ES2158895T3 - Metodo para detectar un acido nucleico diana. - Google Patents

Metodo para detectar un acido nucleico diana.

Info

Publication number
ES2158895T3
ES2158895T3 ES94916811T ES94916811T ES2158895T3 ES 2158895 T3 ES2158895 T3 ES 2158895T3 ES 94916811 T ES94916811 T ES 94916811T ES 94916811 T ES94916811 T ES 94916811T ES 2158895 T3 ES2158895 T3 ES 2158895T3
Authority
ES
Spain
Prior art keywords
nucleic acid
primer
detecting
target nucleic
priming sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94916811T
Other languages
English (en)
Inventor
Chang-Ning J Wang
Kai-Yuan Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotronics Corp
Original Assignee
Biotronics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotronics Corp filed Critical Biotronics Corp
Application granted granted Critical
Publication of ES2158895T3 publication Critical patent/ES2158895T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

UN METODO PARA DETECTAR UN ACIDO NUCLEICO DIANA, EL CUAL INCLUYE LOS PASOS DE (I) AMPLIFICAR EL ACIDO NUCLEICO DIANA PARA OBTENER UN PRODUCTO DE AMPLIFICACION MEDIANTE UNA POLIMERASA, UN PRIMER INICIADOR CON O SIN UN SEGMENTO NO CONTIGUO A UNA PRIMERA SECUENCIA DE INICIO, Y UN SEGUNDO INICIADOR CON O SIN UN SEGMENTO NO CONTIGUO A UNA SEGUNDA SECUENCIA DE INICIO EN PRESENCIA DE UN OLIGONUCLEOTIDO QUE ES INCAPAZ DE ACTUAR COMO UN INICIADOR PARA LA POLIMERASA, EN DONDE EL OLIGONUCLEOTIDO TIENE AL MENOS 5 NUCLEOTIDOS CONSECUTIVOS COMPLETAMENTE COMPLEMENTARIOS A AL MENOS 5 NUCLEOTIDOS CONSECUTIVOS DEL PRIMER INICIADOR, Y (II) DETECTAR LA PRESENCIA DEL ACIDO NUCLEICO DIANA MEDIANTE LA SUPERVISION DE LA AMPLIFICACION DEL MISMO. EN CONCRETO, LA FIGURA ILUSTRA EL METODO EN DONDE EL INICIADOR CONTIENE UN SEGMENTO NO CONTIGUO A SU SECUENCIA DE INICIO.
ES94916811T 1994-05-23 1994-05-23 Metodo para detectar un acido nucleico diana. Expired - Lifetime ES2158895T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BR9408578A BR9408578A (pt) 1994-05-23 1994-05-23 Método para detecção de um ácido nucléico alvo
PCT/US1994/005767 WO1995032306A1 (en) 1994-05-23 1994-05-23 Method for detecting a target nucleic acid

Publications (1)

Publication Number Publication Date
ES2158895T3 true ES2158895T3 (es) 2001-09-16

Family

ID=40549889

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94916811T Expired - Lifetime ES2158895T3 (es) 1994-05-23 1994-05-23 Metodo para detectar un acido nucleico diana.

Country Status (12)

Country Link
EP (1) EP0763133B1 (es)
JP (1) JPH10500572A (es)
AT (1) ATE203775T1 (es)
AU (1) AU706033B2 (es)
BR (1) BR9408578A (es)
DE (1) DE69427876T2 (es)
DK (1) DK0763133T3 (es)
ES (1) ES2158895T3 (es)
GR (1) GR3037030T3 (es)
NZ (1) NZ266724A (es)
RU (1) RU2158765C2 (es)
WO (1) WO1995032306A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US7273749B1 (en) 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
EP1442794B1 (en) * 1996-06-04 2006-03-29 University Of Utah Research Foundation System and method for carrying out and monitoring biological processes
DE69739357D1 (de) * 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JP4185185B2 (ja) * 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖ｄｎａを利用したｄｎａの検出方法
WO1999042615A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Methods for determining amounts of nucleic acids
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
EP1709204A1 (en) * 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
EP2336354A1 (en) * 2009-12-18 2011-06-22 Roche Diagnostics GmbH A method for the detection of a RNA molecule, a kit and a use related thereof
RU2578009C2 (ru) * 2013-05-08 2016-03-20 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
RU2600873C1 (ru) * 2015-06-24 2016-10-27 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
KR102468839B1 (ko) * 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
SU1678838A1 (ru) * 1989-05-03 1991-09-23 Всесоюзный научно-исследовательский институт биологического приборостроения Способ вы влени нуклеотидных последовательностей
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
KR950013953B1 (ko) * 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
WO1993020227A1 (en) * 1992-03-31 1993-10-14 Abbott Laboratories Method of multiplex ligase chain reaction
AU4788493A (en) * 1992-07-21 1994-02-14 Imclone Systems Incorporated Gap-filling nucleic acid amplification and detection
US6180338B1 (en) * 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences

Also Published As

Publication number Publication date
EP0763133A4 (en) 1999-02-10
EP0763133A1 (en) 1997-03-19
DE69427876D1 (de) 2001-09-06
JPH10500572A (ja) 1998-01-20
EP0763133B1 (en) 2001-08-01
WO1995032306A1 (en) 1995-11-30
AU6836494A (en) 1995-12-18
BR9408578A (pt) 1997-08-19
NZ266724A (en) 1998-08-26
ATE203775T1 (de) 2001-08-15
DE69427876T2 (de) 2002-04-11
GR3037030T3 (en) 2002-01-31
DK0763133T3 (da) 2001-10-08
HK1001131A1 (en) 1998-05-29
AU706033B2 (en) 1999-06-10
RU2158765C2 (ru) 2000-11-10

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