ES2123542T3 - Preparaciones pcr in situ, y su uso. - Google Patents

Preparaciones pcr in situ, y su uso.

Info

Publication number
ES2123542T3
ES2123542T3 ES92306701T ES92306701T ES2123542T3 ES 2123542 T3 ES2123542 T3 ES 2123542T3 ES 92306701 T ES92306701 T ES 92306701T ES 92306701 T ES92306701 T ES 92306701T ES 2123542 T3 ES2123542 T3 ES 2123542T3
Authority
ES
Spain
Prior art keywords
pcr
degrees
thermal cycle
reaction
beginning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92306701T
Other languages
English (en)
Inventor
Will Bloch
Gerard J Nuovo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Research Foundation of the State University of New York
Original Assignee
F Hoffmann La Roche AG
Research Foundation of the State University of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Research Foundation of the State University of New York filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2123542T3 publication Critical patent/ES2123542T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LAS MEJORAS EN LA REACCION EN CADENA DE LA POLIMERASA IN SITU (PCR), UN PROCESO DE AMPLIFICACION ENZIMATICA IN VITRO DE SECUENCIAS DE ACIDO NUCLEICO ESPECIFICAS DENTRO DE LAS CELULAS DONDE SE ORIGINAN, SE PUEDEN ALCANZAR A BASE DE CAMBIAR EL MODO EN QUE SE INICIA LA REACCION EN CADENA. EL INICIO DE LA REACCION SE RETRASA HASTA EL PRINCIPIO DEL CICLAJE TERMICO DE LA PCR, BIEN A BASE DE RETENER A UN SUBGRUPO DE REACTIVOS DE LA PCR DE LA PREPARACION CELULAR HASTA QUE LA PREPARACION SE HAYA CALENTADO A UNA TEMPERATURA ENTRE 50 (GRADOS) Y 80 (GRADOS) C, INMEDIATAMENTE ANTES DE QUE COMIENCE EL CICLAJE TERMICO, O A BASE DE AÑADIR A LOS REACTIVOS DE LA PCR UNA PROTEINA AGLUTINANTE DE ADN DE UNA SOLA FIBRA QUE BLOQUEA LA REACCION A UNA TEMPERATURA INFERIOR A LOS 50 (GRADOS) C. SI LA PCR IN SITU SE LLEVA A CABO EN PREPARACIONES CELULARES YA UNIDAS A PORTAOBJETOS DE MICROSCOPIO, EL CICLAJE TERMICO ES MAS FACIL MEDIANTE EL USO DE UN BLOQUE DE MUESTRA DE CICLO TERMICO O UN COMPARTIMIENTO DISEÑADO DE MANERA OPTIMA PARA SUJETAR EL PORTAOBJETOS DE MICROSCOPIO Y CUALQUIER BARRERA DE VAPOR QUE CUBRE AL PORTAOBJETOS.
ES92306701T 1991-07-23 1992-07-22 Preparaciones pcr in situ, y su uso. Expired - Lifetime ES2123542T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US73341991A 1991-07-23 1991-07-23

Publications (1)

Publication Number Publication Date
ES2123542T3 true ES2123542T3 (es) 1999-01-16

Family

ID=24947512

Family Applications (2)

Application Number Title Priority Date Filing Date
ES92306701T Expired - Lifetime ES2123542T3 (es) 1991-07-23 1992-07-22 Preparaciones pcr in situ, y su uso.
ES98200769T Expired - Lifetime ES2165653T3 (es) 1991-07-23 1992-07-22 Equipo ciclador termico.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES98200769T Expired - Lifetime ES2165653T3 (es) 1991-07-23 1992-07-22 Equipo ciclador termico.

Country Status (8)

Country Link
US (1) US5538871A (es)
EP (2) EP0863213B1 (es)
JP (1) JPH0873B2 (es)
AU (1) AU645915B2 (es)
CA (2) CA2218875C (es)
DE (2) DE69227076T2 (es)
DK (2) DK0524808T3 (es)
ES (2) ES2123542T3 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2169701A1 (es) * 2000-12-21 2002-07-01 Univ Jaen Reactor microcristalografico.

