ES2156510B1 - Un procedimiento para la produccion de acido 2-hidroxifenilacetico utilizando mutantes de la bacteria pseudomonas putida u asi como una cepa recombinante de e.coli en la que se han introducido genes aislados de pseudomonas putida. - Google Patents
Un procedimiento para la produccion de acido 2-hidroxifenilacetico utilizando mutantes de la bacteria pseudomonas putida u asi como una cepa recombinante de e.coli en la que se han introducido genes aislados de pseudomonas putida.Info
- Publication number
- ES2156510B1 ES2156510B1 ES9802352A ES9802352A ES2156510B1 ES 2156510 B1 ES2156510 B1 ES 2156510B1 ES 9802352 A ES9802352 A ES 9802352A ES 9802352 A ES9802352 A ES 9802352A ES 2156510 B1 ES2156510 B1 ES 2156510B1
- Authority
- ES
- Spain
- Prior art keywords
- pseudomonas
- mutants
- pseudomonas putida
- coli
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- CCVYRRGZDBSHFU-UHFFFAOYSA-N (2-hydroxyphenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC=C1O CCVYRRGZDBSHFU-UHFFFAOYSA-N 0.000 title abstract 6
- 241000589776 Pseudomonas putida Species 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 241000589516 Pseudomonas Species 0.000 title 1
- WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 abstract 4
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 230000036983 biotransformation Effects 0.000 abstract 2
- 229960003424 phenylacetic acid Drugs 0.000 abstract 2
- 239000003279 phenylacetic acid Substances 0.000 abstract 2
- WLJVXDMOQOGPHL-PPJXEINESA-N 2-phenylacetic acid Chemical compound O[14C](=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-PPJXEINESA-N 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 230000006652 catabolic pathway Effects 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Un procedimiento para la producción de ácido 2- hidroxifenilacético utilizando mutantes de la bacteria Pseudomonas putida U así como una cepa recombinante de E.coli en la que se han introducido genes aislados de Pseudomonas putida. La invención que se presenta, describe un procedimiento para la biotransformación del ácido fenilacético (PA) en ácido 2- hidroxifenilacético (abreviadamente indicado como 2-HPA), utilizando mutantes de la bacteria Pseudomonas putida U interrumpidos en uno de los genes (phaL) que compone la ruta catabólica de ácido fenilacético, así como el empleo de una cepa de E.coli recombinante en la que se han introducido, y se expresan, los.genes aislados de Pseudomonas putida que codifican las enzimas responsables de esa biotransformación.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9802352A ES2156510B1 (es) | 1998-11-11 | 1998-11-11 | Un procedimiento para la produccion de acido 2-hidroxifenilacetico utilizando mutantes de la bacteria pseudomonas putida u asi como una cepa recombinante de e.coli en la que se han introducido genes aislados de pseudomonas putida. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9802352A ES2156510B1 (es) | 1998-11-11 | 1998-11-11 | Un procedimiento para la produccion de acido 2-hidroxifenilacetico utilizando mutantes de la bacteria pseudomonas putida u asi como una cepa recombinante de e.coli en la que se han introducido genes aislados de pseudomonas putida. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2156510A1 ES2156510A1 (es) | 2001-06-16 |
| ES2156510B1 true ES2156510B1 (es) | 2002-01-16 |
Family
ID=8305732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES9802352A Expired - Fee Related ES2156510B1 (es) | 1998-11-11 | 1998-11-11 | Un procedimiento para la produccion de acido 2-hidroxifenilacetico utilizando mutantes de la bacteria pseudomonas putida u asi como una cepa recombinante de e.coli en la que se han introducido genes aislados de pseudomonas putida. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2156510B1 (es) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4232522A1 (de) * | 1992-09-29 | 1994-03-31 | Basf Ag | Verfahren zur fermentativen Herstellung von 2-Hydroxyphenylessigsäure |
-
1998
- 1998-11-11 ES ES9802352A patent/ES2156510B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2156510A1 (es) | 2001-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20010616 Kind code of ref document: A1 Effective date: 20010616 |
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| FD2A | Announcement of lapse in spain |
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