ES2169753T3 - Procedimiento de determinacion de acidos nucleicos mediante amplificacion de los mismos. - Google Patents

Procedimiento de determinacion de acidos nucleicos mediante amplificacion de los mismos.

Info

Publication number
ES2169753T3
ES2169753T3 ES95890172T ES95890172T ES2169753T3 ES 2169753 T3 ES2169753 T3 ES 2169753T3 ES 95890172 T ES95890172 T ES 95890172T ES 95890172 T ES95890172 T ES 95890172T ES 2169753 T3 ES2169753 T3 ES 2169753T3
Authority
ES
Spain
Prior art keywords
target
amplification
determination
procedure
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95890172T
Other languages
English (en)
Inventor
Falko-Gunter Dr Falkner
Thomas Dr Hammerle
Michele Dr Himmelspach
Johann Dr Kohl
Friedrich Prof Dr Dorner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter AG
Original Assignee
Baxter AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT183194A external-priority patent/AT401062B/de
Priority claimed from AT224594A external-priority patent/ATA224594A/de
Application filed by Baxter AG filed Critical Baxter AG
Application granted granted Critical
Publication of ES2169753T3 publication Critical patent/ES2169753T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA LA CUANTIFICACION DE ACIDOS NUCLEICOS EN UNA MUESTRA BAJO UTILIZACION DE AMPLIFICACION DE ACIDO NUCLEICO, DONDE A LA MUESTRA SE AÑADE ANTES DEL PASO DE AMPLIFICACION UNA CANTIDAD DADA DE UNA MOLECULA DE ACIDO NUCLEICO CONOCIDO COMO STANDARD INTERNO, QUE DIFERENCIA LA MOLECULA DE ACIDO NUCLEICO STANDARD DEL ACIDO NUCLEICO A SER CUANTIFICADO AL MENOS EN UNA CARACTERISTICA DETECTABLE. PARA LA OBTENCION DE UNA EXACTITUD ALTA Y BUENA CAPACIDAD DE REPRODUCCION SE PROPONE, QUE A LA MUESTRA SE LE AÑADA ANTES DE LA AMPLIFICACION DE ACIDO NUCLEICO CANTIDADES CONOCIDAS DE AL MENOS DOS MOLECULAS DE ACIDOS NUCLEICOS CONOCIDAS DE FORMA DIFERENTE EN UNA CARACTERISTICA DETECTABLE UNA DE OTRA A PARTIR DEL ACIDO NUCLEICO A SER CUANTIFICADO, COMO STANDARD INTERNO, DE FORMA QUE SE DETERMINA LAS CANTIDADES EN EL ACIDO NUCLEICO STANDARD Y DE MUESTRA AMPLIFICADAS Y SE DETERMINA A PARTIR DE LAS CANTIDADES OBTENIDAS LAS CANTIDADES PRESENTES ORIGINALMENTE EN LA MUESTRA EN EL ACIDO NUCLEICO A SER CUANTIFICADO.
ES95890172T 1994-09-26 1995-09-26 Procedimiento de determinacion de acidos nucleicos mediante amplificacion de los mismos. Expired - Lifetime ES2169753T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT183194A AT401062B (de) 1994-09-26 1994-09-26 Verfahren zur quantifizierung von nukleinsäuren
AT224594A ATA224594A (de) 1994-12-02 1994-12-02 Verfahren zur quantifizierung von nukleinsäuren

Publications (1)

Publication Number Publication Date
ES2169753T3 true ES2169753T3 (es) 2002-07-16

Family

ID=25596921

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95890172T Expired - Lifetime ES2169753T3 (es) 1994-09-26 1995-09-26 Procedimiento de determinacion de acidos nucleicos mediante amplificacion de los mismos.

Country Status (8)

Country Link
US (1) US5789153A (es)
EP (1) EP0714988B1 (es)
JP (1) JPH08107798A (es)
AT (1) ATE210735T1 (es)
CA (1) CA2159044A1 (es)
DE (1) DE59509938D1 (es)
DK (1) DK0714988T3 (es)
ES (1) ES2169753T3 (es)

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US5814442A (en) * 1994-06-10 1998-09-29 Georgetown University Internally controlled virion nucleic acid amplification reaction for quantitation of virion and virion nucleic acid
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US6265152B1 (en) * 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
AT406778B (de) * 1996-04-09 2000-09-25 Immuno Ag Verfahren zur desintegration von nucleinsäuren und herstellung von qualitätsgesicherten biologischen produkten
AT406373B (de) 1997-02-27 2000-04-25 Immuno Ag Verfahren zur reinigung von faktor viii/vwf-komplex mittels kationenaustauscherchromatographie
AT405403B (de) 1997-02-27 1999-08-25 Immuno Ag Reinigung von von willebrand-faktor durch kationenaustauscherchromatographie
DE19752898A1 (de) * 1997-11-28 1999-08-05 Centeon Pharma Gmbh Verfahren zum Nachweis hoher Vierenkonzentrationen im Blutplasma und/oder Blutserum mittels der Polymerasekettenreaktion
AT407160B (de) * 1998-06-04 2001-01-25 Immuno Ag Verfahren zur bestimmung von antigenen
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6379889B1 (en) * 1999-11-04 2002-04-30 Agilent Technologies, Inc. Multiplexing methods for identifying nucleic acids using denaturing liquid chromatography
AT409382B (de) 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion, unterscheidung und quantifizierung von freien und eingekapselten nukleinsäuren in einer probe
EP1319716B1 (en) * 2000-09-22 2013-07-03 Hitachi, Ltd. Method of analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US6982029B2 (en) * 2001-05-07 2006-01-03 Spectramedix Llc Electrophoretic method and system having internal lane standards for color calibration
US20030207310A1 (en) * 2002-05-01 2003-11-06 Zhaowei Liu System and method for determining the sizes and quantity of polynucleotides with capillary array electrophoresis
ATE496141T1 (de) * 2003-12-06 2011-02-15 Abbott Lab Verfahren und system zum analysieren von reaktionen unter verwendung eines informationssystems
EP2412830B1 (en) 2004-07-13 2014-11-19 Gen-Probe Incorporated Compositions and methods for detection of Hepatitis A virus nucleic acid
US7794659B2 (en) 2005-03-10 2010-09-14 Gen-Probe Incorporated Signal measuring system having a movable signal measuring device
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Ｄｎａ定量方法、及び遺伝子解析方法
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
JP6121591B2 (ja) * 2010-07-29 2017-04-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 微生物核酸の定性的および定量的検出
CN107190056A (zh) * 2010-07-29 2017-09-22 霍夫曼-拉罗奇有限公司 微生物核酸的定性和定量检测
WO2012051504A2 (en) 2010-10-14 2012-04-19 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
CN121227955B (zh) * 2025-12-04 2026-03-20 宝瑞源生物技术(北京)有限公司 一种单管定量检测hbv病毒载量实时荧光定量pcr试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
BR9206131A (pt) * 1991-06-10 1995-05-02 Lucky Ltd Hepatite C: diagnóstico e vacinas
JP2739004B2 (ja) * 1992-01-16 1998-04-08 三菱電機株式会社 半導体装置
AU4248193A (en) * 1992-05-15 1993-12-13 New England Deaconess Hospital Quantitation of viral rna by competitive polymerase chain reaction
AU5443794A (en) * 1992-10-30 1994-05-24 Rogosin Institute, The Method for quantifying nucleic acids
AU6359794A (en) * 1993-03-05 1994-09-26 Genelabs Technologies, Inc. Method for hiv quantitation
DK0662156T3 (da) * 1993-07-09 1999-10-25 Akzo Nobel Nv Forbedret fremgangsmåde til kvantificering af nukleinsyre
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Also Published As

Publication number Publication date
DE59509938D1 (de) 2002-01-24
DK0714988T3 (da) 2002-04-08
EP0714988B1 (de) 2001-12-12
EP0714988A3 (de) 1997-04-16
CA2159044A1 (en) 1996-03-27
US5789153A (en) 1998-08-04
ATE210735T1 (de) 2001-12-15
EP0714988A2 (de) 1996-06-05
JPH08107798A (ja) 1996-04-30

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