Families Citing this family (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1994010564A1 (de) * 1992-11-05 1994-05-11 Evotec Biosystems Gmbh Verfahren zur trennung von substanzen aus verdünnten lösungen oder suspensionen
US5364790A (en) * 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
AU7640494A (en) * 1993-09-03 1995-03-22 Cellpro, Incorporated Methods for quantifying the number of cells containing a selected nucleic acid sequence in a heterogenous population of cells
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
DE4409436A1 (de) * 1994-03-19 1995-09-21 Boehringer Mannheim Gmbh Verfahren zur Bearbeitung von Nukleinsäuren
GB9507232D0 (en) * 1995-04-07 1995-05-31 Gatsby Charitable Foundation Plant pathogen resistance genes and uses thereof
JPH08196299A (ja) * 1995-01-26 1996-08-06 Tosoh Corp サーマルサイクリング反応装置及びこれに用いる反応容器
EP0826066B1 (en) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
EP1291441A3 (en) * 1995-09-12 2003-03-19 Becton, Dickinson and Company Device and method for DNA amplification and assay
WO1997013869A1 (en) * 1995-10-10 1997-04-17 The Regents Of The University Of California Detection of allele-specific mutations by allele-specific in situ reverse transcriptase polymerase chain reaction
CA2231729A1 (en) * 1995-11-14 1997-05-22 William L. Ragland Efficient method of detecting an infectious agent in blood
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AUPN923596A0 (en) * 1996-04-12 1996-05-09 Australian Biomedical Corporation Limited Method and apparatus for treatment of human or animal cell samples
DE19627290A1 (de) * 1996-07-06 1998-01-08 Boehringer Mannheim Gmbh Bestimmung von Analyten unter Verwendung zweier Markierungen
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9624165D0 (en) * 1996-11-19 1997-01-08 Amdex A S Use of nucleic acids bound to carrier macromolecules
US6703247B1 (en) 1996-12-23 2004-03-09 American Registry Of Pathology Apparatus and methods for efficient processing of biological samples on slides
US6074868A (en) * 1997-03-03 2000-06-13 Regents Of The University Of Minnesota Alumina plate method and device for controlling temperature
FR2769323B1 (fr) * 1997-10-08 2001-07-13 Suez Lyonnaise Des Eaux Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon
DE59804008D1 (de) * 1997-12-05 2002-06-06 Max Planck Gesellschaft Verfahren zur identifikation von nucleinsäuren durch matrix-assistierte laser desorptions/ionisations massenspektrometrie
US7396508B1 (en) 2000-07-12 2008-07-08 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) * 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
ES2354598T3 (es) * 1998-02-27 2011-03-16 Ventana Medical Systems, Inc. Aparato de patología molecular automatizado que tiene calefactores de portaobjetos independientes.
US7569344B2 (en) * 1998-10-26 2009-08-04 Ventana Medical Systems, Inc. Detection of human papilloma virus in papanicolaou (Pap) smears
WO2000038838A1 (en) * 1998-12-23 2000-07-06 American Registry Of Pathology Apparatus and methods for efficient processing of biological samples on slides
US20060216744A1 (en) * 1998-12-23 2006-09-28 American Registry Of Pathology Armed Forces Institute Of Pathology Apparatus and methods for efficient processing of biological samples on slides
CA2365263A1 (en) * 1999-03-12 2000-09-21 Mcgill University A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
DE19923584C2 (de) * 1999-05-21 2002-01-24 Memorec Medical Molecular Res Inkubationssystem
US6187567B1 (en) 1999-09-01 2001-02-13 Akzo Nobel N.V. Methods and reagents for in situ amplification
US20060275784A1 (en) * 1999-10-26 2006-12-07 Ventana Medical Systems, Inc. Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears
US6300124B1 (en) 1999-11-02 2001-10-09 Regents Of The University Of Minnesota Device and method to directly control the temperature of microscope slides
WO2001042507A1 (en) * 1999-12-09 2001-06-14 Advanced Research & Technology Institute Fluorescent in situ rt-pcr
US6448014B2 (en) * 2000-04-19 2002-09-10 Research Development Foundation PCR-hybridization assays specific for integrated retroviruses
US6864056B1 (en) * 2000-07-31 2005-03-08 The United States Of America As Represented By The Secretary Of The Army Method for detecting ribosome inactivating proteins
US6783943B2 (en) * 2000-12-20 2004-08-31 The Regents Of The University Of California Rolling circle amplification detection of RNA and DNA
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
WO2003074740A1 (en) * 2002-03-01 2003-09-12 Ravgen, Inc. Rapid analysis of variations in a genome
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
