ES2171679T3 - Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva. - Google Patents

Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva.

Info

Publication number
ES2171679T3
ES2171679T3 ES96917000T ES96917000T ES2171679T3 ES 2171679 T3 ES2171679 T3 ES 2171679T3 ES 96917000 T ES96917000 T ES 96917000T ES 96917000 T ES96917000 T ES 96917000T ES 2171679 T3 ES2171679 T3 ES 2171679T3
Authority
ES
Spain
Prior art keywords
nucleic acid
target
acid sequence
test sample
resource
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96917000T
Other languages
English (en)
Inventor
Larry Birkenmeyer
Isa K Mushahwar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2171679T3 publication Critical patent/ES2171679T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EN LA PRESENTE INVENCION SE PRESENTA UN METODO PARA DETECTAR DE FORMA CUANTITATIVA LA CANTIDAD DE UNA SECUENCIA DE ACIDO NUCLEICO OBJETIVO QUE SE PUEDE ENCONTRAR PRESENTE EN UNA MUESTRA DE ENSAYO. SE PONE EN CONTACTO UNA MUESTRA DE ENSAYO QUE PUEDE CONTENER UNA SECUENCIA DE ACIDO NUCLEICO OBJETIVO QUE CONTIENE SECUENCIAS OBJETIVO X E Y CON DOS GRUPOS DE IMPRIMADORES, SIENDO EL PRIMER GRUPO ESPECIFICO PARA EL OBJETIVO X Y EL SEGUNDO GRUPO ESPECIFICO PARA EL OBJETIVO Y. LA MUESTRA DE ENSAYO TAMBIEN SE PONE EN CONTACTO AL MISMO TIEMPO CON UNA SECUENCIA ESTANDAR INTERNA IS, DERIVADA SUSTANCIALMENTE DE UNA COMBINACION DE LA PRIMERA Y LA SEGUNDA SECUENCIAS OBJETIVO, Y SUS IMPRIMADORES OLIGONUCLEOTIDOS CORRESPONDIENTES. SE ASOCIAN UNOS HAPTENOS A LOS GRUPOS DE IMPRIMADORES OLIGONUCLEOTIDOS DE MODO QUE LOS PRODUCTOS DE LAS SECUENCIAS OBJETIVO AMPLIFICADAS X E Y SE PUEDAN DETECTAR POR CAPTURA EN UNA FASE SOLIDA A LA QUE ESTAN ACOPLADOS LOS REACTIVOS DE CAPTURA ANTIHAPTENOS. SE DETERMINA UNA RELACIONDE SEÑAL DE (X + Y)/S PARA CUANTIFICAR LA CANTIDAD DE LA SECUENCIA SE ACIDO NUCLEICO OBJETIVO PRESENTE EN LA MUESTRA.
ES96917000T 1995-06-07 1996-06-03 Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva. Expired - Lifetime ES2171679T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/480,220 US5667974A (en) 1995-06-07 1995-06-07 Method for detecting nucleic acid sequences using competitive amplification

Publications (1)

Publication Number Publication Date
ES2171679T3 true ES2171679T3 (es) 2002-09-16

Family

ID=23907134

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96917000T Expired - Lifetime ES2171679T3 (es) 1995-06-07 1996-06-03 Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva.

Country Status (8)

Country Link
US (2) US5667974A (es)
EP (1) EP0832281B1 (es)
JP (1) JPH11506613A (es)
AT (1) ATE211771T1 (es)
CA (1) CA2223823A1 (es)
DE (1) DE69618460T2 (es)
ES (1) ES2171679T3 (es)
WO (1) WO1996040996A1 (es)

Families Citing this family (27)

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US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification
SE504798C2 (sv) * 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
AR028149A1 (es) * 1999-07-08 2003-04-30 Innogenetics Nv Deteccion de la resistencia a los farmacos contra la hepatitis b
US6759193B2 (en) * 1999-07-28 2004-07-06 The Government Of The Republic Of Singapore Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip
EP1233976A4 (en) * 1999-11-17 2003-06-11 Jiuping Ji SIMULTANEOUS DETECTION OF HBV, HCV AND HIV IN PLASMA SAMPLES WITH THE AID OF A MULTIPLEX FIXING TEST
ES2170620B1 (es) * 1999-11-25 2003-12-16 Univ Alcala Henares Procedimiento de cuantificacion de acidos nucleicos mediante amplificacion por reaccion en cadena de polimerasa hemi-competitiva.
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
SG90149A1 (en) * 2000-07-18 2002-07-23 Government Of The Republic Of Diagnostic assay
ATE519861T1 (de) 2002-06-14 2011-08-15 Gen Probe Inc Zusammensetzungen zum nachweis von hepatitis-b- virus
KR100505419B1 (ko) * 2003-04-23 2005-08-04 주식회사 하이닉스반도체 반도체 소자의 소자분리막 제조방법
WO2005085475A1 (en) * 2004-03-01 2005-09-15 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
US20110117547A1 (en) * 2007-10-23 2011-05-19 Olympus Corporation Target dna detection method and target dna detection kit
EP2205617A4 (en) * 2007-11-06 2013-10-16 Siemens Healthcare Diagnostics HEPATITIS B-VIRUS-SPECIFIC OLIGONUCLEOTIDE SEQUENCES
CA2904181A1 (en) 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
EP3143401A4 (en) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Improved assay methods
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CN120098057A (zh) 2019-03-01 2025-06-06 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2049879A1 (en) * 1990-08-30 1992-03-01 Keith C. Backman Controlled initial target-dependent production of templates for ligase chain reaction
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
EP0633944B1 (en) * 1992-03-31 2000-11-08 Abbott Laboratories Method of multiplex ligase chain reaction
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
WO1994008047A1 (en) * 1992-09-25 1994-04-14 Abbott Laboratories Ligase chain reaction method for detecting small mutations
NZ256783A (en) * 1992-10-01 1996-08-27 Union Camp Patent Holding Bleaching high consistency lignocellulosic pulp from a first ge brightness to a second higher ge brightness by fluffing the pulp to a specific surface area of at least 90m2kg-1 followed by treatment with a gaseous bleaching agent
EP0785996A1 (en) * 1993-07-13 1997-07-30 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification

Also Published As

Publication number Publication date
WO1996040996A1 (en) 1996-12-19
CA2223823A1 (en) 1996-12-19
EP0832281B1 (en) 2002-01-09
JPH11506613A (ja) 1999-06-15
ATE211771T1 (de) 2002-01-15
DE69618460T2 (de) 2002-09-26
US5667974A (en) 1997-09-16
DE69618460D1 (de) 2002-02-14
EP0832281A1 (en) 1998-04-01
US5955598A (en) 1999-09-21

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