ES2152995T3 - Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia. - Google Patents

Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia.

Info

Publication number
ES2152995T3
ES2152995T3 ES95104930T ES95104930T ES2152995T3 ES 2152995 T3 ES2152995 T3 ES 2152995T3 ES 95104930 T ES95104930 T ES 95104930T ES 95104930 T ES95104930 T ES 95104930T ES 2152995 T3 ES2152995 T3 ES 2152995T3
Authority
ES
Spain
Prior art keywords
amplification
fluorescence polarization
detection
nucleic acid
fluorometer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95104930T
Other languages
English (en)
Inventor
George Terrance Walker
James Gregory Nadeau
Carl Preston Linn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26923151&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2152995(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US08/229,281 external-priority patent/US5547861A/en
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2152995T3 publication Critical patent/ES2152995T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Lasers (AREA)

Abstract

METODOS DE POLARIZACION FLUORESCENTE PARA LA DETECCION DE LA AMPLIFICACION DE ACIDOS NUCLEICOS. CONVIRTIENDO UNA SONDA DE OLIGODEOXINUCLEOTIDO MARCADA DE FORMA FLUORESCENTE DE LA FORMA DE UNA HEBRA A LA DE DOBLE HEBRA, UNA FORMA DEPENDIENTE DEL OBJETIVO DURANTE LA AMPLIFICACION DE LA SECUENCIA OBJETIVO. ESTE CAMBIO CONFORMACIONAL VA ACOMPAÑADO DE UN AUMENTO DE LOS VALORES DE LA POLARIZACION FLUORESCENTE. EL AUMENTO DE LA POLARIZACION FLUORESCENTE SE PUEDE MEDIR EN UN FLUOROMETRO EN REGIMEN TRANSITORIO EN TIEMPO REAL DURANTE LA REACCION DE AMPLIFICACION SIN MANIPULAR FISICAMENTE LA MUESTRA. CON LOS METODOS DE LA INVENCION SE CONSIGUE POR TANTO UN SISTEMA CERRADO Y HOMOGENEO TANTO PARA LA AMPLIFICACION DE LAS SECUENCIAS OBJETIVO COMO PARA DETECTAR LA AMPLIFICACION. ALTERNATIVAMENTE, LA AMPLIFICACION SE PUDE DETECTAR EN EL FLUOROMETRO UNA VEZ TERMINADA LA REACCION DE AMPLIFICACION.
ES95104930T 1994-04-18 1995-04-03 Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia. Expired - Lifetime ES2152995T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/229,281 US5547861A (en) 1994-04-18 1994-04-18 Detection of nucleic acid amplification
US08/311,474 US5593867A (en) 1994-04-18 1994-09-23 Fluorerscence polarization detection of nucleic acid amplication

Publications (1)

Publication Number Publication Date
ES2152995T3 true ES2152995T3 (es) 2001-02-16

Family

ID=26923151

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95104930T Expired - Lifetime ES2152995T3 (es) 1994-04-18 1995-04-03 Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia.

Country Status (7)

Country Link
EP (1) EP0678581B1 (es)
JP (1) JP2757979B2 (es)
AT (1) ATE197071T1 (es)
BR (1) BR9501583A (es)
CA (1) CA2145719C (es)
DE (1) DE69519122T2 (es)
ES (1) ES2152995T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550025A (en) * 1995-07-19 1996-08-27 Becton, Dickinson And Company Detection of hydrophobic amplification products by extraction into an organic phase
US5641633A (en) * 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
US5800989A (en) * 1995-11-15 1998-09-01 Becton, Dickinson And Company Method for detection of nucleic acid targets by amplification and fluorescence polarization
WO1997021832A1 (de) * 1995-12-08 1997-06-19 Evotec Biosystems Gmbh Verfahren zur bestimmung von nukleinsäuremolekülen in niedriger konzentration
DE19545965A1 (de) * 1995-12-08 1997-06-12 Max Planck Gesellschaft Verfahren zur Bestimmung von Nukleinsäuremolekülen in niedriger Konzentration
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
EP0822261B1 (en) * 1996-07-29 2002-07-03 Academia Sinica Methods for rapid antimicrobial susceptibility testing
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
JP2002272475A (ja) * 2001-03-22 2002-09-24 Eiken Chem Co Ltd 蛍光偏光法による核酸増幅産物の検出方法
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0275958A (ja) * 1988-09-13 1990-03-15 Kao Corp Ｄｎａ検出法
WO1990006374A1 (en) * 1988-12-09 1990-06-14 Amrad Corporation Limited Amplified dna assay
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
AU8997991A (en) * 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
JPH0643159A (ja) * 1991-02-20 1994-02-18 Toyobo Co Ltd 固定化ｄｎａまたはｒｎａを用いるｄｎａまたはｒｎａ断片の測定方法
EP0533906A4 (en) * 1991-04-11 1994-09-28 Baxter Diagnostics Inc Detection of dna/rna by fluorescence polarization
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1993009250A1 (en) * 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
JP2788786B2 (ja) * 1991-11-15 1998-08-20 イゲン，インコーポレーテッド 増幅生成物の迅速アッセイ
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen

Also Published As

Publication number Publication date
ATE197071T1 (de) 2000-11-15
CA2145719C (en) 1998-06-30
BR9501583A (pt) 1995-11-14
EP0678581B1 (en) 2000-10-18
CA2145719A1 (en) 1995-10-19
EP0678581A1 (en) 1995-10-25
JP2757979B2 (ja) 1998-05-25
DE69519122D1 (de) 2000-11-23
JPH0838199A (ja) 1996-02-13
DE69519122T2 (de) 2001-03-22

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