ATE211771T1 - Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation - Google Patents

Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation

Info

Publication number
ATE211771T1
ATE211771T1 AT96917000T AT96917000T ATE211771T1 AT E211771 T1 ATE211771 T1 AT E211771T1 AT 96917000 T AT96917000 T AT 96917000T AT 96917000 T AT96917000 T AT 96917000T AT E211771 T1 ATE211771 T1 AT E211771T1
Authority
AT
Austria
Prior art keywords
target
nucleic acid
acid sequence
test sample
acid sequences
Prior art date
Application number
AT96917000T
Other languages
English (en)
Inventor
Larry Birkenmeyer
Isa K Mushahwar
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE211771T1 publication Critical patent/ATE211771T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT96917000T 1995-06-07 1996-06-03 Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation ATE211771T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/480,220 US5667974A (en) 1995-06-07 1995-06-07 Method for detecting nucleic acid sequences using competitive amplification
PCT/US1996/008429 WO1996040996A1 (en) 1995-06-07 1996-06-03 Method for detecting nucleic acid sequences using competitive amplification

Publications (1)

Publication Number Publication Date
ATE211771T1 true ATE211771T1 (de) 2002-01-15

Family

ID=23907134

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96917000T ATE211771T1 (de) 1995-06-07 1996-06-03 Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation

Country Status (8)

Country Link
US (2) US5667974A (de)
EP (1) EP0832281B1 (de)
JP (1) JPH11506613A (de)
AT (1) ATE211771T1 (de)
CA (1) CA2223823A1 (de)
DE (1) DE69618460T2 (de)
ES (1) ES2171679T3 (de)
WO (1) WO1996040996A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification
SE504798C2 (sv) * 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) * 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
JP4468488B2 (ja) * 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AR028149A1 (es) * 1999-07-08 2003-04-30 Innogenetics Nv Deteccion de la resistencia a los farmacos contra la hepatitis b
US6759193B2 (en) 1999-07-28 2004-07-06 The Government Of The Republic Of Singapore Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip
CA2392218A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
ES2170620B1 (es) * 1999-11-25 2003-12-16 Univ Alcala Henares Procedimiento de cuantificacion de acidos nucleicos mediante amplificacion por reaccion en cadena de polimerasa hemi-competitiva.
CA2405412A1 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
SG90149A1 (en) * 2000-07-18 2002-07-23 Government Of The Republic Of Diagnostic assay
AU2003253651C1 (en) * 2002-06-14 2010-06-03 Gen-Probe Incorporated Compositions and methods for detecting hepatitis B virus
KR100505419B1 (ko) * 2003-04-23 2005-08-04 주식회사 하이닉스반도체 반도체 소자의 소자분리막 제조방법
EP1721011A1 (de) * 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
JPWO2009054367A1 (ja) * 2007-10-23 2011-03-03 オリンパス株式会社 標的ｄｎａの検出方法及び標的ｄｎａ検出キット
EP2205617A4 (de) * 2007-11-06 2013-10-16 Siemens Healthcare Diagnostics Hepatitis-b-virus-spezifische oligonukleotidsequenzen
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
EP2971183A4 (de) 2013-03-13 2016-10-26 Meso Scale Technologies Llc Verbesserte testverfahren
KR20250011724A (ko) 2014-05-15 2025-01-21 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CN114144422B (zh) 2019-03-01 2025-03-07 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2049879A1 (en) * 1990-08-30 1992-03-01 Keith C. Backman Controlled initial target-dependent production of templates for ligase chain reaction
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
EP1018649A3 (de) * 1992-03-31 2000-11-29 Abbott Laboratories Immunchromatographisches Gerät sowie Verfahren zur Mehrfachdetektion unterschiedlicher Analyten
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
AU4848393A (en) * 1992-09-25 1994-04-26 Abbott Laboratories Ligase chain reaction method for detecting small mutations
WO1994008087A1 (en) * 1992-10-01 1994-04-14 Union Camp Patent Holding, Inc. Improved bleaching of high consistency lignocellulosic pulp
EP0785996A1 (de) * 1993-07-13 1997-07-30 Abbott Laboratories Nukleotid sequenzen und verfahren zur vervielfältigung und bestimmung von hepatitus-b-virus
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification

Also Published As

Publication number Publication date
EP0832281B1 (de) 2002-01-09
ES2171679T3 (es) 2002-09-16
DE69618460T2 (de) 2002-09-26
US5955598A (en) 1999-09-21
JPH11506613A (ja) 1999-06-15
CA2223823A1 (en) 1996-12-19
WO1996040996A1 (en) 1996-12-19
EP0832281A1 (de) 1998-04-01
US5667974A (en) 1997-09-16
DE69618460D1 (de) 2002-02-14

Similar Documents

Publication Publication Date Title
ATE211771T1 (de) Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation
ATE215993T1 (de) Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat- primer-reihen
MY104957A (en) Method detecting nucleotide sequences.
ATE142272T1 (de) Nukleinsäure-amplifikation unter verwendung eines einzelprimers
CA2236067A1 (en) Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples
DE59606675D1 (de) Verfahren zum nachweis von telomerase-aktivität
DE69718268D1 (de) Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse
FI950836A0 (fi) Väärien negatiivien tulosten eliminointi nukleiinihapon toteamisessa
ATE264923T1 (de) Verfahren und system zur erkennung von oligonukleotiden in einer probe
ATE184055T1 (de) Verfahren zum sensitiven nachweis von reverser transcriptase
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
DK0795612T3 (da) Mangfoldiggørelse og påvisning af målnukleinsyrer under anvendelse af et postmangfoldiggørelses-inkubationstrin
EP0950718A4 (de) Verfahren zur detektion von dna mittels einer pna-sonde
ATE232981T1 (de) Differenziertes timingsverfahren zum nachweiss multiple analyte in eine testprobe
DK0677589T3 (da) Fremgangsmåde, testelement og testkit til semikvntitativ påvisning af mål-nucleinsyre
CA2211165A1 (en) Methods for sensitive detection of reverse transcriptase

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee