ES2186768T3 - Procedimiento para la purificacion de acidos nucleicos cortos. - Google Patents

Procedimiento para la purificacion de acidos nucleicos cortos.

Info

Publication number
ES2186768T3
ES2186768T3 ES96901592T ES96901592T ES2186768T3 ES 2186768 T3 ES2186768 T3 ES 2186768T3 ES 96901592 T ES96901592 T ES 96901592T ES 96901592 T ES96901592 T ES 96901592T ES 2186768 T3 ES2186768 T3 ES 2186768T3
Authority
ES
Spain
Prior art keywords
oligonucleotide
pct
purification
adsorbent
sec
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96901592T
Other languages
English (en)
Inventor
Hans Johansson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB filed Critical Amersham Bioscience AB
Application granted granted Critical
Publication of ES2186768T3 publication Critical patent/ES2186768T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Sludge (AREA)

Abstract

METODO PARA LA PURIFICACION DE UN OLIGONUCLEOTIDO SINTETICO A PARTIR DE SECUENCIAS ERRONEAS QUE CONSISTE EN PONER EN CONTACTO UNA MUESTRA QUE CONTENGA EL OLIGONUCLEOTIDO DESEADO EN FORMA SOLUBLE AL AGUA PROTEGIDA CON UN ADSORBENTE HIDROFILO QUE CONTENGA GRUPOS DE INTERCAMBIO DE ANIONES BAJO CONDICIONES QUE PERMITAN EL ENLACE DE DICHO OLIGONUCLEOTIDO A DICHO ADSORBENTE, TRAS LO CUAL SE DESPROTEGE EL OLIGONUCLEOTIDO ADSORBIDO Y SE SEPARA DE LAS SECUENCIAS ERRONEAS. LA CARACTERISTICA PARTICULAR DEL METODO ES LA UTILIZACION DE UN ADSORBENTE DE INTERCAMBIO DE IONES QUE SE UNA AL OLIGONUCLEOTIDO PROTEGIDO BAJO CONDICIONES TANTO DE ALTA COMO DE BAJA RESISTENCIA IONICA.
ES96901592T 1995-01-20 1996-01-18 Procedimiento para la purificacion de acidos nucleicos cortos. Expired - Lifetime ES2186768T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9500183A SE9500183D0 (sv) 1995-01-20 1995-01-20 Method for the purifivation of short nucleic acids

Publications (1)

Publication Number Publication Date
ES2186768T3 true ES2186768T3 (es) 2003-05-16

Family

ID=20396889

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96901592T Expired - Lifetime ES2186768T3 (es) 1995-01-20 1996-01-18 Procedimiento para la purificacion de acidos nucleicos cortos.

Country Status (10)

Country Link
US (1) US5801237A (es)
EP (1) EP0804448B1 (es)
JP (1) JP4148991B2 (es)
AT (1) ATE225362T1 (es)
AU (1) AU686045B2 (es)
CA (1) CA2210855A1 (es)
DE (1) DE69624096T2 (es)
ES (1) ES2186768T3 (es)
SE (1) SE9500183D0 (es)
WO (1) WO1996022299A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990064332A (ko) 1995-10-19 1999-07-26 래리 더블유. 스미스, 코크란 아담 올리고뉴클레오티드의 액상 합성법
AU744950B2 (en) * 1998-02-03 2002-03-07 Arqule, Inc. Rapid method for separation of small molecules using reverse phase high performance liquid chromatography
US6969587B2 (en) * 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US7347976B2 (en) * 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US20030153741A1 (en) * 2002-02-08 2003-08-14 Marek Kwiatkowski Methods for separating oligonucleotides
US7981600B2 (en) * 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US20050142570A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7274852B1 (en) * 2005-12-02 2007-09-25 Adc Telecommunications, Inc. Splice tray arrangement
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
EP4070102A1 (en) 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
EP4182460A1 (en) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US5183885A (en) * 1991-10-18 1993-02-02 Applied Biosystems, Inc. Method for chromatographic separation of synthetic phosphorothioate oligonucleotides
WO1995027718A2 (en) * 1994-04-08 1995-10-19 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
CA2194350A1 (en) * 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography

Also Published As

Publication number Publication date
DE69624096D1 (de) 2002-11-07
DE69624096T2 (de) 2003-05-28
ATE225362T1 (de) 2002-10-15
AU4592996A (en) 1996-08-07
EP0804448B1 (en) 2002-10-02
AU686045B2 (en) 1998-01-29
EP0804448A1 (en) 1997-11-05
CA2210855A1 (en) 1996-07-25
JP4148991B2 (ja) 2008-09-10
WO1996022299A1 (en) 1996-07-25
JPH10512566A (ja) 1998-12-02
US5801237A (en) 1998-09-01
SE9500183D0 (sv) 1995-01-20

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