ES2144846T3 - Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos. - Google Patents

Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos.

Info

Publication number
ES2144846T3
ES2144846T3 ES97905634T ES97905634T ES2144846T3 ES 2144846 T3 ES2144846 T3 ES 2144846T3 ES 97905634 T ES97905634 T ES 97905634T ES 97905634 T ES97905634 T ES 97905634T ES 2144846 T3 ES2144846 T3 ES 2144846T3
Authority
ES
Spain
Prior art keywords
nucleic acid
methods
sequence
labels
acid molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97905634T
Other languages
English (en)
Other versions
ES2144846T5 (es
Inventor
Ness Jeffrey Van
John C Tabone
J Jeffry Howbert
John T Mulligan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen Genomics Inc
Original Assignee
Qiagen Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26681206&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2144846(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Qiagen Genomics Inc filed Critical Qiagen Genomics Inc
Application granted granted Critical
Publication of ES2144846T3 publication Critical patent/ES2144846T3/es
Publication of ES2144846T5 publication Critical patent/ES2144846T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PROPORCIONAN METODOS Y COMPUESTOS, INCLUYENDO COMPOSICIONES CONTENIENDO LOS MISMOS, PARA DETERMINAR LA SECUENCIA DE MOLECULAS DE ACIDOS NUCLEICOS. LOS METODOS PERMITEN LA DETERMINACION DE MULTIPLES SECUENCIAS DE ACIDOS NUCLEICOS SIMULTANEAMENTE. LOS COMPUESTOS SE UTILIZAN COMO ETIQUETAS PARA GENERAR FRAGMENTOS DE ACIDOS NUCLEICOS ETIQUETADOS QUE SON COMPLEMENTARIOS A UNA MOLECULA SE ACIDO NUCLEICO SELECCIONADA. CADA ETIQUETA ES CORRELATIVA CON UN DETERMINADO NUCLEOTIDO Y, EN UNA REALIZACION PREFERIDA, PUEDE DETECTARSE POR ESPECTROMETRIA DE MASAS. DESPUES DE LA SEPARACION DE LOS FRAGMENTOS ETIQUETADOS POR SU LONGITUD SECUENCIAL, LAS ETIQUETAS SE ESCINDEN DE LOS FRAGMENTOS ETIQUETADOS. EN UNA REALIZACION PREFERIDA, LAS ETIQUETAS SE DETECTAN MEDIANTE ESPECTROMETRIA DE MASAS Y LA SECUENCIA DE LA MOLECULA DE ACIDO NUCLEICO SE DETERMINA A PARTIR DE ESTAS. LOS PASOS INDIVIDUALES DE LOS METODOS PUEDEN UTILIZARSE EN FORMA AUTOMATICA, P. EJ., MEDIANTE SU INCORPORACION EN SISTEMAS.
ES97905634T 1996-01-23 1997-01-23 Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos. Expired - Lifetime ES2144846T5 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US1046296P 1996-01-23 1996-01-23
US58926096A 1996-01-23 1996-01-23
US10462P 1996-01-23
US589260 1996-01-23

Publications (2)

Publication Number Publication Date
ES2144846T3 true ES2144846T3 (es) 2000-06-16
ES2144846T5 ES2144846T5 (es) 2006-07-16

Family

ID=26681206

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97905634T Expired - Lifetime ES2144846T5 (es) 1996-01-23 1997-01-23 Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos.

Country Status (17)

Country Link
EP (1) EP0868535B9 (es)
JP (2) JP2001501449A (es)
KR (1) KR100482917B1 (es)
CN (1) CN1163619C (es)
AT (2) ATE425175T1 (es)
AU (1) AU717435B2 (es)
BR (1) BR9707056B1 (es)
CA (1) CA2243560A1 (es)
CZ (1) CZ218498A3 (es)
DE (2) DE69701671T3 (es)
ES (1) ES2144846T5 (es)
GR (1) GR3033733T3 (es)
HU (1) HUP9901321A3 (es)
NZ (1) NZ331044A (es)
PL (1) PL328271A1 (es)
PT (1) PT868535E (es)
WO (1) WO1997027331A2 (es)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
DK0840804T3 (da) * 1996-01-23 2000-07-17 Rapigene Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker
DE69700902T2 (de) * 1996-01-23 2000-07-20 Rapigene, Inc. Verfahren zur erkennung von ligandenpaar bindung mit erhöter empfindlichkeit
EP0963443B1 (en) 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
AU6186398A (en) * 1997-02-24 1998-09-09 Tm Technologies, Inc. Process for selecting anti-sense oligonucleotides
EP1002128A1 (en) 1997-07-11 2000-05-24 Brax Genomics Limited Characterising nucleic acids
CA2297158C (en) * 1997-07-22 2009-12-22 Rapigene Inc. Methods and compounds for analyzing nucleic acids by mass spectrometry
EP1000176A1 (en) 1997-07-22 2000-05-17 Rapigene, Inc. Computer method and system for correlating sequencing data by ms
GB9718921D0 (en) 1997-09-05 1997-11-12 Brax Genomics Ltd Catalytically generated mass labels
CA2303790A1 (en) * 1997-09-15 1999-03-25 Brax Group Limited Characterising nucleic acid by mass spectrometry
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
GB9815163D0 (en) * 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds
GB2335035B (en) * 1998-03-03 2003-05-28 Brax Genomics Ltd Screening for functional antisense agents
CA2325399A1 (en) * 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
ATE256142T1 (de) 1998-05-15 2003-12-15 Isis Innovation Bibliotheken von unterschiedlich markierten oligomeren
US6074831A (en) * 1998-07-09 2000-06-13 Agilent Technologies, Inc. Partitioning of polymorphic DNAs
US6270976B1 (en) * 1998-09-15 2001-08-07 Brax Group Limited Characterizing nucleic acid by mass spectrometry
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
KR20020037042A (ko) * 1999-08-31 2002-05-17 퀴아젠 지노믹스 인크. 광절단가능한 결합(들)을 갖는 에난티오머적으로-농축된화합물 및 이와 관련된 방법
GB0006141D0 (en) 2000-03-14 2000-05-03 Brax Group Ltd Mass labels
US6716634B1 (en) 2000-05-31 2004-04-06 Agilent Technologies, Inc. Increasing ionization efficiency in mass spectrometry
DE10036743A1 (de) * 2000-07-27 2002-02-07 Max Planck Gesellschaft Verfahren zur Bestimmung einer partiellen Nukleotidsequenz eines Nukleinsäuremoleküls
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
EP1950305A1 (en) 2001-05-09 2008-07-30 Monsanto Technology, LLC Tyr a genes and uses thereof
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
AU2002327236A1 (en) 2001-07-12 2003-01-29 Illumina, Inc. Multiplex nucleic acid reactions
PT1425586E (pt) * 2001-09-14 2007-12-31 Electrophoretics Ltd Marcadores de massa
US20030165935A1 (en) * 2001-11-21 2003-09-04 Vann Charles S. Digital assay
IL160869A0 (en) 2004-03-15 2004-08-31 Dnr Imaging System Ltd Integrated system for real time gel electrophoresis running and documenting
CN1961070A (zh) 2004-04-22 2007-05-09 泰勒克里斯生物治疗学公司 重组修饰的纤溶酶
US20070054345A1 (en) 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
US20070048752A1 (en) 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
WO2006017208A1 (en) * 2004-07-12 2006-02-16 Applera Corporation Mass tags for quantitative analyses
US20060183238A1 (en) 2005-02-09 2006-08-17 Applera Corporation Amine-containing compound analysis methods
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
GB0515323D0 (en) 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
GB0518585D0 (en) 2005-09-12 2005-10-19 Electrophoretics Ltd Mass labels
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
GB0704764D0 (en) 2007-03-12 2007-04-18 Electrophoretics Ltd Isobarically labelled reagents and methods of their use
US8017403B2 (en) * 2007-06-14 2011-09-13 Quest Diagnostics Investments Incorporated Mass spectrometry method for measuring vitamin B6 in body fluid
JP5539896B2 (ja) 2007-11-29 2014-07-02 グリフオルス・セラピユーテイクス・インコーポレーテツド 組換え的に改変されたプラスミン
