ES2140390T3 - Metodos para la trans-desestabilizacion de proteinas in vivo. - Google Patents

Metodos para la trans-desestabilizacion de proteinas in vivo.

Info

Publication number
ES2140390T3
ES2140390T3 ES91910688T ES91910688T ES2140390T3 ES 2140390 T3 ES2140390 T3 ES 2140390T3 ES 91910688 T ES91910688 T ES 91910688T ES 91910688 T ES91910688 T ES 91910688T ES 2140390 T3 ES2140390 T3 ES 2140390T3
Authority
ES
Spain
Prior art keywords
protein
peptide
interest
methods
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91910688T
Other languages
English (en)
Inventor
Alexander J Varshavsky
Erica S Johnson
David K Gonda
Mark Hochstrasser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Application granted granted Critical
Publication of ES2140390T3 publication Critical patent/ES2140390T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Accessory Devices And Overall Control Thereof (AREA)
  • Control Or Security For Electrophotography (AREA)
  • Facsimiles In General (AREA)

Abstract

ESTE INVENTO CONCIERNE A LA DESESTABILIZACION METABOLICA DE UNA PROTEINA O PEPTIDO DE INTERES IN VIVO. LA PROTEINA O PEPTIDO DE INTERES CONTIENE UN SEGUNDO DETERMINANTE DE LA SEÑAL DE DEGRADACION BASADA EN REGLAS DE EXTREMO N. LOS METODOS DEL INVENTO COMPRENDEN CONTACTAR LA PROTEINA O PEPTIDO DE INTERES CON UNA PROTEINA O PEPTIDO OBJETIVO QUE INTERACCIONA ESPECIFICAMENTE CON LA PROTEINA O PEPTIDO DE INTERES. LA PROTEINA O PEPTIDO OBJETIVO CONTIENE UN ACIDO AMINO TERMINAL AMINO DESESTABILIZADOR DE ACUERDO CON LA REGLA DE EXTREMO N DE LA DEGRADACION DE PROTEINAS, PERO NO TIENE UN SEGUNDO DETERMINANTE DE LA SEÑAL DE DEGRADACION BASADA EN REGLAS DE EXTREMO N. EL METODO EXIGE QUE SEA NECESARIO IDENTIFICAR UN PEPTIDO O PROTEINA QUE INTERACTUA ESPECIFICAMENTE CON LA PROTEINA O PEPTIDO DE INTERES. YA QUE CASI TODAS LAS PROTEINAS INTERACCIONAN ESPECIFICAMENTE CON OTRAS PROTEINAS, ESTE ES UN METODO APLICABLE AMPLIAMENTE PARA DESESTABILIZAR METABOLICAMENTE UNA PROTEINA O PEPTIDO DE INTERES EN VIVO.
ES91910688T 1990-05-17 1991-05-14 Metodos para la trans-desestabilizacion de proteinas in vivo. Expired - Lifetime ES2140390T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/525,150 US5122463A (en) 1990-05-17 1990-05-17 Methods for trans-destabilization of specific proteins in vivo and dna molecules useful therefor

Publications (1)

Publication Number Publication Date
ES2140390T3 true ES2140390T3 (es) 2000-03-01

Family

ID=24092138

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91910688T Expired - Lifetime ES2140390T3 (es) 1990-05-17 1991-05-14 Metodos para la trans-desestabilizacion de proteinas in vivo.

Country Status (10)

