T ES91110589 T ES 91110589T ES 2117630 T3 ES2117630 T3 ES 2117630T3
- Authority
- ES
- Spain
- Prior art keywords
- tetracycline
- useful
- actinomytical
- cosmide
- bifunctional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000001588 bifunctional effect Effects 0.000 title 1
- 239000004098 Tetracycline Substances 0.000 abstract 4
- 229960002180 tetracycline Drugs 0.000 abstract 4
- 235000019364 tetracycline Nutrition 0.000 abstract 4
- 229930101283 tetracycline Natural products 0.000 abstract 4
- 150000003522 tetracyclines Chemical class 0.000 abstract 4
- 241000187398 Streptomyces lividans Species 0.000 abstract 3
- 239000004099 Chlortetracycline Substances 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 abstract 2
- 229960004475 chlortetracycline Drugs 0.000 abstract 2
- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 abstract 2
- 235000019365 chlortetracycline Nutrition 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 238000003786 synthesis reaction Methods 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000004128 high performance liquid chromatography Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P29/00—Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Exhaust Gas Treatment By Means Of Catalyst (AREA)
Abstract
ESTA INVENCION SE REFIERE A UNOS NUEVOS Y UTILES COSMIDOS PARA CLONAR Y EXPRESAR EL GRUPO DE GEN DE ADN AISLADO QUE DIRIGE LA SINTESIS DE TETRACICLINA Y CLOROTETRACICLINA, EN UN RECEPTOR HETEOROLOGO TAL COMO STREPTOMYCES LIVIDANS. LOS PLASMIDOS (COSMIDOS) RECLAMADOS SON AISLADOS AL TAMIZAR UN ARCHIVO COSMIDO, CONSTRUIDO EN ESCHERICHIA COLI, PARA LA EXPRESION DE RESISTENCIA TETRACICLINA EN S. LIVIDANS. LOS PLASMIDOS LP (AL CUADRADO) 127 Y LP (AL CUADRADO) 128 SON RECUPERADOS DESDE TALES S. LIVIDANS RESISTENTES A LA TETRACICLINA ASILADOS EXHIBIDOS POR EL ANALISIS DE AGAR HPLC Y FERMENTOS DE CALDO PARA DIRIGIR LA SINTESIS DE TETRACICLINA Y CLOROTETRACICLINA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55804090A | 1990-07-26 | 1990-07-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2117630T3 true ES2117630T3 (es) | 1998-08-16 |
Family
ID=24227940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91110589T Expired - Lifetime ES2117630T3 (es) | 1990-07-26 | 1991-06-26 | Cosmido bifuncional util para clonar adn actinomicetico. |
Country Status (17)
| Country | Link |
|---|---|
| EP (1) | EP0468217B1 (es) |
| JP (1) | JPH0568573A (es) |
| KR (1) | KR100193208B1 (es) |
| AT (1) | ATE166391T1 (es) |
| AU (1) | AU651982B2 (es) |
| CA (1) | CA2047837A1 (es) |
| DE (1) | DE69129429T2 (es) |
| DK (1) | DK0468217T3 (es) |
| ES (1) | ES2117630T3 (es) |
| HU (1) | HU217570B (es) |
| IE (1) | IE912624A1 (es) |
| IL (1) | IL98683A (es) |
| NZ (1) | NZ239043A (es) |
| PT (1) | PT98429B (es) |
| SG (1) | SG43186A1 (es) |
| TW (1) | TW229231B (es) |
| ZA (1) | ZA915866B (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5589385A (en) * | 1990-07-26 | 1996-12-31 | American Cyanamid Company | Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein |
| EP0468220A3 (en) * | 1990-07-26 | 1992-09-23 | American Cyanamid Company | Cloning of the biosynthetic pathway genes for chlortetracycline production from streptomyces aureofaciens & their expression in steptomyces lividans |
| TW318189B (es) * | 1991-12-06 | 1997-10-21 | American Cyanamid Co | |
| US6524812B1 (en) | 1993-10-07 | 2003-02-25 | Regents Of The University Of Minnesota | Genes encoding resistance to DNA alkylating agents |
| US6495348B1 (en) | 1993-10-07 | 2002-12-17 | Regents Of The University Of Minnesota | Mitomycin biosynthetic gene cluster |
| US5989903A (en) * | 1995-06-07 | 1999-11-23 | American Cyanamid Company | Strain for the production of 6-dimethyltetracycline, method for producing the strain and vector for use in the method |
| US6265202B1 (en) | 1998-06-26 | 2001-07-24 | Regents Of The University Of Minnesota | DNA encoding methymycin and pikromycin |
| KR102203404B1 (ko) | 2019-08-14 | 2021-01-15 | 권명오 | 반도체 설비의 배기라인 파우더 제거 장치 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5098837A (en) * | 1988-06-07 | 1992-03-24 | Eli Lilly And Company | Macrolide biosynthetic genes for use in streptomyces and other organisms |
| EP0468220A3 (en) * | 1990-07-26 | 1992-09-23 | American Cyanamid Company | Cloning of the biosynthetic pathway genes for chlortetracycline production from streptomyces aureofaciens & their expression in steptomyces lividans |
