ES2173310T3 - Colorante con separadores universales/de transferencia de energia. - Google Patents

Colorante con separadores universales/de transferencia de energia.

Info

Publication number
ES2173310T3
ES2173310T3 ES96928154T ES96928154T ES2173310T3 ES 2173310 T3 ES2173310 T3 ES 2173310T3 ES 96928154 T ES96928154 T ES 96928154T ES 96928154 T ES96928154 T ES 96928154T ES 2173310 T3 ES2173310 T3 ES 2173310T3
Authority
ES
Spain
Prior art keywords
energy transfer
compositions
replaced
union
monomeric units
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96928154T
Other languages
English (en)
Inventor
Richard Mathies
Alexander N Glazer
Jingyue Ju
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Application granted granted Critical
Publication of ES2173310T3 publication Critical patent/ES2173310T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE PROPORCIONAN MARCADORES FLUORESCENTES QUE UTILIZAN COMPONENTES ABSORBENTES/DONANTES DE ENERGIA UNIDOS ENTRE SI MEDIANTE UN ESPACIADOR, EL CUAL CONTIENE UNIDADES MONOMERICAS DE AZUCAR FOSFATO, ESPECIALMENTE UNIDADES MONOMERICAS DE RIBOSIL FOSFATO, DONDE LOS GRUPOS AZUCAR ESTAN LIBRES DE GRUPOS HIDROXILO ACTIVOS. PARTICULARMENTE, UN COMPONENTE DE TRANSFERENCIA DE ENERGIA ESTA SUSTITUIDO EN LA POSICION 5 DE LA CADENA ESPACIADORA, MIENTRAS QUE EL OTRO COMPONENTE DE TRANSFERENCIA DE ENERGIA ESTA SUSTITUIDO EN LA POSICION 1 DEL GRUPO RIBOSILO 3 TERMINAL DEL MARCADOR FORMANDO UN CASETTE ET PARA LA UNION A UNA SECUENCIA DE ACIDO NUCLEICO CON CUALQUIERA DE LAS COMPOSICIONES. UTILIZANDO LAS COMBINACIONES DE LOS COMPONENTES ET, CON UN ABSORBENTE/DONANTE DE ENERGIA COMUN Y DIFERENTES FLUORESCENTES, SE PUEDEN PROPORCIONAR FAMILIAS DE MARCADORES QUE SE PUEDEN UNIR A CUALQUIER MOLECULA DIANA Y LAS CUALES PUEDEN SER EXCITADAS EN UNA LONGITUD DE ONDA INDIVIDUAL Y HACERSE FLUORESCENTES CON DIFERENTES LONGITUDES DE ONDA CON DESPLAZAMIENTOS STOKES GRANDES. LAS COMPOSICIONES TIENEN UNA APLICACION PARTICULAR PARA LA SECUENCIACION.
ES96928154T 1995-09-20 1996-08-12 Colorante con separadores universales/de transferencia de energia. Expired - Lifetime ES2173310T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/531,132 US5728528A (en) 1995-09-20 1995-09-20 Universal spacer/energy transfer dyes

Publications (1)

Publication Number Publication Date
ES2173310T3 true ES2173310T3 (es) 2002-10-16

Family

ID=24116371

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96928154T Expired - Lifetime ES2173310T3 (es) 1995-09-20 1996-08-12 Colorante con separadores universales/de transferencia de energia.

Country Status (7)

