ES2162875T3 - Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos. - Google Patents

Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.

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Publication number
ES2162875T3
ES2162875T3 ES95103520T ES95103520T ES2162875T3 ES 2162875 T3 ES2162875 T3 ES 2162875T3 ES 95103520 T ES95103520 T ES 95103520T ES 95103520 T ES95103520 T ES 95103520T ES 2162875 T3 ES2162875 T3 ES 2162875T3
Authority
ES
Spain
Prior art keywords
ionic detergent
inhibition
enzymes
suppression
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95103520T
Other languages
English (en)
Inventor
Daniel Louis Kacian
Diane Lisa Mcallister
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22789688&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2162875(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ES2162875T3 publication Critical patent/ES2162875T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

UNOS METODOS Y EQUIPOS PARA UTILIZAR DETERGENTES NO IONICOS PARA SUPRIMIR LA INHIBICION ENZIMATICA EN UNA DISOLUCION DE REACCION DEBIDO A LA PRESENCIA DE DETERGENTE IONICO DE INHIBICION. ANTES DE LA REACCION, LA MEZCLA DE REACCION SE DA CON UNA CANTIDAD EFECTIVA DE DETERGENTE NO IONICO Y SE AGITA. LA ENZIMA SE AÑADE ENTONCES, Y LA REACCION ENZIMATICA ES LA PERMITIDA PARA EL PROCESO. TAMBIEN SE DESCRIBEN UNAS EXPRESIONES PREFERIDAS DE LA PRESENTE INVENCION, Y LOS EQUIPOS PARA AMPLIFICACION DE ACIDOS NUCLEICOS DE UNA MUESTRA BIOLOGICA EN PRESENCIA DE UN DETERGENTE IONICO.
ES95103520T 1994-03-10 1995-03-10 Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos. Expired - Lifetime ES2162875T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21213194A 1994-03-10 1994-03-10

Publications (1)

Publication Number Publication Date
ES2162875T3 true ES2162875T3 (es) 2002-01-16

Family

ID=22789688

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95103520T Expired - Lifetime ES2162875T3 (es) 1994-03-10 1995-03-10 Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.

Country Status (11)

Country Link
US (3) US5846701A (es)
EP (1) EP0671473B1 (es)
JP (1) JP3062250B2 (es)
KR (1) KR100238833B1 (es)
AT (1) ATE206465T1 (es)
AU (1) AU699232B2 (es)
CA (1) CA2184270C (es)
DE (1) DE69522978T2 (es)
DK (1) DK0671473T3 (es)
ES (1) ES2162875T3 (es)
WO (1) WO1995024499A1 (es)

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US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
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US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
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US6632557B1 (en) * 1999-10-26 2003-10-14 The Gillette Company Cathodes for metal air electrochemical cells
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US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
AU2000267686A1 (en) * 2000-08-10 2002-02-25 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
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EP1476573B1 (en) * 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2004042003A2 (en) 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
US7364854B2 (en) * 2004-01-23 2008-04-29 Biomerieux, Inc Nucleotide mixture for improved nucleic acid amplification performance
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
CA2584723A1 (en) 2004-10-22 2006-05-04 Redpath Integrated Pathology, Inc. Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna
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US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP1930730B1 (en) 2005-03-10 2019-08-14 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
AU2008235260B2 (en) * 2007-04-05 2013-08-29 Australian Centre For Plant Functional Genomics Pty Ltd Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
EP2152864A1 (en) * 2007-06-13 2010-02-17 Amersham Biosciences Corp. Polymerase stabilization
JP5546109B2 (ja) * 2008-05-27 2014-07-09 富士フイルム株式会社 核酸の塩基配列の識別方法
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
EP2955233A1 (en) 2008-12-19 2015-12-16 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
AU2012222178B2 (en) 2011-02-24 2014-12-18 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
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BR112017013277B1 (pt) * 2014-12-23 2023-02-23 3M Innovative Properties Company Composição aquosa e método de amplificação isotérmicade ácido nucleico
CN108350407B (zh) 2015-12-11 2022-07-08 杰诺玛迪克斯公司 用于核酸扩增的管密封系统和方法
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Also Published As

Publication number Publication date
AU2097895A (en) 1995-09-25
ATE206465T1 (de) 2001-10-15
DE69522978D1 (de) 2001-11-08
US5846701A (en) 1998-12-08
CA2184270A1 (en) 1995-09-14
JPH09510099A (ja) 1997-10-14
US5766890A (en) 1998-06-16
EP0671473B1 (en) 2001-10-04
WO1995024499A1 (en) 1995-09-14
DE69522978T2 (de) 2002-04-04
KR100238833B1 (ko) 2000-03-02
US5871975A (en) 1999-02-16
CA2184270C (en) 1999-08-17
DK0671473T3 (da) 2001-10-29
AU699232B2 (en) 1998-11-26
JP3062250B2 (ja) 2000-07-10
KR970701787A (ko) 1997-04-12
EP0671473A1 (en) 1995-09-13

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