ES2175343T3 - Aparato de electroporacion que emplea pulsos configurados por el usuario. - Google Patents

Aparato de electroporacion que emplea pulsos configurados por el usuario.

Info

Publication number
ES2175343T3
ES2175343T3 ES97903934T ES97903934T ES2175343T3 ES 2175343 T3 ES2175343 T3 ES 2175343T3 ES 97903934 T ES97903934 T ES 97903934T ES 97903934 T ES97903934 T ES 97903934T ES 2175343 T3 ES2175343 T3 ES 2175343T3
Authority
ES
Spain
Prior art keywords
cells
duration
cell
molecules
low voltage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97903934T
Other languages
English (en)
Inventor
Gunter A Hofmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetronics Inc
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Application granted granted Critical
Publication of ES2175343T3 publication Critical patent/ES2175343T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H03K3/02—Generators characterised by the type of circuit or by the means used for producing pulses
    • H03K3/53—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback
    • H03K3/57—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback the switching device being a semiconductor device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrotherapy Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Percussion Or Vibration Massage (AREA)

Abstract

UN PROCEDIMIENTO Y APARATO DE ELECTROPORACION PARA LA GENERACION Y APLICACION DE UN CAMPO MAGNETICO SEGUN UN SISTEMA DE IMPULSOS CONFIGURADO POR EL USUARIO. CONVENIENTEMENTE, UNO DE TALES IMPULSOS COMPRENDE UN IMPULSO DE BAJA TENSION DE UNA PRIMERA DURACION, SEGUIDO INMEDIATAMENTE POR UNO DE ALTA TENSION DE UNA SEGUNDA DURACION, SEGUIDO INMEDIATAMENTE POR UNO DE BAJA TENSION DE UNA TERCERA DURACION. EL CAMPO DE ELECTROPORACION DE BAJA TENSION ACUMULA MOLECULAS EN LA SUPERFICIE DE UNA CELULA, CREANDO CONVENIENTEMENTE EL CAMPO DE ALTA TENSION UNA ABERTURA EN LA CELULA Y MOVIENDO EL CAMPO DE BAJA TENSION FINAL LA MOLECULA AL INTERIOR DE LA CELULA. LAS MOLECULAS PUEDEN SER ADN, PORCIONES DE ADN, AGENTES QUIMICOS, LAS CELULAS RECEPTORAS PUEDEN SER HUEVOS, PLAQUETAS, CELULAS HUMANAS, GLOBULOS ROJOS, CELULAS DE MAMIFEROS, PROTOPLASTOS DE PLANAS, POLEN DE PLANTAS, LIPOSOMAS, BACTERIAS, HONGOS, LEVADURA, ESPERMA U OTRAS CELULAS ADECUADAS. LAS MOLECULAS SE COLOCAN CERCA DE LAS CELULAS, BIEN EN EL ESPACIO INTERSTICIAL DE LOS TEJIDOS QUE RODEAN LAS CELULAS O EN UN MEDIO LIQUIDO QUE CONTENGA LAS CELULAS.
ES97903934T 1996-09-09 1997-01-27 Aparato de electroporacion que emplea pulsos configurados por el usuario. Expired - Lifetime ES2175343T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/709,615 US5869326A (en) 1996-09-09 1996-09-09 Electroporation employing user-configured pulsing scheme

Publications (1)

Publication Number Publication Date
ES2175343T3 true ES2175343T3 (es) 2002-11-16

Family

ID=24850605

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97903934T Expired - Lifetime ES2175343T3 (es) 1996-09-09 1997-01-27 Aparato de electroporacion que emplea pulsos configurados por el usuario.

Country Status (9)

Country Link
US (2) US5869326A (es)
EP (1) EP0925647B1 (es)
JP (1) JP3963955B2 (es)
KR (1) KR100420731B1 (es)
AT (1) ATE216155T1 (es)
CA (1) CA2261703A1 (es)
DE (1) DE69711893T2 (es)
ES (1) ES2175343T3 (es)
WO (1) WO1998010515A1 (es)

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US5869326A (en) 1999-02-09
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ATE216155T1 (de) 2002-04-15
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