ES2170204T3 - Complejos de marcado fluorescente con desplazamientos de stokes amplios formados por copulacion de cianina y otros fluorocromos capaces de transferir energia de resonancia. - Google Patents

Complejos de marcado fluorescente con desplazamientos de stokes amplios formados por copulacion de cianina y otros fluorocromos capaces de transferir energia de resonancia.

Info

Publication number
ES2170204T3
ES2170204T3 ES96303879T ES96303879T ES2170204T3 ES 2170204 T3 ES2170204 T3 ES 2170204T3 ES 96303879 T ES96303879 T ES 96303879T ES 96303879 T ES96303879 T ES 96303879T ES 2170204 T3 ES2170204 T3 ES 2170204T3
Authority
ES
Spain
Prior art keywords
complexes
protein
complex
wave length
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96303879T
Other languages
English (en)
Inventor
Alan Stewart Waggoner
Swati Ratnakar Mujumdar
Ratnakar Balvant Mujumdar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carnegie Mellon University
Original Assignee
Carnegie Mellon University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23893630&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2170204(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Carnegie Mellon University filed Critical Carnegie Mellon University
Application granted granted Critical
Publication of ES2170204T3 publication Critical patent/ES2170204T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/22—Amino derivatives of triarylmethanes containing OH groups bound to an aryl nucleus and their ethers and esters
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/06—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups three >CH- groups, e.g. carbocyanines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/10—The polymethine chain containing an even number of >CH- groups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Luminescent Compositions (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

LA PRESENTE INVENCION SUMINISTRA COMPLEJOS DE ETIQUETADO FLUORESCENTES DE BAJO PESO MOLECULAR CON GRANDES DESVIACIONES DE LONGITUD DE ONDA ENTRE LA ABSORCION DE UN TINTE EN EL COMPLEJO Y LA EMISION DE OTRO TINTE EN EL COMPLEJO. ESTOS COMPLEJOS PUEDES UTILIZARSE, POR EJEMPLO, PARA ANALISIS CELULARES DE FLUORESCENCIA DE PARAMETROS MULTIPLES USANDO UNA LONGITUD DE ONDA DE EXCITACION SIMPLE. EL BAJO PESO MOLECULAR DEL COMPLEJO PERMITE QUE LOS MATERIALES ETIQUETADOS CON EL COMPLEJO PENETREN EN LAS ESTRUCTURAS CELULARES PARA SU USO COMO SONDAS. LOS COMPLEJOS DE ETIQUETADO SE SINTETIZAN UNIENDOLOS COVALENTEMENTE A TRAVES DE ENLAZADORES PARA FORMAR COMPLEJOS DE DONANTE-ACEPTANTE. LA TRANSFERENCIA DE ENERGIA DE RESONANCIA DESDE UN DONANTE EXCITADO A UN ACEPTANTE FLUORESCENTE SUMINISTRA CAMBIOS DE LONGITUD DE ONDA DE HASTA 300NM. LOS COMPLEJOS DE ETIQUETADO FLUORESCENTES CONTIENEN PREFERIBLEMENTE GRUPOS REACTIVOS PARA EL ETIQUETADO DE GRUPOS FUNCIONALES SOBRE COMPUESTOS DE OBJETIVO, TALES COMO ACIDOSPOLINUCLEICOS OXI Y DEOXI , ANTICUERPOS, ENZIMAS, LIPIDOS, CARBOHIDRATOS, PROTEINAS Y OTROS MATERIALES. LOS COMPLEJOS PUEDEN CONTENER GRUPOS FUNCIONALES QUE PERMITAN LA REACCION COVALENTE CON MATERIALES QUE CONTENGAN GRUPOS REACTIVOS.
ES96303879T 1995-06-07 1996-05-30 Complejos de marcado fluorescente con desplazamientos de stokes amplios formados por copulacion de cianina y otros fluorocromos capaces de transferir energia de resonancia. Expired - Lifetime ES2170204T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/476,880 US6008373A (en) 1995-06-07 1995-06-07 Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer

Publications (1)

Publication Number Publication Date
ES2170204T3 true ES2170204T3 (es) 2002-08-01

Family

ID=23893630

Family Applications (2)

Application Number Title Priority Date Filing Date
ES99110086T Expired - Lifetime ES2248942T3 (es) 1995-06-07 1996-05-30 Complejos con un gran desplazamiento de stokes para marcado fluorescente formado por copulacion de cianina y otros fluorocromos capares de transferir energia de resonancia.
ES96303879T Expired - Lifetime ES2170204T3 (es) 1995-06-07 1996-05-30 Complejos de marcado fluorescente con desplazamientos de stokes amplios formados por copulacion de cianina y otros fluorocromos capaces de transferir energia de resonancia.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES99110086T Expired - Lifetime ES2248942T3 (es) 1995-06-07 1996-05-30 Complejos con un gran desplazamiento de stokes para marcado fluorescente formado por copulacion de cianina y otros fluorocromos capares de transferir energia de resonancia.

Country Status (8)

Country Link
US (5) US6008373A (es)
EP (2) EP0943918B1 (es)
JP (1) JP2843296B2 (es)
AT (2) ATE302412T1 (es)
CA (1) CA2178308C (es)
DE (2) DE69635089T2 (es)
ES (2) ES2248942T3 (es)
GB (1) GB2301833B (es)

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