GB0809488D0 (en) 2008-05-23 2008-07-02 Electrophoretics Ltd Mass spectrometric analysis
WO2009149199A2 (en) 2008-06-04 2009-12-10 Talecris Biotherapeutics, Inc. Composition, method and kit for preparing plasmin
PT2403865E (pt) 2009-03-03 2015-11-18 Grifols Therapeutics Inc Métodos de preparação de plasminogénio
WO2011060242A2 (en) 2009-11-13 2011-05-19 Talecris Biotherapeutics, Inc. Von willebrand factor (vwf)-containing preparations, and methods, kits, and uses related thereto
GB0921665D0 (en) 2009-12-10 2010-01-27 Trillion Genomics Ltd Phobes
ES2676183T3 (es) 2010-07-02 2018-07-17 Ventana Medical Systems, Inc. Detección de dianas usando marcas de masa y espectrometría de masas
EP2752668A3 (en) 2010-07-23 2014-10-15 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
WO2012026743A2 (ko) 2010-08-23 2012-03-01 포항공과대학교 산학협력단 라벨링제 및 이를 이용한 아미노산 서열 및 단백질 다중 정량 동시 분석방법
KR101207742B1 (ko) 2010-10-14 2012-12-03 포항공과대학교 산학협력단 라벨링제 및 이를 이용한 아미노산 서열 및 단백질 다중 정량 동시 분석방법
CN102140524A (zh) * 2011-03-23 2011-08-03 山东农业大学 多微卫星位点检测试剂盒及检测方法
WO2013070755A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Centrifuge system and workflow
KR20140091032A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 수송 시스템의 자기 감쇠
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
ES2778054T3 (es) 2011-11-07 2020-08-07 Beckman Coulter Inc Sistema y método para transportar recipientes de muestras
CN104040357B (zh) 2011-11-07 2016-11-23 贝克曼考尔特公司 等分器系统以及工作流
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
WO2013093760A2 (en) 2011-12-19 2013-06-27 Grifols, S.A. Compositions, methods, and kits for preparing sialylated recombinant proteins
CN104245947B (zh) 2012-02-29 2018-01-05 杜克大学 用于对映选择性反应的新的氧化还原酶
CN103849617A (zh) * 2013-12-16 2014-06-11 复旦大学 用于基因组dna永久保存的接头及方法
GB201322567D0 (en) 2013-12-19 2014-02-05 Electrophoretics Ltd Mass labels
CN103966210B (zh) * 2014-05-29 2016-06-29 江苏省农业科学院 桃ssap分子标记引物组合、分子标记组合及其在桃品种遗传多样性分析上的应用
WO2016049531A1 (en) 2014-09-26 2016-03-31 Purecircle Usa Inc. Single nucleotide polymorphism (snp) markers for stevia
US10598594B2 (en) 2015-12-22 2020-03-24 University Of Maryland Cell classification based on mechanical signature of nucleus
US10732092B2 (en) 2015-12-22 2020-08-04 University Of Maryland, College Park Analysis of single cell mechanical phenotyping for metastatic detection
JP7138050B2 (ja) 2015-12-22 2022-09-15 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド ブリルアン光散乱に基づくラベルフリーサイトメトリーのシステム及び方法
WO2018170436A1 (en) 2017-03-16 2018-09-20 Jacobs Farm Del Cabo Basil with high tolerance to downy mildew
CN106905399B (zh) * 2017-03-27 2019-07-09 武汉大学 一种双链dna b-z构象相互转换的光控方法
EP3635373B1 (en) * 2017-06-06 2025-07-30 University of Maryland, College Park Analysis of single cell mechanical phenotyping for metastatic detection
US12091706B2 (en) * 2018-01-30 2024-09-17 Rush University Medical Center Sequential staining for multiplex analyses of tissues and cells
US12240813B2 (en) 2020-05-19 2025-03-04 Cybin Irl Limited Deuterated tryptamine derivatives and methods of use
CN112229892B (zh) * 2020-10-22 2021-09-28 中国科学院化学研究所 一种酚醛树脂组成测试和定量分析方法及应用
CN114984032B (zh) * 2022-06-27 2023-07-07 四川大学 Dna四面体框架核酸-绿原酸复合物及其在制备治疗肝纤维化的药物中的用途
CN115337916B (zh) * 2022-07-29 2023-10-24 福建卫生职业技术学院 一种水凝胶型细胞膜半制备色谱柱及其制备方法与应用
CN117269287A (zh) * 2023-11-20 2023-12-22 中国计量科学研究院 一种喷墨进样-毛细管电泳-质谱联用装置及工作方法
EP4667939A1 (en) 2024-06-21 2025-12-24 Commissariat à l'Energie Atomique et aux Energies Alternatives Multiplex cancer cell profiling using ms-tagged probes containing an iminosydnone cleavable linker
CN119119459B (zh) * 2024-09-05 2025-09-09 河南大学 一种以聚酰胺衍生物为内核的仿生金属酶及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5346670A (en) * 1986-12-24 1994-09-13 British Technology Group U.S.A. Inc. Phthalocyanine and tetrabenztriazaporphyrin reagents
US5003059A (en) † 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
DE69433811T2 (de) * 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
EP0765401B1 (en) * 1993-11-17 2001-02-21 Amersham Pharmacia Biotech UK Limited Primer extension mass spectroscopy nucleic acid sequencing method
WO1995025737A1 (en) * 1994-03-23 1995-09-28 The Penn State Research Foundation Method for identifying members of combinatorial libraries

