ES2128372T3 - Un metodo quimioluminiscente para la cuantificacion de adn humano. - Google Patents

Un metodo quimioluminiscente para la cuantificacion de adn humano.

Info

Publication number
ES2128372T3
ES2128372T3 ES93113261T ES93113261T ES2128372T3 ES 2128372 T3 ES2128372 T3 ES 2128372T3 ES 93113261 T ES93113261 T ES 93113261T ES 93113261 T ES93113261 T ES 93113261T ES 2128372 T3 ES2128372 T3 ES 2128372T3
Authority
ES
Spain
Prior art keywords
methods
sample
chemioluminiscent
human dna
quantification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93113261T
Other languages
English (en)
Inventor
Rebecca L Reynolds
P Sean Walsh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2128372T3 publication Critical patent/ES2128372T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A METODOS Y REACTIVOS PARA LA ESTIMACION DE LA CANTIDAD DE ADN HUMANO CONTENIDO EN UNA MUESTRA.EL ADN DE MUESTRA INMOVILIZADO SE HIBRIDIZA EN UNA SONDA OLIGONUCLEOTIDA BIOREDUCIDA QUE HIBRIDIZA UNA SECUENCIA DE ADN DE MITOCONDRIA O GENOMICO HUMANO. EL ENLACE SUBSIGUIENTE DE PEROXIDASA DE ESTREPTAVIDINHORSEADISH A LA SONDA ENLAZADA PERMITE LA DETECCION QUIMIOLUMINISCENTE UTILIZANDO UNA PELICULA DE RAYOS X Y REACTIVO BASADO EN LUMINOL. ADEMAS, LA PRESENTE INVENCION PRESENTA METODOS Y REACTIVOS PARA EVALUAR LA CALIDAD DE ADN CONTENIDO EN UNA MUESTRA. LA MUESTRA ES PRIMERAMENTE FRACCIONADA A UN TAMAÑO POR ELECTROFERESIS DE GEL DE AGAROSA, Y DESPUES SE INMOVILIZA, HIBRIDIZA A SONDA OLIGONUCLEOTIDA BIOREDUCIDA, Y SE DETECTA UTILIZANDO LOS METODOS DE QUIMIOLUMINISCENCIA COMO SU USO EN METODOS DE ESTIMACION DE CANTIDAD DE LA PRESENTE INVENCION.
ES93113261T 1992-08-28 1993-08-19 Un metodo quimioluminiscente para la cuantificacion de adn humano. Expired - Lifetime ES2128372T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/938,084 US5464945A (en) 1992-08-28 1992-08-28 Oligonucleotide probes specific for the human alpha satellite locus

Publications (1)

Publication Number Publication Date
ES2128372T3 true ES2128372T3 (es) 1999-05-16

Family

ID=25470852

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93113261T Expired - Lifetime ES2128372T3 (es) 1992-08-28 1993-08-19 Un metodo quimioluminiscente para la cuantificacion de adn humano.

Country Status (8)

Country Link
US (1) US5464945A (es)
EP (1) EP0594959B1 (es)
JP (1) JP3759629B2 (es)
CA (1) CA2104767C (es)
DE (1) DE69323032T2 (es)
DK (1) DK0594959T3 (es)
ES (1) ES2128372T3 (es)
GR (1) GR3029875T3 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455689B1 (en) * 1993-04-30 2002-09-24 Biognostik Gesellschaft für Biomolekulare Diagnostik mbH Antisense-oligonucleotides for transforming growth factor-β (TGF-β)
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
DE69637086T2 (de) * 1995-10-12 2008-01-10 Lansdorp, Peter M., Vancouver Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül
US5733732A (en) * 1996-01-03 1998-03-31 University Of Iowa Research Foundation Methods for detecting primary adhalinopathy
US5754524A (en) * 1996-08-30 1998-05-19 Wark; Barry J. Computerized method and system for analysis of an electrophoresis gel test
EP0848059A1 (en) * 1996-12-12 1998-06-17 Vetigen Mammalian ICYP (Iodocyanopindolol) receptor and its applications
US6489095B2 (en) 1998-06-15 2002-12-03 Mitokor Diagnostic method based on quantification of extramitochondrial DNA
US6441149B1 (en) 1998-06-15 2002-08-27 Mitokor Diagnostic method based on quantification of extramitochondrial DNA
US6153389A (en) * 1999-02-22 2000-11-28 Haarer; Brian K. DNA additives as a mechanism for unambiguously marking biological samples
KR20000074881A (ko) * 1999-05-26 2000-12-15 황승용 마이크로웰을 이용한 디엔에이 돌연변이의 확인방법 및 키트
US6759196B2 (en) 2000-02-23 2004-07-06 Childrens Hospital Los Angeles Method of detecting mitochondrial dysfunction
GB0016813D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
KR20000072201A (ko) * 2000-08-17 2000-12-05 김희태 여러 종류의 디엔에이 프로브들을 사용한 취약-엑스증후군의 진단 방법
WO2002068689A1 (en) * 2001-02-24 2002-09-06 Childrens Hospital Los Angeles Method of detecting mitochondrial dysfunction
KR20020041344A (ko) * 2002-02-26 2002-06-01 김희태 멤브레인 크로마토그래프 기법을 활용하여핵산중합반응으로 증폭된 핵산을 검출하는 방법
US6946285B2 (en) * 2002-04-29 2005-09-20 Agilent Technologies, Inc. Arrays with elongated features
KR100492393B1 (ko) * 2002-12-02 2005-05-30 굿젠 주식회사 멤브레인 크로마토그래프 기법에 의한 핵산 검출 방법 및이를 위한 킷트
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
JP2012529908A (ja) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
US10519502B2 (en) 2013-10-31 2019-12-31 The Children's Hospital Of Philadelphia Mitochondrial disease genetic diagnostics
US9758786B2 (en) 2016-02-09 2017-09-12 Autotelic, Llc Compositions and methods for treating pancreatic cancer
WO2018093777A1 (en) * 2016-11-15 2018-05-24 The Regents Of The University Of Michigan Centromere analysis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA2032331A1 (en) * 1990-01-22 1991-07-23 Annie L. Wu Method and kits for detecting human leukocyte antigen dna