WO2004022770A2 (en) * 2002-09-05 2004-03-18 Invitrogen Corporation Compositions and methods for synthesizing nucleic acids
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20050153303A1 (en) * 2003-02-12 2005-07-14 Agroterra Biotech Inc. Method of mutagenic chain reaction
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
US20050032464A1 (en) * 2003-08-07 2005-02-10 Swisher Robert G. Polishing pad having edge surface treatment
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
US20070122909A1 (en) * 2003-10-20 2007-05-31 Syssmex Corporation Method of treating cells
US7759057B2 (en) * 2004-02-04 2010-07-20 Ut-Battelle, Llc Method for analyzing microbial communities
EP1769247A1 (en) * 2004-02-13 2007-04-04 Molecular Probes, Inc. Biotin recognition sensors and high-throughput assays
GB0406015D0 (en) * 2004-03-17 2004-04-21 Dynal Biotech Asa Improvements in magnetic polymer particles
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US20060189893A1 (en) * 2005-01-06 2006-08-24 Diamics, Inc. Systems and methods for detecting abnormal cells
US20060161076A1 (en) * 2005-01-06 2006-07-20 Diamics, Inc. Systems and methods for collection of cell clusters
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20060263811A1 (en) * 2005-05-03 2006-11-23 Geunsook Jeon Materials and kits for use in hot-start PCR, and methods of amplifying nucleic acids in a polymerase chain reaction
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
NZ571459A (en) 2006-03-29 2011-09-30 Merial Ltd Vaccine against streptococcus equi
WO2007149350A2 (en) * 2006-06-19 2007-12-27 Becton, Dickinson And Company Methods and compositions for obtaining amplifiable nucleic acids from tissues, cells, or viruses exposed to transport media
WO2008038507A1 (en) * 2006-09-26 2008-04-03 Olympus Corporation Substratum and method of reactive liquid reaction
JP5684563B2 (ja) 2007-04-06 2015-03-11 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ カルバペネマーゼ遺伝子の同定のための組成物および方法
EP2245199B1 (en) * 2008-02-01 2013-11-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
EP2285965A1 (en) * 2008-03-31 2011-02-23 Pfenex Inc A design for rapidly cloning one or more polypeptide chains into an expression system
IT1397110B1 (it) * 2008-12-29 2012-12-28 St Microelectronics Rousset Microreattore autosigillante e metodo per eseguire una reazione
WO2010096723A1 (en) 2009-02-19 2010-08-26 Geneohm Sciences, Inc. Methods for the detection and identification of extended spectrum beta lactamases
BR112012001077A2 (pt) 2009-07-16 2020-08-11 The General Hospital Corporation extração de ácido nucleico, métodos para avaliar a qualidade de uma extração de ácido nucleico e de uma amostra biológica, para ober ácido nucleico a partir de uma amostra biológica, para analisar rna de microvesículas e para diagnosticar e monitorar um indivíduo, e, kit
BR112012004699B1 (pt) 2009-09-03 2019-10-15 Becton, Dickinson And Company Métodos, composições e kits para lise química direta
EP2475989A4 (en) 2009-09-09 2013-02-27 Gen Hospital Corp USE OF MICROVESICLES IN THE ANALYSIS OF KRAS MUTATIONS
WO2011031877A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
US8383793B2 (en) 2010-04-15 2013-02-26 St. Jude Children's Research Hospital Methods and compositions for the diagnosis and treatment of cancer resistant to anaplastic lymphoma kinase (ALK) kinase inhibitors
US20140045915A1 (en) 2010-08-31 2014-02-13 The General Hospital Corporation Cancer-related biological materials in microvesicles
WO2012032519A2 (en) 2010-09-07 2012-03-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of diagnosing parkinson's disease
JP6219721B2 (ja) 2010-11-10 2017-10-25 エキソソーム ダイアグノスティックス インコーポレイテッド 核酸含有粒子の単離および該粒子からの核酸の抽出のための方法
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US20120183960A1 (en) 2010-12-07 2012-07-19 Enterologics, Inc. Method for identifying e. coli m-17
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
EP2481812A1 (en) 2011-01-31 2012-08-01 Westfälische Wilhelms-Universität Münster Molecular sexing of avian subjects
WO2012142233A1 (en) 2011-04-14 2012-10-18 St. Jude Children's Research Hospital Methods and compositions for detecting and modulating a novel mtor complex
CA2835641C (en) 2011-05-11 2019-05-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
EP3492606B1 (en) 2011-08-22 2023-10-04 Exosome Diagnostics, Inc. Urine biomarkers
EP2776587A4 (en) 2011-11-10 2015-07-15 Exosome Diagnostics Inc LIQUOR ASSAY
DK2809810T3 (da) 2012-01-06 2020-02-03 Viomics Inc System og fremgangsmåde til påvisning i perifert blod af rna'er der er forandret af lungecancer
CN104284725B (zh) * 2012-02-27 2017-04-26 洛桑联邦理工学院 具有可拆卸载玻片的样品处理装置