Country Link
US (1) US5728528A (es)
EP (1) EP0851867B1 (es)
JP (1) JP3066984B2 (es)
CA (1) CA2231475C (es)
DE (1) DE69618649T2 (es)
ES (1) ES2173310T3 (es)
WO (1) WO1997011084A1 (es)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US7388092B2 (en) * 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7745142B2 (en) * 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) * 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US20050227294A1 (en) * 1997-09-15 2005-10-13 Molecular Devices Corporation Molecular modification assays involving lipids
US6007992A (en) * 1997-11-10 1999-12-28 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US6028183A (en) * 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
AU2571899A (en) * 1998-02-03 1999-08-16 Amersham Pharmacia Biotech Inc. Energy transfer dyes
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
GB2341189B (en) * 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US7209836B1 (en) 1999-07-16 2007-04-24 Perkinelmer Las, Inc. Method and system for automatically creating crosstalk-corrected data of a microarray
JP2001033427A (ja) * 1999-07-16 2001-02-09 Hitachi Software Eng Co Ltd 電気泳動方法及び電気泳動装置
US7138254B2 (en) 1999-08-02 2006-11-21 Ge Healthcare (Sv) Corp. Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins
US6218124B1 (en) 1999-08-27 2001-04-17 Pe Corporation Method for detecting oligonucleotides using UV light source
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
JP2003527833A (ja) 1999-10-14 2003-09-24 クロンテック・ラボラトリーズ・インコーポレーテッド 花虫類に由来する発色団／蛍光体、およびそれらの使用法
IL149676A0 (en) 1999-12-02 2002-11-10 Dna Sciences Inc Methods and kits for determining nucleotide variations
US6528318B1 (en) * 2000-03-06 2003-03-04 The Johns Hopkins University Scatter controlled emission for optical taggants and chemical sensors
US6436641B1 (en) * 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
US6355433B1 (en) 2000-06-02 2002-03-12 Dna Sciences, Inc. Determination of nucleotide sequence variations through limited primer extension
US6627748B1 (en) * 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
WO2003054158A2 (en) * 2001-12-19 2003-07-03 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
EP1405920A3 (en) * 2002-10-02 2004-04-14 Roche Diagnostics GmbH Improved FRET process
US20070184453A1 (en) * 2002-10-02 2007-08-09 Roche Molecular Systems, Inc Fret process
US7951923B2 (en) 2002-11-12 2011-05-31 Zakrytoe Aktsionernoe Obschestvo ‘Evrogen’ Fluorescent proteins and chromoproteins from non-Aequorea hydrozoa species and methods for using same
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
ATE474922T1 (de) 2002-12-26 2010-08-15 Zakrytoe Aktsionernoe Obschest Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
WO2005112544A2 (en) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
EP2436778A3 (en) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
EP1732944B1 (en) * 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
SG155923A1 (en) 2004-09-16 2009-10-29 Applied Biosystems Llc Fluorescent dye compounds, conjugates and uses thereof
JP2008541055A (ja) * 2005-05-03 2008-11-20 アプレラ コーポレイション 蛍光検出システムおよびそれとともに使用するための色素セット
US9169510B2 (en) * 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
ES2399563T3 (es) * 2005-11-04 2013-04-02 Evrogen, Jsc Proteínas verdes fluorescentes modificadas y método para la utilización de las mismas
WO2007062105A2 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
WO2007085923A2 (en) 2006-01-25 2007-08-02 Evrogen, Ip Novel fluorescent proteins and methods for using same
EP2020988B1 (en) 2006-04-28 2017-08-16 Children's Hospital Medical Center Compositions comprising fusogenic proteins or polypeptides derived from prosaposin for application in transmembrane drug delivery systems
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
WO2009051807A1 (en) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
WO2009059305A2 (en) * 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2012090073A2 (en) 2010-12-30 2012-07-05 The Netherlands Cancer Institute Methods and compositions for predicting chemotherapy sensitivity
US20140296248A1 (en) 2011-04-04 2014-10-02 Stichting het Nederlands Kanker Instiuut-Antoni van Leeuwenhoek ziekenhuis Methods and compositions for predicting resistance to anticancer treatment
EP2694678A2 (en) 2011-04-04 2014-02-12 Netherland Cancer Institute Methods and compositions for predicting resistance to anticancer treatment
WO2012149038A1 (en) 2011-04-25 2012-11-01 Advanced Bioscience Laboratories, Inc. Truncated hiv envelope proteins (env), methods and compositions related thereto
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
WO2014139596A1 (en) 2013-03-15 2014-09-18 Illumina Cambridge Limited Modified nucleosides or nucleotides
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9849198B2 (en) 2013-07-02 2017-12-26 The Board Of Trustees Of The Leland Stanford Junior University Discrete imaging of hepatic oxidative and nitrosative stress with two-channel nanoparticles for in vivo drug safety screening
WO2018057648A1 (en) 2016-09-20 2018-03-29 The Board Of Trustees Of The Leland Stanford Junior University Peptide regulators of mitochondrial fusion and methods of use
WO2018200323A1 (en) 2017-04-23 2018-11-01 Washington University Small molecule regulators of mitochondrial fusion and methods of use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
DE4119075A1 (de) * 1991-06-10 1992-12-17 Max Planck Gesellschaft Nucleosidtriphosphorsaeureester und deren verwendung
EP1067134B1 (en) * 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5843658A (en) * 1995-03-10 1998-12-01 Hamamatsu Photonics K.K. Method of measuring oligonucleotide decomposing activity

Also Published As

Publication number Publication date
WO1997011084A1 (en) 1997-03-27
DE69618649D1 (de) 2002-02-28
US5728528A (en) 1998-03-17
CA2231475A1 (en) 1997-03-27
EP0851867A1 (en) 1998-07-08
JPH10511987A (ja) 1998-11-17
DE69618649T2 (de) 2002-08-29
CA2231475C (en) 2002-02-05
EP0851867B1 (en) 2002-01-02
JP3066984B2 (ja) 2000-07-17

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