Also Published As

Publication number Publication date
ATE191750T1 (de) 2000-04-15
BR9707056B1 (pt) 2009-05-05
ES2144846T5 (es) 2006-07-16
AU717435B2 (en) 2000-03-23
KR100482917B1 (ko) 2005-11-11
ATE425175T1 (de) 2009-03-15
DE69701671T3 (de) 2006-08-17
DE69701671D1 (de) 2000-05-18
CZ218498A3 (cs) 1998-12-16
EP0868535B9 (en) 2007-05-09
EP0868535A2 (en) 1998-10-07
EP0868535B1 (en) 2000-04-12
JP2001501449A (ja) 2001-02-06
NZ331044A (en) 1999-02-25
CN1163619C (zh) 2004-08-25
BR9707056A (pt) 1999-12-28
DE69701671T2 (de) 2000-08-10
PL328271A1 (en) 1999-01-18
DE69739304D1 (de) 2009-04-23
JP2008183012A (ja) 2008-08-14
HUP9901321A2 (hu) 1999-07-28
HUP9901321A3 (en) 2001-10-29
CN1212021A (zh) 1999-03-24
CA2243560A1 (en) 1997-07-31
PT868535E (pt) 2000-12-29
WO1997027331A3 (en) 1998-04-02
GR3033733T3 (en) 2000-10-31
KR19990081925A (ko) 1999-11-15
EP0868535B2 (en) 2006-01-04
AU2247397A (en) 1997-08-20
WO1997027331A2 (en) 1997-07-31

Similar Documents

Publication Publication Date Title
ES2144846T3 (es) Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos.
ES2145580T3 (es) Metodos y composiciones para analizar moleculas de acido nucleico que emplean tecnicas basadas en el tamaño.
US5302509A (en) Method for sequencing polynucleotides
DE59510976D1 (de) Verfahren zur isolierung von zellinhaltsstoffen, wie nucleinsäuren, aus natürlichen quellen
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
CO4780063A1 (es) Un sustema y metodos para procesar de forma dinamica un indi ce para crear de forma dinamica una serie de preguntas.
MX9400837A (es) Metodos y aparatos para la determinacion de secuencia de adn.
ATE257291T1 (de) Signalangepasstes filter unter verwendung von vorgegebener kombinationen im zeitmultiplex- verfahren
ATE250671T1 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
CY1108920T1 (el) Χαρακτηρισμος της γονιδιακης λειτουργιας με τη χρηση αναστολης δικλωνου rna
ATE414783T1 (de) Verfahren zur isolierung von stammzellen
WO2005059157A3 (en) METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
ATA44185A (de) Verfahren zur herstellung von nucleinsaeure-reagenzien
DE59708838D1 (de) Spiegelselektion und spiegelevolution von nucleinsäuren
ATE409236T1 (de) Derivatisierte nukleinsäuren und deren verwendung
ATE541924T1 (de) Selektion und isolierung lebender zellen unter verwendung rna-bindender sonden
DE59704288D1 (de) Verfahren zur Herstellung von Alpha,Omega-C4- bis C20-Alkenolen
DK1019420T3 (da) Fremgangsmåder til fremstilling af forbindelsesled på grundlag af platin mellem markører og bioorganiske molekyler til mærkning af bioorganiske molekyler til påvisning af interessante biologiske stoffer
GB2377272A (en) Method and apparatus for DNA sequencing
AR012633A1 (es) Un metodo para la produccion de numerosos neumaticos y un sistema relacionado para su produccion
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
DE59812184D1 (de) Verfahren zur identifikation von nucleinsäuren durch elektrospray massenspektrometrie
ES2083629T3 (es) Inmovilizacion de acidos nucleicos.
DK1027669T3 (da) Fremgangsmåde til indgruppering af sekvenser i familier
DE50204046D1 (de) Verfahren zur trennung einer in einem bild einer probe enthaltenen zellgruppe in einzelzellen

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 868535

Country of ref document: ES