RU2716210C2 (ru) 2012-04-06 2020-03-06 Дженеом Сайенсиз Канада, Инк. Последовательности для обнаружения и распознавания устойчивого к метициллину золотистого стафилококка (staphylococcus aureus) (mrsa) с mrej типа xxi
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
CN104995311A (zh) 2012-08-30 2015-10-21 外来体诊断公司 用于核酸测定的对照
CA2887058C (en) 2012-10-03 2022-02-22 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
ES2859645T3 (es) 2013-03-14 2021-10-04 Mayo Found Medical Education & Res Detección de neoplasia
AU2014305994B2 (en) 2013-08-06 2019-02-21 Exosome Diagnostics, Inc. Urine biomarker cohorts, gene expression signatures, and methods of use thereof
US10844436B2 (en) 2014-04-01 2020-11-24 Cornell University Use of double-stranded DNA in exosomes: a novel biomarker in cancer detection
EP3132055A2 (en) 2014-04-14 2017-02-22 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
CN104789568B (zh) * 2015-03-20 2017-11-21 中国科学院南海海洋研究所 一种用于检测石斑鱼虹彩病毒感染的dna核酸适配体及其筛选方法和应用
CN104789569B (zh) * 2015-03-20 2017-11-21 中国科学院南海海洋研究所 一种用于检测石斑鱼虹彩病毒感染的dna核酸适配体及其筛选方法和应用
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
CN108138235B (zh) 2015-08-31 2022-04-15 梅约医药教育及研究基金会 检测胃肿瘤
WO2017062989A1 (en) 2015-10-08 2017-04-13 Urology Diagnostics, Inc. Diagnostic assay for urine monitoring of bladder cancer
AU2016343937B2 (en) 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
IL287757B (en) 2016-03-03 2022-07-01 Memed Diagnostics Ltd Analyzing rna for diagnosing infection type
SG11201809005TA (en) 2016-04-15 2018-11-29 Exosome Diagnostics Inc Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer
AU2017263886B2 (en) 2016-05-13 2023-11-09 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
EP4656715A3 (en) 2016-07-29 2026-02-25 Streck LLC Suspension composition for hematology analysis control
US11396676B2 (en) 2016-10-21 2022-07-26 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
JP7280823B2 (ja) 2016-11-30 2023-05-24 エクソサム ダイアグノスティクス，インコーポレイティド 非小細胞肺癌患者由来のエクソソームｒｎａ及び無細胞ｄｎａを使用して血漿中の変異を検出するための方法と組成物
US20200149036A1 (en) 2017-01-02 2020-05-14 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
KR102892245B1 (ko) 2017-01-27 2025-11-27 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 결장 신조직형성의 검출
US11899024B2 (en) 2017-07-12 2024-02-13 Exosome Diagnostics, Inc. Treatment and diagnosis of parkinson's disease using isolated and enriched populations of biofluid-derived extracellular vesicles
WO2019018537A1 (en) 2017-07-18 2019-01-24 Exosome Diagnostics, Inc. NUCLEIC ACID SEQUENCING ASSOCIATED WITH EXOSOMAL ISOLATION IN PATIENTS WITH MULTIPLE GLIOBLASTOMA
CN115119829B (zh) 2017-10-19 2024-07-12 斯特雷克股份有限公司 用于胞外囊泡的溶血和凝血调节以及稳定化的组合物
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
EP3768858A1 (en) 2018-03-19 2021-01-27 Modernatx, Inc. Assembly and error reduction of synthetic genes from oligonucleotides
WO2019236853A1 (en) 2018-06-06 2019-12-12 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
WO2020060994A1 (en) 2018-09-17 2020-03-26 The University Of North Carolina At Chapel Hill Method for quantifying dna fragments in a sample by size
WO2020106853A1 (en) 2018-11-20 2020-05-28 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
WO2021076770A1 (en) * 2019-10-18 2021-04-22 The Board Of Trustees Of The Leland Stanford Junior University Clinical- and industrial-scale intact-tissue sequencing
EP3901286A1 (en) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
EP4055189B1 (en) 2019-11-04 2025-01-01 Mirnax Biosens, S.L. Bivalent reverse primer
EP4072673A4 (en) 2019-12-09 2024-02-21 Beth Israel Deaconess Medical Center, Inc. COMPOSITIONS AND METHODS FOR IDENTIFYING AND MODULATING THROMBOSIS IN A CANCER PATIENT
US11254989B2 (en) 2020-03-16 2022-02-22 The University Of North Carolina At Chapel Hill Compositions and methods for the selective detection of tumor-derived viral DNA
WO2021204642A1 (en) 2020-04-06 2021-10-14 Fast Track Diagnostics Luxembourg S.À.R.L. Pcr based diagnostic kit, compositions and methods for amplification and detection of sars-cov-2
IL278473A (en) 2020-11-03 2022-06-01 Yeda Res & Dev Methods for diagnosing and determining treatment in multiple myeloma
FR3130844B1 (fr) 2021-12-22 2026-04-24 Bforcure Composition et procédé d’amplification de séquences d’acides nucléiques
EP4573217A1 (en) 2022-08-18 2025-06-25 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. A method for determining the tissue or cell of origin of dna
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
WO2025203031A2 (en) 2024-03-26 2025-10-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of cancer detection by discordant methylation in cfdna