-
1991
- 1991-06-26 AT AT91110589T patent/ATE166391T1/de not_active IP Right Cessation
- 1991-06-26 DE DE69129429T patent/DE69129429T2/de not_active Expired - Fee Related
- 1991-06-26 DK DK91110589T patent/DK0468217T3/da active
- 1991-06-26 EP EP91110589A patent/EP0468217B1/en not_active Expired - Lifetime
- 1991-06-26 ES ES91110589T patent/ES2117630T3/es not_active Expired - Lifetime
- 1991-06-27 TW TW080104988A patent/TW229231B/zh not_active IP Right Cessation
- 1991-07-01 IL IL9868391A patent/IL98683A/en not_active IP Right Cessation
- 1991-07-19 NZ NZ239043A patent/NZ239043A/xx unknown
- 1991-07-24 JP JP3206089A patent/JPH0568573A/ja active Pending
- 1991-07-24 CA CA002047837A patent/CA2047837A1/en not_active Abandoned
- 1991-07-24 PT PT98429A patent/PT98429B/pt not_active IP Right Cessation
- 1991-07-25 IE IE262491A patent/IE912624A1/en not_active IP Right Cessation
- 1991-07-25 AU AU81339/91A patent/AU651982B2/en not_active Ceased
- 1991-07-25 ZA ZA915866A patent/ZA915866B/xx unknown
- 1991-07-26 KR KR1019910013059A patent/KR100193208B1/ko not_active Expired - Fee Related
- 1991-07-26 HU HU515/91A patent/HU217570B/hu not_active IP Right Cessation
-
1996
- 1996-08-27 SG SG1996005117A patent/SG43186A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT98429B (pt) | 1999-01-29 |
| EP0468217B1 (en) | 1998-05-20 |
| IL98683A0 (en) | 1992-07-15 |
| PT98429A (pt) | 1992-05-29 |
| IE912624A1 (en) | 1992-01-29 |
| NZ239043A (en) | 1993-09-27 |
| KR100193208B1 (ko) | 1999-06-15 |
| CA2047837A1 (en) | 1992-01-27 |
| DE69129429D1 (de) | 1998-06-25 |
| DK0468217T3 (da) | 1998-10-07 |
| HU217570B (hu) | 2000-02-28 |
| ATE166391T1 (de) | 1998-06-15 |
| TW229231B (es) | 1994-09-01 |
| IL98683A (en) | 2002-11-10 |
| DE69129429T2 (de) | 1998-09-24 |
| AU8133991A (en) | 1992-01-30 |
| HU912515D0 (en) | 1992-01-28 |
| ZA915866B (en) | 1992-04-29 |
| JPH0568573A (ja) | 1993-03-23 |
| HUT60776A (en) | 1992-10-28 |
| SG43186A1 (en) | 1997-10-17 |
| EP0468217A2 (en) | 1992-01-29 |
| EP0468217A3 (en) | 1992-09-30 |
| AU651982B2 (en) | 1994-08-11 |
| KR920002777A (ko) | 1992-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PT84801A (en) | Secretory signal selection vectors for extracellular protein synthesis in bacilli | |
| ES2117630T3 (es) | Cosmido bifuncional util para clonar adn actinomicetico. | |
| DK0598029T3 (da) | Retrovirale vektorer indeholdende interne ribosome indgangssteder | |
| HU900226D0 (en) | Process for production of expression vectors expressing amidation enzymes | |
| DK0792363T3 (da) | Nye pesticide proteiner og stammer | |
| DE68923728D1 (de) | Insektenresistente Salatpflanzen. | |
| PT2169066E (pt) | Ácido nucleico promotor derivado de bactérias do género corynebacterium, cassete de expressão compreendendo o promotor e vector compreendendo a cassete, célula hospedeira compreendendo o vector e método de expressão de um gene utilizando a célula | |
| CA2197160A1 (en) | Platenolide synthase gene | |
| CA2047833A1 (en) | Cloning of the biosynthetic pathway genes for chlortetracycline production from streptomyces aureofaciens and their expression in steptomyces lividans | |
| ATE256188T1 (de) | Verbesserte riboflavin-herstellung | |
| GR3030722T3 (en) | Improved synthesis of bisindolylmaleimides. | |
| EP0226979A3 (en) | Process for producing the protein of an aequorin activity by using a photoprotein aequorin gene | |
| AU7066187A (en) | Enhanced protein production in bacteria by employing a novel ribosome binding site | |
| AU574857B2 (en) | 4,6-dialkoxy pyridine-3(5)-(di) carboxylate compounds | |
| CZ20191998A3 (cs) | Způsob selektivní ochrany derivátů baccatinu a jeho použití při syntéze taxanů | |
| FR2661188B1 (fr) | Nouveaux vecteurs de clonage et/ou d'expression, procede de preparation et leur utilisation. | |
| DE3879440D1 (de) | Dactylocyclin a und dactylocyclin b. | |
| AU652099C (en) | Alkene intermediates for the production of certain 1,2-dioxetane compounds | |
| ES2001992A6 (es) | Procedimiento para la construccion de dos nuevos plasmidos prmel y prmels utiles para la expresion genetica en bacterias escherichia coli de proteinas de fusion con los primeros catorce aminoacidos de la proteina p4 del bacteriofago &29 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 468217 Country of ref document: ES |