Country Link
US (1) US5122463A (es)
EP (1) EP0532587B1 (es)
JP (1) JP3406314B2 (es)
AT (1) ATE188994T1 (es)
CA (1) CA2079584C (es)
DE (1) DE69131929T2 (es)
DK (1) DK0532587T3 (es)
ES (1) ES2140390T3 (es)
GR (1) GR3033094T3 (es)
WO (1) WO1991018096A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6068994A (en) * 1989-08-07 2000-05-30 Chiron Corporation Ubiquitin expression system
US5565352A (en) * 1993-11-24 1996-10-15 Arch Development Corporation Deubiquitinating enzyme: compositions and methods
US5763212A (en) * 1994-02-04 1998-06-09 California Institute Of Technology Inhibiting degradation of a degron-bearing protein
US5538862A (en) * 1994-02-04 1996-07-23 California Institute Of Technology Heat-inducible N-degron module
US6551618B2 (en) 1994-03-15 2003-04-22 University Of Birmingham Compositions and methods for delivery of agents for neuronal regeneration and survival
AU5132996A (en) * 1995-03-01 1996-09-18 Alexander Varshavsky Codominance-mediated toxins
US5932425A (en) * 1997-02-18 1999-08-03 Signal Pharmaceuticals, Inc. Compositions and methods for modulating cellular NF-κB activation
US7157418B1 (en) 1998-07-22 2007-01-02 Osprey Pharmaceuticals, Ltd. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
US20030215421A1 (en) * 1999-07-21 2003-11-20 Mcdonald John R. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
AU2605600A (en) * 1999-01-11 2000-08-01 Mindset Biopharmaceuticals (Usa) Inc. Methods for regulating the stability of recombinant proteins and products usefultherein
US6306663B1 (en) 1999-02-12 2001-10-23 Proteinex, Inc. Controlling protein levels in eucaryotic organisms
US7262005B1 (en) * 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
RU2420537C2 (ru) 2001-01-17 2011-06-10 Трабьон Фармасьютикалз Инк. Слитые белки связывающий домен-иммуноглобулин
US7829084B2 (en) 2001-01-17 2010-11-09 Trubion Pharmaceuticals, Inc. Binding constructs and methods for use thereof
AUPR446801A0 (en) 2001-04-18 2001-05-17 University Of Queensland, The Novel compositions and uses therefor
AU2006284922B2 (en) 2005-08-30 2012-01-19 University Of Miami Immunomodulating tumor necrosis factor receptor 25 (TNFR25) agonists, antagonists and immunotoxins
US8173792B2 (en) 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
JP2011500036A (ja) * 2007-10-15 2011-01-06 ザ ユニバーシティー オブ クイーンズランド 構築物系およびその使用
US8530636B2 (en) 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
JP2012507565A (ja) 2008-10-30 2012-03-29 グオ・ペイシュエン Ｄｎａのシークエンシング及び他の用途のための、膜に組み込まれたウイルスｄｎａパッケージングモータタンパク質コネクタバイオセンサ
SG10201502330TA (en) 2009-08-03 2015-05-28 Univ Miami Method for in vivo expansion of t regulatory cells
US9795658B2 (en) 2010-04-20 2017-10-24 Admedus Vaccines Pty Ltd Expression system for modulating an immune response
US9212359B2 (en) 2011-06-23 2015-12-15 Sanford Jay Silverman Systems for induction of gene expression and protein depletion in yeast
US20140193410A1 (en) 2013-01-09 2014-07-10 University Of Miami Compositions and Methods for the Regulation of T Regulatory Cells Using TL1A-Ig Fusion Protein
WO2018018082A1 (en) 2016-07-26 2018-02-01 The Australian National University Immunostimulatory compositions and uses therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1266639A (en) * 1985-10-16 1990-03-13 Ronald E. Aleshire Cable-pulling system
US5132213A (en) * 1986-10-02 1992-07-21 Massachusetts Institute Of Technology Method for producing proteins and polypeptides using ubiquitin fusions
DE3731103C1 (de) * 1987-09-16 1988-11-24 Schoeller & Co Elektrotech Aus Tastenschaltern zusammengesetztes Tastenfeld

Also Published As

Publication number Publication date
JP3406314B2 (ja) 2003-05-12
CA2079584C (en) 1999-04-13
GR3033094T3 (en) 2000-08-31
CA2079584A1 (en) 1991-11-18
JPH05506360A (ja) 1993-09-22
EP0532587A1 (en) 1993-03-24
WO1991018096A1 (en) 1991-11-28
US5122463A (en) 1992-06-16
DE69131929D1 (de) 2000-02-24
DK0532587T3 (da) 2000-04-17
EP0532587B1 (en) 2000-01-19
DE69131929T2 (de) 2000-08-17
ATE188994T1 (de) 2000-02-15

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