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1339653C (en) * 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5021335A (en) * 1988-06-17 1991-06-04 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5281516A (en) * 1988-08-02 1994-01-25 Gene Tec Corporation Temperature control apparatus and method
WO1991006679A1 (en) * 1989-10-24 1991-05-16 Stratagene An improved method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5346672A (en) * 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
US5230997A (en) * 1990-07-19 1993-07-27 The United States Of America As Represented By The Department Of Health And Human Services Methods of detecting the presence of human herpesvirus-7 infection
GB9123463D0 (en) * 1991-11-05 1991-12-18 Hybaid Ltd Reaction temperature control device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2169701A1 (es) * 2000-12-21 2002-07-01 Univ Jaen Reactor microcristalografico.

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EP0524808A2 (en) 1993-01-27
US5538871A (en) 1996-07-23
DK0524808T3 (da) 1999-06-14
CA2218875C (en) 2000-11-07
DK0863213T3 (da) 2002-01-21
AU2041992A (en) 1993-02-25
AU645915B2 (en) 1994-01-27
CA2074214A1 (en) 1993-01-24
EP0524808B1 (en) 1998-09-23
ES2165653T3 (es) 2002-03-16
JPH05336971A (ja) 1993-12-21
EP0863213B1 (en) 2001-10-31
CA2074214C (en) 2000-02-22
JPH0873B2 (ja) 1996-01-10
CA2218875A1 (en) 1993-01-24
DE69232181T2 (de) 2002-06-20
DE69227076D1 (de) 1998-10-29
EP0524808A3 (en) 1993-07-14
DE69232181D1 (de) 2001-12-06
EP0863213A1 (en) 1998-09-09

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