ES2118049B1 - Carbamato quinasa: su preparacion y uso. - Google Patents

Carbamato quinasa: su preparacion y uso.

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Publication number
ES2118049B1
ES2118049B1 ES9602525A ES9602525A ES2118049B1 ES 2118049 B1 ES2118049 B1 ES 2118049B1 ES 9602525 A ES9602525 A ES 9602525A ES 9602525 A ES9602525 A ES 9602525A ES 2118049 B1 ES2118049 B1 ES 2118049B1
Authority
ES
Spain
Prior art keywords
carbamate
pix
kinase
preparation
adp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES9602525A
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English (en)
Other versions
ES2118049A1 (es
Inventor
Zamora Vicente Rubio
Moreno Alberto Marina
Andres Belen Barcelona
Porcar Enrique Pertegaz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Científicas
Original Assignee
Consejo Superior de Investigaciones Científicas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Científicas filed Critical Consejo Superior de Investigaciones Científicas
Priority to ES9602525A priority Critical patent/ES2118049B1/es
Publication of ES2118049A1 publication Critical patent/ES2118049A1/es
Application granted granted Critical
Publication of ES2118049B1 publication Critical patent/ES2118049B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

CARBAMATO QUINASA: SU PREPARACION Y USO. MEDIANTE TECNICAS DE INGENIERIA GENETICA SE PREPARA LA CARBAMATO QUINASA DE ENTEROCOCCUS<PIX> (ANTES STREPTOCOCCUS<PIX>) FAECALIS<PIX> QUE CATALIZA EN PRESENCIA DE MG2+ LA FORMACION DE CARBAMATO Y TRIFOSFATO DE ADENOSINA (ATP), A PARTIR DE CARBAMIL FOSFATO Y DIFOSFATO DE ADENOSINA (ADP), ASI COMO LA REACCION INVERSA, DESDE CARBAMATO Y ATP A CARBAMIL FOSFATO Y ADP. SE AISLA UN GEN QUE CODIFICA PARA CARBAMATO QUINASA DE LA FUENTE USUAL DEL ENZIMA ( ENTEROCOCCUS FAECALIS<PIX>), SE SECUENCIA Y SE USA PARA TRANSFORMAR UN MICROORGANISMO Y GENERAR UN TRANSFORMANTE QUE PUEDE CULTIVARSE PARA PRODUCIR QUINASA CON GRAN RENDIMIENTO. SE UTILIZA UN PROCEDIMIENTO DE PURIFICACION QUE PERMITE GENERAR ENZIMA ESENCIALMENTE PURA.
ES9602525A 1996-11-29 1996-11-29 Carbamato quinasa: su preparacion y uso. Expired - Lifetime ES2118049B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9602525A ES2118049B1 (es) 1996-11-29 1996-11-29 Carbamato quinasa: su preparacion y uso.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9602525A ES2118049B1 (es) 1996-11-29 1996-11-29 Carbamato quinasa: su preparacion y uso.

Publications (2)

Publication Number Publication Date
ES2118049A1 ES2118049A1 (es) 1998-09-01
ES2118049B1 true ES2118049B1 (es) 1999-04-01

Family

ID=8296856

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9602525A Expired - Lifetime ES2118049B1 (es) 1996-11-29 1996-11-29 Carbamato quinasa: su preparacion y uso.

Country Status (1)

Country Link
ES (1) ES2118049B1 (es)

Also Published As

Publication number Publication date
ES2118049A1 (es) 1998-09-01

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