Also Published As

Publication number Publication date
EP0594959A1 (en) 1994-05-04
GR3029875T3 (en) 1999-07-30
US5464945A (en) 1995-11-07
DE69323032D1 (de) 1999-02-25
CA2104767A1 (en) 1994-03-01
DK0594959T3 (da) 1999-09-06
JPH06189798A (ja) 1994-07-12
JP3759629B2 (ja) 2006-03-29
CA2104767C (en) 2009-06-23
EP0594959B1 (en) 1999-01-13
DE69323032T2 (de) 1999-08-26

Similar Documents

Publication Publication Date Title
ES2128372T3 (es) Un metodo quimioluminiscente para la cuantificacion de adn humano.
DE50000288D1 (de) Vorrichtung und verfahren zum verbergen von informationen und vorrichtung und verfahren zum extrahieren von informationen
ES2125272T3 (es) Metodo de biosensor de sonda genica.
ATE338951T1 (de) Vorrichtung zur automatischen durchführung von laboratoriumprüfungen
Hilber et al. Kinetic properties of myosin heavy chain isoforms in single fibers from human skeletal muscle
DE60136335D1 (de) Parallele genotypisierung mehrerer patientenproben
DE69330067D1 (de) Analytische testvorrichtung einschliesslich negativ- und positivkontrolle
CY1107490T1 (el) Μεθοδος για την ταυτοποιηση δειγματος σε θηλαστικα καθως και κιτ για την εκτελεση αυτης της μεθοδου
BRPI0114343B1 (pt) MÉTODO PARA DETECÇÃO DE IMUNOGLOBULINA IgE E PARA DIAGNÓSTICO IN VITRO DE ALERGIAS, BEM COMO CHIP DE MICROARRANJO E KIT QUE O COMPREENDE
AR243600A1 (es) Un aparato para el tratamiento de muestras biologicas y microbiologicas.
BR0007223A (pt) Dispositivo de teste, e, processos para construirum aparelho de teste de reação de amplificação deácido nucléico em dose unitária e para executaruma reação de amplificação de ácido nucleico
BR9810263A (pt) Processo para avaliar artigos absorventes
DE59609347D1 (de) Verfahren zur quantifizierung von tumorzellen in einer körperflüssigkeit und dazu geeignete testkits
DE69822721D1 (de) Vorrichtung für klinische Analysen
ES2119376T3 (es) Ensayo de enlace.
ATE420204T1 (de) Verfahren zur detektion von erkrankungen
DE69835010D1 (de) Feststellung von endometriumkrebs
DK0943097T3 (da) Fremgangsmåde til at påvise IgE
DE69401717D1 (de) Verfahren zur Aktivierung von polyanionischen,fluoreszierenden Farbstoffen in schwach dielektrischen Medien mit quaternären Oniumverbindungen
PT1181348E (pt) Aparelho e processo para testar um fluido biologico
ES2194116T3 (es) Procedimiento para detectar anticuerpos antipolimero y kit de prueba de diagnostico para uso como ayuda al diagnostico de las enfermedades relacionadas con silicona.
BRPI0001536A (pt) método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp
ES2154736T3 (es) Metodo de analisis.
ATE333510T1 (de) Enzymatische messung von mycophenolsäure
AU2001263771A1 (en) An assay method and kit for testing biological material for exposure to stress using biomarkers

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 594959

Country of ref document: ES